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Invitrogen
Osteocalcin Polyclonal Antibody
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Osteocalcin Antibody (PA5-78870) in IHC (P)
Immunohistochemistry analysis of Osteocalcin on paraffin-embedded mouse tibia tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with Osteocalcin polyclonal antibody (Product# PA5-78870) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°... View More
Product Details
PA5-78870
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived mouse Osteocalcin recombinant protein (Position: Y50-I95).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745986
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - IHC: mouse tibia tissue.
Target Information
Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BGP; bone gamma carboxyglutamate protein 1; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein; OG1; Osteocalcin; PMF1; RP11-54H19.5
Gene Aliases: Bglap; Bglap1; mOC-A; OC; Ocn; OG1
UniProt ID: (Mouse) P86546
Entrez Gene ID: (Mouse) 12096
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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