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P4HA1 Polyclonal Antibody

P4HA1 Antibody in Immunocytochemistry (ICC/IF)

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P4HA1 Antibody (12658-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed A431 cells using 12658-1-AP (P4HA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

P4HA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

P4HA1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

12658-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

P4HA1 Fusion Protein Ag3292 (236-534 aa encoded by BC034998)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: PEHQRANGN LKYFEYIMAK EKDVNKSASD DQSDQKTTPK KKGVAVDYLP ERQKYEMLCR GEGIKMTPRR QKKLFCRYHD GNRNPKFILA PAKQEDEWDK PRIIRFHDII SDAEIEIVKD LAKPRLRRAT ISNPITGDLE TVHYRISKSA WLSGYENPVV SRINMRIQDL TGLDVSTAEE LQVANYGVGG QYEPHFDFAR KDEPDAFKEL GTGNRIATWL FYMSDVSAGG ATVFPEVGAS VWPKKGTAVF WYNLFASGEG DYSTRHAACP VLVGNKWVSN KWLHERGQEF RRPCTLSELE (236-534 aa encoded by BC034998)

Target Information

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation.

Cell death and differentiation

Chen G,Tang Q,Yu S,Shen Y,Sun J,Peng J,Yin Y,Feng G,Lu X,Mei G,Zhang Y,Wan Q,Zhang L,Chen L

Thu Jun 01 00:00:00 UTC 2023

P4HA1 activates HMGCS1 to promote nasopharyngeal carcinoma ferroptosis resistance and progression.

Cellular signalling

Zhou R,Qiu L,Zhou L,Geng R,Yang S,Wu J

Mon May 01 00:00:00 UTC 2023

P4HA1 activates HMGCS1 to promote nasopharyngeal carcinoma ferroptosis resistance and progression.

Cellular signalling

Zhou R,Qiu L,Zhou L,Geng R,Yang S,Wu J

Mon May 01 00:00:00 UTC 2023

P4HA1, transcriptionally activated by STAT1, promotes esophageal cancer progression.

Pathology international

Gou W,Yang Y,Shan Q,Xia S,Ma Y

Sat Apr 01 00:00:00 UTC 2023

P4HA1, transcriptionally activated by STAT1, promotes esophageal cancer progression.

Pathology international

Gou W,Yang Y,Shan Q,Xia S,Ma Y

Sat Apr 01 00:00:00 UTC 2023

IL-17-induced HIF1α drives resistance to anti-PD-L1 via fibroblast-mediated immune exclusion.

The Journal of experimental medicine

Chen X,Zhao J,Herjan T,Hong L,Liao Y,Liu C,Vasu K,Wang H,Thompson A,Fox PL,Gastman BR,Li X,Li X

Mon Jun 06 00:00:00 UTC 2022

Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.

Nature

Aviner R,Li KH,Frydman J,Andino R

Sun Aug 01 00:00:00 UTC 2021

P4HA1 Down-Regulation Inhibits Glioma Invasiveness by Promoting M1 Microglia Polarization.

OncoTargets and therapy

Wang Q,Zhang J,Fang S,Wang J,Han X,Liu F,Jin G

Thu Apr 21 00:00:00 UTC 2022

Overexpression of P4HA1 associates with poor prognosis and promotes cell proliferation and metastasis of lung adenocarcinoma.

Journal of Cancer

Ning Y,Zheng H,Zhan Y,Liu S,Yang Y,Zang H,Wen Q,Zhang Y,Fan S

Wed Apr 03 00:00:00 UTC 2024

Overexpression of P4HA1 associates with poor prognosis and promotes cell proliferation and metastasis of lung adenocarcinoma.

Journal of Cancer

Ning Y,Zheng H,Zhan Y,Liu S,Yang Y,Zang H,Wen Q,Zhang Y,Fan S

Wed Apr 03 00:00:00 UTC 2024

P4HA1 as an unfavorable prognostic marker promotes cell migration and invasion of glioblastoma via inducing EMT process under hypoxia microenvironment.

American journal of cancer research

Zhu X,Liu S,Yang X,Wang W,Shao W,Ji T

Sat Feb 13 00:00:00 UTC 2021

P4HA1 as an unfavorable prognostic marker promotes cell migration and invasion of glioblastoma via inducing EMT process under hypoxia microenvironment.

American journal of cancer research

Zhu X,Liu S,Yang X,Wang W,Shao W,Ji T

Sat Feb 13 00:00:00 UTC 2021

Identification of a novel glycolysis-related gene signature for predicting the prognosis of osteosarcoma patients.

Aging

Yang M,Ma X,Wang Z,Zhang T,Hua Y,Cai Z

Wed May 05 00:00:00 UTC 2021

HIF-1α interacts with Kindlin-2 and influences breast cancer elasticity: A study based on shear wave elastography imaging.

Cancer medicine

Xue X,Xue S,Wan W,Li J,Shi H

Wed Jul 01 00:00:00 UTC 2020

Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.

Molecular oncology

Eriksson J,Le Joncour V,Jahkola T,Juteau S,Laakkonen P,Saksela O,Hölttä E

Wed Apr 01 00:00:00 UTC 2020

Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.

Molecular oncology

Eriksson J,Le Joncour V,Jahkola T,Juteau S,Laakkonen P,Saksela O,Hölttä E

Wed Apr 01 00:00:00 UTC 2020

Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.

Molecular oncology

Eriksson J,Le Joncour V,Jahkola T,Juteau S,Laakkonen P,Saksela O,Hölttä E

Wed Apr 01 00:00:00 UTC 2020

MiR-122 inhibits epithelial mesenchymal transition by regulating P4HA1 in ovarian cancer cells.

Cell biology international

Duan Y,Dong Y,Dang R,Hu Z,Yang Y,Hu Y,Cheng J

Thu Nov 01 00:00:00 UTC 2018

MiR-122 inhibits epithelial mesenchymal transition by regulating P4HA1 in ovarian cancer cells.

Cell biology international

Duan Y,Dong Y,Dang R,Hu Z,Yang Y,Hu Y,Cheng J

Thu Nov 01 00:00:00 UTC 2018

P4HA1 mutations cause a unique congenital disorder of connective tissue involving tendon, bone, muscle and the eye.

Human molecular genetics

Zou Y,Donkervoort S,Salo AM,Foley AR,Barnes AM,Hu Y,Makareeva E,Leach ME,Mohassel P,Dastgir J,Deardorff MA,Cohn RD,DiNonno WO,Malfait F,Lek M,Leikin S,Marini JC,Myllyharju J,Bönnemann CG

Thu Jun 15 00:00:00 UTC 2017

MiR-30e suppresses proliferation of hepatoma cells via targeting prolyl 4-hydroxylase subunit alpha-1 (P4HA1) mRNA.

Biochemical and biophysical research communications

Feng G,Shi H,Li J,Yang Z,Fang R,Ye L,Zhang W,Zhang X

Fri Apr 08 00:00:00 UTC 2016

MiR-30e suppresses proliferation of hepatoma cells via targeting prolyl 4-hydroxylase subunit alpha-1 (P4HA1) mRNA.

Biochemical and biophysical research communications

Feng G,Shi H,Li J,Yang Z,Fang R,Ye L,Zhang W,Zhang X

Fri Apr 08 00:00:00 UTC 2016

Extracellular matrix remodeling by bone marrow fibroblast-like cells correlates with disease progression in multiple myeloma.

Journal of proteome research

Slany A,Haudek-Prinz V,Meshcheryakova A,Bileck A,Lamm W,Zielinski C,Gerner C,Drach J

Fri Feb 07 00:00:00 UTC 2014

Extracellular matrix remodeling by bone marrow fibroblast-like cells correlates with disease progression in multiple myeloma.

Journal of proteome research

Slany A,Haudek-Prinz V,Meshcheryakova A,Bileck A,Lamm W,Zielinski C,Gerner C,Drach J

Fri Feb 07 00:00:00 UTC 2014

The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression.

Oncotarget

Chakravarthi BV,Pathi SS,Goswami MT,Cieślik M,Zheng H,Nallasivam S,Arekapudi SR,Jing X,Siddiqui J,Athanikar J,Carskadon SL,Lonigro RJ,Kunju LP,Chinnaiyan AM,Palanisamy N,Varambally S

Sat Aug 30 00:00:00 UTC 2014

The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression.

Oncotarget

Chakravarthi BV,Pathi SS,Goswami MT,Cieślik M,Zheng H,Nallasivam S,Arekapudi SR,Jing X,Siddiqui J,Athanikar J,Carskadon SL,Lonigro RJ,Kunju LP,Chinnaiyan AM,Palanisamy N,Varambally S

Sat Aug 30 00:00:00 UTC 2014

The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression.

Oncotarget

Chakravarthi BV,Pathi SS,Goswami MT,Cieślik M,Zheng H,Nallasivam S,Arekapudi SR,Jing X,Siddiqui J,Athanikar J,Carskadon SL,Lonigro RJ,Kunju LP,Chinnaiyan AM,Palanisamy N,Varambally S

Sat Aug 30 00:00:00 UTC 2014

The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression.

Oncotarget

Chakravarthi BV,Pathi SS,Goswami MT,Cieślik M,Zheng H,Nallasivam S,Arekapudi SR,Jing X,Siddiqui J,Athanikar J,Carskadon SL,Lonigro RJ,Kunju LP,Chinnaiyan AM,Palanisamy N,Varambally S

Sat Aug 30 00:00:00 UTC 2014

The effect of isosaponarin isolated from wasabi leaf on collagen synthesis in human fibroblasts and its underlying mechanism.

Journal of natural medicines

Nagai M,Akita K,Yamada K,Okunishi I

Thu Jul 01 00:00:00 UTC 2010

The effect of isosaponarin isolated from wasabi leaf on collagen synthesis in human fibroblasts and its underlying mechanism.

Journal of natural medicines

Nagai M,Akita K,Yamada K,Okunishi I

Thu Jul 01 00:00:00 UTC 2010

The reverse Warburg effect: aerobic glycolysis in cancer associated fibroblasts and the tumor stroma.

Cell cycle (Georgetown, Tex.)

Pavlides S,Whitaker-Menezes D,Castello-Cros R,Flomenberg N,Witkiewicz AK,Frank PG,Casimiro MC,Wang C,Fortina P,Addya S,Pestell RG,Martinez-Outschoorn UE,Sotgia F,Lisanti MP

Tue Dec 01 00:00:00 UTC 2009

The reverse Warburg effect: aerobic glycolysis in cancer associated fibroblasts and the tumor stroma.

Cell cycle (Georgetown, Tex.)

Pavlides S,Whitaker-Menezes D,Castello-Cros R,Flomenberg N,Witkiewicz AK,Frank PG,Casimiro MC,Wang C,Fortina P,Addya S,Pestell RG,Martinez-Outschoorn UE,Sotgia F,Lisanti MP

Tue Dec 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: 4-PH alpha-1; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; prolyl 4-hydroxylase alpha subunit; Prolyl 4-hydroxylase subunit alpha-1; prolyl 4-hydroxylase, alpha polypeptide I

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Gene Aliases: AL022634; P4HA; P4HA1; PHalphaI

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UniProt ID: (Human) P13674, (Mouse) Q60715, (Rat) P54001

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Entrez Gene ID: (Human) 5033, (Mouse) 18451, (Rat) 64475

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Function(s)

procollagen-proline 4-dioxygenase activity iron ion binding protein binding oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen L-ascorbic acid binding oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors metal ion binding dioxygenase activity

Process(es)

peptidyl-proline hydroxylation to 4-hydroxy-L-proline peptidyl-proline hydroxylation collagen fibril organization oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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P4HA1 Polyclonal Antibody

P4HA1 Antibody (12658-1-AP) in ICC/IF

Product Details

12658-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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