Invitrogen

Osteopontin Polyclonal Antibody

Osteopontin Antibody in Immunocytochemistry (ICC/IF)

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Osteopontin Antibody (PA1-25152) in ICC/IF

Oncotarget 2016 - Figure 1 CTD-deficient cells display altered properties in their cell behaviour and phenotype ( A ) CTD-deficient mice (DeltaT) express a carboxyl-terminal truncated Cx43 (K258Stop) instead of the WT Cx43 isoform [ 32 ]. ( B and C ) Mouse genotyping was performed by PCR and confirmed by western blot using an antibody raised against the N-terminal region of Cx43, in order to detect the full length (+/+ and +... View More

Osteopontin Antibody in Neutralization (Neu)

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Product Details

PA1-25152

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

Immunohistochemistry (IHC)

5-15 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

0.2-0.8 µg/mL

Neutralization (Neu)

1-3 µg/mL

View 1 publication 1 publication

Inhibition Assays (IA)

1-3 µg/mL

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified recombinant mouse osteopontin expressed in NSO cells.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_795087

Product Specific Information

By Western Blot, a doublet band of approximately 65 kDa and a band of 30 kDa is observed due to glycosylation. A suggested positive control is 293 cells. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/mL in the presence of 2 µg/mL of recombinant mouse osteopontin using 293 cells.

Target Information

Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Blockage of Osteopontin-Integrin β3 Signaling in Infrapatellar Fat Pad Attenuates Osteoarthritis in Mice.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Dai B,Zhu Y,Li X,Liang Z,Xu S,Zhang S,Zhang Z,Bai S,Tong W,Cao M,Li Y,Zhu X,Liu W,Zhang Y,Chang L,Yung PS,Ki-Wai Ho K,Xu J,Ngai T,Qin L

Published figure using Osteopontin polyclonal antibody (Product # PA1-25152) in Neutralization

Tue Aug 01 00:00:00 UTC 2023

Macrophages in epididymal adipose tissue secrete osteopontin to regulate bone homeostasis.

Nature communications

Dai B,Xu J,Li X,Huang L,Hopkins C,Wang H,Yao H,Mi J,Zheng L,Wang J,Tong W,Chow DH,Li Y,He X,Hu P,Chen Z,Zu H,Li Y,Yao Y,Jiang Q,Qin L

PA1-25152 was used in Immunohistochemistry to show that epididymal white adipose tissue-secreted osteopontin induces lipophagocytic mobilization of bone marrow-derived macrophages (BMDMs) to a lipid-rich pool in the bone marrow, promotes bone matrix degradation by activating osteoclasts in high-fat diet-fed mice, and blocks both lipolysis and osteoclastogenesis in BMDMs by limiting the energy regeneration by ATP6V0d2 in lysosomes.

Thu Jan 20 00:00:00 UTC 2022

Transcriptome profiling of brain myeloid cells revealed activation of Itgal, Trem1, and Spp1 in western diet-induced obesity.

Journal of neuroinflammation

Yang H,Graham LC,Reagan AM,Grabowska WA,Schott WH,Howell GR

PA1-25152 was used in Immunohistochemistry-immunofluorescence to support the model that peripheral myeloid cells enter the brain in response to diet-induced obesity.

Mon Aug 19 00:00:00 UTC 2019

The C-terminal domain of connexin43 modulates cartilage structure via chondrocyte phenotypic changes.

Oncotarget

Gago-Fuentes R,Bechberger JF,Varela-Eirin M,Varela-Vazquez A,Acea B,Fonseca E,Naus CC,Mayan MD

PA125152 was used in immunocytochemistry to examine chondrocyte from mice deficient in the carboxy terminal domain of connexin 43

Tue Nov 08 00:00:00 UTC 2016

Pannexin 3 is a novel target for Runx2, expressed by osteoblasts and mature growth plate chondrocytes.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Bond SR,Lau A,Penuela S,Sampaio AV,Underhill TM,Laird DW,Naus CC

PA1-25152 was used in immunohistochemistry to study the expression of pannexin-3 in osteoblasts and mature growth plate chondrocytes and its role as a target of Runx2 signaling

Thu Dec 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: 2AR; 44 kDa bone phosphoprotein; Bone sialoprotein 1; Bone sialoprotein 1 (BSPI/BSP1); Calcium oxalate crystal growth inhibitor protein; CALPHA1 fusion; Early T lymphocyte activation 1 (ETA1); Early T-lymphocyte activation 1 protein; HGNC:11255; immunoglobulin alpha 1 heavy chain constant region fusion protein; MGC110940; Minopontin; Osteopontin; osteopontin-like protein; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); Secreted phosphoprotein 1 (SPP1); SPP-1; urinar

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Gene Aliases: 2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1; OP; Opn; Opnl; Ric; Spp-1; Spp1

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UniProt ID: (Mouse) P10923

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Entrez Gene ID: (Mouse) 20750

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Function(s)

cytokine activity extracellular matrix binding

Process(es)

ossification osteoblast differentiation cell adhesion response to organic substance positive regulation of cell-substrate adhesion neutrophil chemotaxis biomineral tissue development response to vitamin D negative regulation of apoptotic process positive regulation of bone resorption response to steroid hormone cellular response to fluid shear stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Osteopontin Polyclonal Antibody

Osteopontin Antibody (PA1-25152) in ICC/IF

Product Details

PA1-25152

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Neutralization (Neu)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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