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P2Y14 (extracellular) Antibody (APR-018-F-50UL) in Flow
Cell surface detection of P2Y14 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + Rabbit IgG Isotype Control-FITC. (green) Cells + Anti-P2Y14 (extracellular)-FITC Antibody (#APR-018-F), (5 µg).
Product Details
APR-018-F-50UL
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CIELKSELGRKWHKASN, corresponding to amino acid residues 172-188 of human P2RY14, Extracellular, 2nd loop
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 15 µL or 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
GPR105 has been reported to be expressed in brain. ESTs have been isolated from kidney, placenta, uterus, and heart/melanocyte/uterus libraries. G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein coupled receptor for UDP-glucose; G protein-coupled receptor 105; G protein-coupled receptor VTR 15-20; G-protein coupled receptor 105; GPCR105; P2Y purinoceptor 14; P2Y(14) receptor; P2Y14; P2Y14 receptor; purinergic receptor P2Y, G-protein coupled, 14; UDP-glucose receptor; VTR 15-20; VTR 15-20 receptor
Gene Aliases: A330108O13Rik; BPR105; GPR105; KIAA0001; P2RY14; P2Y14
UniProt ID: (Human) Q15391, (Mouse) Q9ESG6, (Rat) O35881
Entrez Gene ID: (Human) 9934, (Mouse) 140795, (Rat) 171108
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