Invitrogen

P-Glycoprotein Monoclonal Antibody (F4)

P-Glycoprotein Antibody in Immunocytochemistry (ICC/IF)

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P-Glycoprotein Antibody (MA5-13854) in ICC/IF

Immunofluorescent analysis of p170 (green) showing staining in the membrane of A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a p170 monoclonal antibody (Product # MA5-13854) in 3% BSA-PBS at a diluti... View More

P-Glycoprotein Antibody in Western Blot (WB)

P-Glycoprotein Antibody in Flow Cytometry (Flow)

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Product Details

MA5-13854

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10-1:200

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 13 publications 13 publications

Flow Cytometry (Flow)

2 µg/test

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Immunogen

Human and hamster drug-resistant viable cells followed by crude plasma membranes

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10979045

Product Specific Information

MA5-13854 targets p170 in FACS, WB and ICC/IF applications and shows reactivity with Hamster and Human samples. This antibody does not react with mouse tissue in Western blot applications.

The MA5-13854 immunogen is human and hamster drug-resistant viable cells followed by crude plasma membranes.

Target Information

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chemical biology fluorescent tools for in vitro investigation of the multidrug resistant P-glycoprotein (P-gp) expression in tumor cells.

RSC advances

Daumar P,Goisnard A,Dubois C,Roux M,Depresle M,Penault-Llorca F,Bamdad M,Mounetou E

MA5-13854 was used in Immunocytochemistry-immunofluorescence to report the development of 25 novel fluorescent small-molecule conjugates with varying physicochemical and optical properties, and their biological evaluation in a cell model as P-gp targeted constructs.

Fri Sep 08 00:00:00 UTC 2023

Coxsackievirus B3 infects and disrupts human induced-pluripotent stem cell derived brain-like endothelial cells.

Frontiers in cellular and infection microbiology

Mamana J,Humber GM,Espinal ER,Seo S,Vollmuth N,Sin J,Kim BJ

MA5-13854 was used in Immunocytochemistry-immunofluorescence to elucidate the unique effects of CVB3 infection on the BBB and shed light on potential mechanisms by which the virus can initiate infections in the brain.

Fri May 05 00:00:00 UTC 2023

Characterization of resistant MCF-7 breast cancer cells developed by repeated cycles of photodynamic therapy.

Frontiers in pharmacology

Aniogo EC,George BP,Abrahamse H

Published figure using P-Glycoprotein monoclonal antibody (Product # MA5-13854) in Flow Cytometry

Tue Oct 04 00:00:00 UTC 2022

Characterization of resistant MCF-7 breast cancer cells developed by repeated cycles of photodynamic therapy.

Frontiers in pharmacology

Aniogo EC,George BP,Abrahamse H

Published figure using P-Glycoprotein monoclonal antibody (Product # MA5-13854) in Flow Cytometry

Tue Oct 04 00:00:00 UTC 2022

The Ubiquitin E3 Ligase Nedd4 Regulates the Expression and Amyloid-β Peptide Export Activity of P-Glycoprotein.

International journal of molecular sciences

Chai AB,Callaghan R,Gelissen IC

Published figure using P-Glycoprotein monoclonal antibody (Product # MA5-13854) in Western Blot

Tue Jan 18 00:00:00 UTC 2022

A microengineered Brain-Chip to model neuroinflammation in humans.

iScience

Pediaditakis I,Kodella KR,Manatakis DV,Le CY,Barthakur S,Sorets A,Gravanis A,Ewart L,Rubin LL,Manolakos ES,Hinojosa CD,Karalis K

MA5-13854 was used in Immunocytochemistry to present a human organotypic microphysiological system (MPS) that includes endothelial-like cells, pericytes, glia, and cortical neurons and maintains brain and blood-brain barrier permeability at in vivo relevant levels.

Fri Aug 19 00:00:00 UTC 2022

Mechanisms of P-Glycoprotein Regulation Under Exogenous and Endogenous Oxidative Stress In Vitro.

Acta naturae

Abalenikhina YV,Shchulkin AV,Mylnikov PY,Rokunov ED,Yakusheva EN

MA5-13854 was used in Western Blotting to investigate the mechanisms of P-glycoprotein (P-gp) transporter regulation in Caco-2 cells under exogenous and endogenous oxidative stress (OS).

Thu Nov 10 00:00:00 UTC 2022

Subcellular distribution of ezrin/radixin/moesin and their roles in the cell surface localization and transport function of P-glycoprotein in human colon adenocarcinoma LS180 cells.

PloS one

Kobori T,Tameishi M,Tanaka C,Urashima Y,Obata T

Published figure using P-Glycoprotein monoclonal antibody (Product # MA5-13854) in Immunocytochemistry

Tue Oct 26 00:00:00 UTC 2021

Subcellular distribution of ezrin/radixin/moesin and their roles in the cell surface localization and transport function of P-glycoprotein in human colon adenocarcinoma LS180 cells.

PloS one

Kobori T,Tameishi M,Tanaka C,Urashima Y,Obata T

Published figure using P-Glycoprotein monoclonal antibody (Product # MA5-13854) in Immunocytochemistry

Tue Oct 26 00:00:00 UTC 2021

Subcellular distribution of ezrin/radixin/moesin and their roles in the cell surface localization and transport function of P-glycoprotein in human colon adenocarcinoma LS180 cells.

PloS one

Kobori T,Tameishi M,Tanaka C,Urashima Y,Obata T

Published figure using P-Glycoprotein monoclonal antibody (Product # MA5-13854) in Immunocytochemistry

Tue Oct 26 00:00:00 UTC 2021

LightSpot^®-FL-1 Fluorescent Probe: An Innovative Tool for Cancer Drug Resistance Analysis by Direct Detection and Quantification of the P-glycoprotein (P-gp) on Monolayer Culture and Spheroid Triple Negative Breast Cancer Models.

Cancers

Goisnard A,Daumar P,Dubois C,Aubel C,Roux M,Depresle M,Gauthier J,Vidalinc B,Penault-Llorca F,Mounetou E,Bamdad M

MA5-13854 was used in Immunocytochemistry to establish a cancer detecting probe (detecting P-glyocoprotein) with Flow Cytometry and fluorescence microscopy.

Wed Aug 11 00:00:00 UTC 2021

Cancers

Goisnard A,Daumar P,Dubois C,Aubel C,Roux M,Depresle M,Gauthier J,Vidalinc B,Penault-Llorca F,Mounetou E,Bamdad M

MA5-13854 was used in Immunocytochemistry to establish a cancer detecting probe (detecting P-glyocoprotein) with Flow Cytometry and fluorescence microscopy.

Wed Aug 11 00:00:00 UTC 2021

Detection and analysis of multiple biomarkers in ovarian cancer: clinical significance in diagnosis, treatment, and prognosis evaluation.

Gland surgery

Ji R,Li Y,He C,Zhu X,He A,Lu Y

MA5-13854 was used in Immunohistochemistry (Paraffin) to explore the clinical significance of CA125, CK7, CK20, ER, PR, C-erbb2, and P-gp in ovarian cancer.

Tue Dec 01 00:00:00 UTC 2020

Duodenum Intestine-Chip for preclinical drug assessment in a human relevant model.

eLife

Kasendra M,Luc R,Yin J,Manatakis DV,Kulkarni G,Lucchesi C,Sliz J,Apostolou A,Sunuwar L,Obrigewitch J,Jang KJ,Hamilton GA,Donowitz M,Karalis K

Published figure using P-Glycoprotein monoclonal antibody (Product # MA5-13854) in Immunofluorescence

Tue Jan 14 00:00:00 UTC 2020

Long noncoding RNA lncARSR confers resistance to Adriamycin and promotes osteosarcoma progression.

Cell death & disease

Shen P,Cheng Y

MA5-13854 was used in Western Blotting to illustrate the role of long noncoding RNA ARSR (lncARSR) in the development of adapted ADM resistance.

Wed May 13 00:00:00 UTC 2020

In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves.

Journal of the peripheral nervous system : JPNS

Ouyang X,Dong C,Ubogu EE

MA5-13854 was used in Immunocytochemistry-immunoflourescence to investigate proteins specifically expressed by restrictive endoneurial microvascular endothelium in four adult sural nerves.

Sat Jun 01 00:00:00 UTC 2019

Combined effects of octreotide and cisplatin on the proliferation of side population cells from anaplastic thyroid cancer cell lines.

Oncology letters

Li Z,Jiang X,Chen P,Wu X,Duan A,Qin Y

MA5-13854 was used in Western Blotting to suggest that combined treatment with octreotide and cisplatin induces anaplastic thyroid cancer cell apoptosis and suppresses cell proliferation.

Sat Sep 01 00:00:00 UTC 2018

The Human Blood-Nerve Barrier Transcriptome.

Scientific reports

Palladino SP,Helton ES,Jain P,Dong C,Crowley MR,Crossman DK,Ubogu EE

MA5-13854 was used in Immunocytochemistry-immunoflourescence to sequence the human blood-nerve barrier transcriptome using confluent P3 and P8 pHEndEC monolayers and normal adult laser-capture microdissected endoneurial microvessels.

Tue Dec 12 00:00:00 UTC 2017

Analysis of Cancer-Targeting Alkylphosphocholine Analogue Permeability Characteristics Using a Human Induced Pluripotent Stem Cell Blood-Brain Barrier Model.

Molecular pharmaceutics

Clark PA,Al-Ahmad AJ,Qian T,Zhang RR,Wilson HK,Weichert JP,Palecek SP,Kuo JS,Shusta EV

MA5-13854 was used in immunocytochemistry to examine trafficking of cancer-targeting alkylphosphocholine analogues across the blood brain barrier

Tue Sep 06 00:00:00 UTC 2016

Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells.

Methods (San Diego, Calif.)

Stebbins MJ,Wilson HK,Canfield SG,Qian T,Palecek SP,Shusta EV

MA5-13854 was used in immunocytochemistry to describe methods to differentiate and characterize hPSC-derived brain microvascular endothelial cells

Sun May 15 00:00:00 UTC 2016

Influence of P-glycoprotein on brucine transport at the in vitro blood-brain barrier.

European journal of pharmacology

Xu DH,Yan M,Li HD,Fang PF,Liu YW

MA5-13854 was used in immunocytochemistry to study the ability of P-glycoprotein to prevent the transport of brucine through an in vitro blood-brain barrier

Wed Sep 05 00:00:00 UTC 2012

ABCB1 is predominantly expressed in human fetal neural stem/progenitor cells at an early development stage.

Journal of neuroscience research

Yamamoto A,Shofuda T,Islam MO,Nakamura Y,Yamasaki M,Okano H,Kanemura Y

MA5-13854 was used in immunocytochemistry and immunohistochemistry to study the expression of the ABCB1 transporter in human fetal neural stem/progenitor cells

Tue Sep 01 00:00:00 UTC 2009

[The effect of resistance-related proteins on the prognosis and survival of patients with osteosarcoma: an immunohistochemical analysis].

Acta orthopaedica et traumatologica turcica

Ozger H,Eralp L,Atalar AC,Toker B,Esberk Ateş L,Sungur M,Bilgiç B,Ayan I

MA5-13854 was used in immunohistochemistry to examine the prognostic significance of multiple genes in osteosarcoma

Tue May 26 00:00:00 UTC 2009

Efficacy of Hsp90 inhibition for induction of apoptosis and inhibition of growth in cervical carcinoma cells in vitro and in vivo.

Cancer chemotherapy and pharmacology

Schwock J,Pham NA,Cao MP,Hedley DW

MA5-13854 was used in western blot to study the effect of Hsp90 inhibitors on cervical carcinoma survival and growth in vitro and in vivo

Tue Apr 01 00:00:00 UTC 2008

Effect of the novel antipsychotic drug perospirone on P-glycoprotein function and expression in Caco-2 cells.

European journal of clinical pharmacology

Zhou YG,Li KY,Li HD

MA5-13854 was used in immunocytochemistry to study the effects of the novel anti-psychotic drug perospirane of the expression and pump activity of P-glycoprotein

Tue Jul 01 00:00:00 UTC 2008

ABCG2 overexpression in colon cancer cells resistant to SN38 and in irinotecan-treated metastases.

International journal of cancer

Candeil L,Gourdier I,Peyron D,Vezzio N,Copois V,Bibeau F,Orsetti B,Scheffer GL,Ychou M,Khan QA,Pommier Y,Pau B,Martineau P,Del Rio M

MA5-13854 was used in western blot to study the role of ABCG2 overexpression in the development of irinotecan resistance in colon cancer cells

Mon May 10 00:00:00 UTC 2004

Reversal of P-glycoprotein-mediated multidrug resistance with small interference RNA (siRNA) in leukemia cells.

Cancer gene therapy

Peng Z,Xiao Z,Wang Y,Liu P,Cai Y,Lu S,Feng W,Han ZC

MA5-13854 was used in western blot to study the ability of siRNA targeted against P-glycoprotein to reverse multi-drug resistance in a human leukemia cell line

Mon Nov 01 00:00:00 UTC 2004

Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line.

World journal of gastroenterology

Guan J,Chen XP,Zhu H,Luo SF,Cao B,Ding L

MA5-13854 was used in western blot to investigate the role of HBx protein on the occurrence of multidrug resistance in hepatoma cell line

Wed Dec 01 00:00:00 UTC 2004

P-glycoprotein expression and distribution in the rat placenta during pregnancy.

Reproductive toxicology (Elmsford, N.Y.)

Novotna M,Libra A,Kopecky M,Pavek P,Fendrich Z,Semecky V,Staud F

MA5-13854 was used in western blot to study the expression and distribution of P-glycoprotein in the rat placenta during pregnancy

Wed Jan 26 00:00:00 UTC 2005

Differences in multidrug resistance phenotype and matrix metalloproteinases activity between endothelial cells from normal brain and glioma.

Journal of neurochemistry

Régina A,Demeule M,Bérubé A,Moumdjian R,Berthelet F,Béliveau R

MA5-13854 was used in western blot to study differences in the expression of multidrug resistance pumps and MMP activity in endothelial cells from normal brain and glioma

Wed Jan 01 00:00:00 UTC 2003

Examination of the functional activity of P-glycoprotein in the rat placental barrier using rhodamine 123.

The Journal of pharmacology and experimental therapeutics

Pavek P,Staud F,Fendrich Z,Sklenarova H,Libra A,Novotna M,Kopecky M,Nobilis M,Semecky V

MA5-13854 was used in western blot to study the functional activity of P-glycoprotein in the rat placental barrier

Sun Jun 01 00:00:00 UTC 2003

Dexamethasone modulation of multidrug transporters in normal tissues.

FEBS letters

Demeule M,Jodoin J,Beaulieu E,Brossard M,Béliveau R

MA5-13854 was used in western blot to study the effect of dexamethasone on multidrug drug transporter expression in normal rat tissues

Fri Jan 15 00:00:00 UTC 1999

Bioinformatics

Protein Aliases: ATP-binding cassette sub-family B member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-dependent translocase ABCB1; CD243; colchicin sensitivity; doxorubicin resistance; Multidrug resistance protein 1; Multidrug resistance protein 2; P-glycoprotein 1; P-glycoprotein 2; Phospholipid transporter ABCB1

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Gene Aliases: ABC20; ABCB1; CD243; CLCS; GP170; MDR1; P-GP; PGP1; PGP2; PGY1; PGY2

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UniProt ID: (Human) P08183, (Chinese hamster) P21448, (Chinese hamster) P21449

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Entrez Gene ID: (Human) 5243, (Chinese hamster) 100682536, (Chinese hamster) 100682537

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Function(s)

transporter activity protein binding ATP binding xenobiotic-transporting ATPase activity ATPase activity, coupled to transmembrane movement of substances

Process(es)

G2/M transition of mitotic cell cycle antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent positive regulation of antigen processing and presentation of peptide antigen via MHC class I transport drug transmembrane transport response to drug xenobiotic transport transmembrane transport stem cell proliferation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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P-Glycoprotein Monoclonal Antibody (F4)

P-Glycoprotein Antibody (MA5-13854) in ICC/IF

Product Details

MA5-13854

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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