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Invitrogen
Osteocalcin Monoclonal Antibody (OC4-30)
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Osteocalcin Antibody (33-5400) in WB
International journal of molecular medicine 2020 -
Figure 8 Endogenous protein expression of Runx2, COL1 and OCN during osteogenic differentiation of cPDLSCs. (A) Western blot analysis of protein expression levels of Runx2, COL1 and OCN in Vector-cPDLSCs and EfnB2-cPDLSCs after 7, 14 and 21 days of osteogenic/odontogenic induction. Quantification bands of (B) Runx2, (C) COL1 and (D) OCN in Vector-cPDLSCs and EfnB2-cPDLSCs. * P<0.05 vs. Vector-cPDLSCs. C... View More
Product Details
33-5400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Goat,
Human,
Sheep,
Pig,
Rabbit,
Rat
Published species
Dog,
Human,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OC4-30
Immunogen
Full-length Bovine osteocalcin
Conjugate
Form
Lyophilized
Concentration
2.0 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533125
Product Specific Information
Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3U1 with spleen cells of BALB/c mouse after immunization with bovine osteocalcin.
Specificity and Subclass: Epitope gamma-carboxylated residue at position 17 (does not react with decarboxylated osteocalcin.)
Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.
Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.
Target Information
Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BGP; bone gamma-carboxyglutamate (gla) protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate (gla) protein-like; bone gamma-carboxyglutamate protein 2; bone gamma-carboxyglutamate protein osteocalcin; bone gamma-carboxyglutamate; BGLAP; bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin); Bone Gla protein; bone Gla-protein; gamma-carbooxyglutamate (gla) protein; Gamma-carboxyglutamic acid-containing protein; Osteocalcin; PMF1; RP11-54H19.5
Gene Aliases: BGLAP; Bglap2; BGP; Bgpr; Bgpra; BOS_2768; GLA; OC; OCN
UniProt ID: (Human) P02818, (Bovine) P02820, (Rat) P04640
Entrez Gene ID: (Dog) 403762, (Chicken) 396348, (Sheep) 677670, (Human) 632, (Bovine) 281646, (Pig) 397530, (Goat) 100861188, (Rat) 25295, (Rabbit) 100340503
regulation of bone mineralization
biomineral tissue development
skeletal system development
osteoblast differentiation
osteoblast development
signal peptide processing
ER to Golgi vesicle-mediated transport
cell adhesion
cell aging
response to mechanical stimulus
response to gravity
response to zinc ion
response to activity
peptidyl-glutamic acid carboxylation
bone mineralization
response to vitamin K
response to vitamin D
response to testosterone
response to hydroxyisoflavone
odontogenesis
response to drug
response to estrogen
regulation of bone resorption
response to ethanol
regulation of osteoclast differentiation
response to glucocorticoid
bone development
cellular response to vitamin D
cellular response to growth factor stimulus
ossification
aging
response to inorganic substance
response to organic cyclic compound
response to nutrient levels
cellular response to BMP stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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