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          • Primary Antibodies ›
          • P2X7 Antibodies

          Alomone Labs, LTD

          P2X7 Receptor (extracellular) Polyclonal Antibody, Atto™ 633

          View all (28) P2X7 antibodies

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          Datasheet
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          Cite P2X7 Receptor (extracellular) Polyclonal Antibody, Atto™ 633

          • Antibody Testing Data (2)
          P2X7 Receptor (extracellular) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          P2X7 Receptor (extracellular) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          P2X7 Receptor (extracellular) Antibody (APR-008-FR-50UL) in ICC/IF

          Expression of P2RX7 in rat brain glioma (C6) cells - Cell surface detection of P2RX7 in intact living rat C6 cells. A. Extracellular staining of cells with Anti-P2X7 Receptor (extracellular)-ATTO Fluor-633 Antibody (#APR-008-FR), (1:25, purple). B. Merged image of A and nuclear staining using DAPI as the counterstain (blue). C. Merged image of B with cells live image. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          P2X7 Receptor (extracellular) Antibody in Immunocytochemistry (ICC/IF)
          P2X7 Receptor (extracellular) Antibody in Flow Cytometry (Flow)

          Product Details

          APR-008-FR-50UL

          Applications
          Tested Dilution
          Publications

          Immunocytochemistry (ICC/IF)

          1:25
          -

          Flow Cytometry (Flow)

          10 µg/1x10^6 cells
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor, Extracellular loop
          View immunogen

          Conjugate

          Atto™ 633 Atto™ 633 Atto™ 633

          Excitation/Emission Max

          630/655 nm

          Form

          Lyophilized

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 1% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Reconstitution: 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).

          Target Information

          The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay criteria.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: ATP receptor; MGC20089; P2X purinoceptor 7; p2X receptor 7; P2X7; P2X7 purinoceptor; P2X7 receptor; P2Z; P2Z receptor; Purinergic receptor; purinergic receptor P2X, ligand gated ion channel, 7; purinergic receptor P2X, ligand-gated ion channel, 7; purinergic receptor P2X7; purinergic receptor P2X7 variant A

          View more View less

          Gene Aliases: AI467586; P2RX7; P2X(7); P2X7; P2X7R

          View more View less

          UniProt ID: (Human) Q99572, (Rat) Q64663, (Mouse) Q9Z1M0

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          Entrez Gene ID: (Human) 5027, (Rat) 29665, (Mouse) 18439

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          Function(s)
          lipopolysaccharide binding purinergic nucleotide receptor activity extracellular ATP-gated cation channel activity receptor binding protein binding ATP binding protein homodimerization activity magnesium ion binding receptor activity copper ion binding zinc ion binding channel activity protein serine/threonine kinase activator activity scaffold protein binding ion channel activity
          Process(es)
          activation of MAPK activity cell morphogenesis phagolysosome assembly positive regulation of T cell mediated cytotoxicity positive regulation of protein phosphorylation regulation of sodium ion transport protein phosphorylation membrane protein ectodomain proteolysis phospholipid transfer to membrane cation transport calcium ion transport cell volume homeostasis membrane budding inflammatory response mitochondrion organization plasma membrane organization cell surface receptor signaling pathway response to mechanical stimulus response to bacterium response to organic substance response to zinc ion gene expression positive regulation of calcium ion transport into cytosol programmed cell death positive regulation of glutamate secretion positive regulation of gamma-aminobutyric acid secretion response to organic cyclic compound organic cation transport synaptic vesicle exocytosis protein processing neuronal action potential sensory perception of pain cytolysis positive regulation of bone mineralization cellular response to extracellular stimulus bleb assembly positive regulation of prostaglandin secretion response to lipopolysaccharide positive regulation of interleukin-1 beta production positive regulation of interleukin-6 production collagen metabolic process response to ATP response to fluid shear stress purinergic nucleotide receptor signaling pathway T cell proliferation response to drug T cell homeostasis positive regulation of apoptotic process positive regulation of catalytic activity NAD transport negative regulation of MAPK cascade positive regulation of MAPK cascade multicellular organismal protein catabolic process phospholipid translocation positive regulation of ossification negative regulation of bone resorption positive regulation of cytolysis ceramide biosynthetic process pore complex assembly skeletal system morphogenesis homeostasis of number of cells within a tissue positive regulation of protein secretion positive regulation of cytokine secretion positive regulation of interleukin-1 alpha secretion positive regulation of interleukin-1 beta secretion defense response to Gram-positive bacterium release of sequestered calcium ion into cytosol protein oligomerization positive regulation of cytoskeleton organization response to calcium ion response to electrical stimulus membrane depolarization positive regulation of mitochondrial depolarization positive regulation of lymphocyte apoptotic process cellular response to organic cyclic compound reactive oxygen species metabolic process apoptotic signaling pathway extrinsic apoptotic signaling pathway cation transmembrane transport transport ion transport positive regulation of gene expression phospholipid scrambling positive regulation of ion transmembrane transport negative regulation of cell volume positive regulation of glycolytic process cellular response to dsRNA positive regulation of bleb assembly blood coagulation regulation of killing of cells of other organism
          It has to be done as per old AB suggested Products section.
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