Invitrogen

Osteocalcin Polyclonal Antibody

1 Published Figure

3 References

View all (31) Osteocalcin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (1)

Osteocalcin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

Osteocalcin Antibody (PA5-96529) in ICC/IF

Immunofluorescence analysis of Osteocalcin in NIH/3T3 cells. Samples were incubated with Osteocalcin Polyclonal antibody (Product # PA5-96529) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Osteocalcin Antibody in Western Blot (WB)

Product Image Gallery

Product Details

PA5-96529

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (IHC)

- -

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:200

View 2 publications 2 publications

ELISA (ELISA)

1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Osteocalcin (BGLAP) (NP_9546421)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2808331

Product Specific Information

Immunogen sequence: MRALTLLALL ALAALCIAGQ AGAKPSGAES SKGAAFVSKQ EGSEVVKRPR RYLYQWLGAP VPYPDPLEPR REVCELNPDC DELADHIGFQ EAYRRFYGPV

Target Information

Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Magnetic Field Boosts the Transmembrane Transport Efficiency of Magnesium Ions from PLLA Bone Scaffold.

Small (Weinheim an der Bergstrasse, Germany)

Yan Z,Sun T,Tan W,Wang Z,Yan J,Miao J,Wu X,Feng P,Deng Y

PA5-96529 was used in Immunocytochemistry to show that the magnetic magnesium-loaded scaffold, under the action of SMF, can accelerate the inflow of Mg2+ from surrounding microenvironment, which improved cellular activities, osteogenesis-related gene expression (ALP, Runx2, OCN, and OPN), and mineralization.

Sun Oct 01 00:00:00 UTC 2023

3D-printed NIR-responsive shape memory polyurethane/magnesium scaffolds with tight-contact for robust bone regeneration.

Bioactive materials

Zhang Y,Li C,Zhang W,Deng J,Nie Y,Du X,Qin L,Lai Y

PA5-96529 was used in Immunohistochemistry (Paraffin) to report a near-infrared-responsive and tight-contacting scaffold that comprises of shape memory polyurethane (SMPU) as the thermal-responsive matrix and magnesium (Mg) as the photothermal and bioactive component, which fabricated by the low temperature rapid prototyping (LT-RP) 3D printing technology.

Sat Oct 01 00:00:00 UTC 2022

Mechanics-driven nuclear localization of YAP can be reversed by N-cadherin ligation in mesenchymal stem cells.

Nature communications

Zhang C,Zhu H,Ren X,Gao B,Cheng B,Liu S,Sha B,Li Z,Zhang Z,Lv Y,Wang H,Guo H,Lu TJ,Xu F,Genin GM,Lin M

PA5-96529 was used in Immunocytochemistry to demonstrate that controlled storage and removal of proteins associated with mechanical memory in mesenchymal stem cells is possible through defined and programmable material systems.

Thu Oct 28 00:00:00 UTC 2021

Mechanics-driven nuclear localization of YAP can be reversed by N-cadherin ligation in mesenchymal stem cells.

Nature communications

Zhang C,Zhu H,Ren X,Gao B,Cheng B,Liu S,Sha B,Li Z,Zhang Z,Lv Y,Wang H,Guo H,Lu TJ,Xu F,Genin GM,Lin M

Thu Oct 28 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: BGP; bone gamma carboxyglutamate protein 1; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate protein 2; bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin); Bone Gla protein; bone Gla-protein; Gamma-carboxyglutamic acid-containing protein; OG1; Osteocalcin; PMF1; RP11-54H19.5

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Gene Aliases: BGLAP; Bglap1; Bglap2; BGP; Bgpr; Bgpra; mOC-A; OC; OCN; OG1

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UniProt ID: (Human) P02818, (Mouse) P86546, (Rat) P04640

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Entrez Gene ID: (Human) 632, (Mouse) 12096, (Rat) 25295

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Function(s)

structural molecule activity calcium ion binding structural constituent of bone hydroxyapatite binding metal ion binding

Process(es)

regulation of bone mineralization biomineral tissue development cellular response to BMP stimulus skeletal system development osteoblast differentiation osteoblast development signal peptide processing ER to Golgi vesicle-mediated transport cell adhesion cell aging response to mechanical stimulus response to gravity response to zinc ion response to activity peptidyl-glutamic acid carboxylation bone mineralization response to vitamin K response to vitamin D response to testosterone response to hydroxyisoflavone odontogenesis response to drug response to estrogen regulation of bone resorption response to ethanol regulation of osteoclast differentiation response to glucocorticoid bone development cellular response to vitamin D cellular response to growth factor stimulus ossification aging response to inorganic substance response to organic cyclic compound response to nutrient levels

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Osteocalcin Polyclonal Antibody

Osteocalcin Antibody (PA5-96529) in ICC/IF

Product Details

PA5-96529

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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