Proteintech

P4HA2 Polyclonal Antibody

View all (13) P4HA2 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (13)

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 13

P4HA2 Antibody (13759-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 13759-1-AP ( P4HA2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

P4HA2 Antibody in Western Blot (WB)

P4HA2 Antibody in Western Blot (WB)

P4HA2 Antibody in Western Blot (WB)

P4HA2 Antibody in Western Blot (WB)

P4HA2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 13759-1-AP ( P4HA2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 13759-1-AP ( P4HA2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer using 13759-1-AP (P4HA2 antibody) at dilution of 1:100 (under 40x lens).

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer using 13759-1-AP (P4HA2 antibody) at dilution of 1:100 (under 10x lens).

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 13759-1-AP (P4HA2 antibody) at dilution of 1:50 (under 10x lens).

P4HA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 13759-1-AP (P4HA2 antibody) at dilution of 1:50 (under 40x lens).

P4HA2 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 13759-1-AP) P4HA2 antibody at dilution of 1:2,000 incubated at room temperature for 1.5 hours.

P4HA2 Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 13759-1-AP (P4HA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

P4HA2 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 13759-1-AP (P4HA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

P4HA2 Antibody in Western Blot (WB) — WB result of P4HA2 antibody (13759-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P4HA2 transfected HeLa cells.

P4HA2 Antibody in Immunoprecipitation (IP) — IP result of anti-P4HA2 (IP:13759-1-AP, 4ug; Detection:13759-1-AP 1:1000) with HeLa cells lysate 1200ug.

Product Details

13759-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 14 publications 14 publications

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

-

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

-

Not applicable (N/A)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

P4HA2 Fusion Protein Ag4714 (184-533 aa encoded by BC035813)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: HTVLWMEQV LKQLDAGEEA TTTKSQVLDY LSYAVFQLGD LHRALELTRR LLSLDPSHER AGGNLRYFEQ LLEEEREKTL TNQTEAELAT PEGIYERPVD YLPERDVYES LCRGEGVKLT PRRQKRLFCR YHHGNRAPQL LIAPFKEEDE WDSPHIVRYY DVMSDEEIER IKEIAKPKLA RATVRDPKTG VLTVASYRVS KSSWLEEDDD PVVARVNRRM QHITGLTVKT AELLQVANYG VGGQYEPHFD FSRRPFDSGL KTEGNRLATF LNYMSDVEAG GATVFPDLGA AIWPKKGTAV FWYNLLRSGE GDYRTRHAAC PVLVGCKWVS NKWFHERGQE FLRPCGSTEV D (184-533 aa encoded by BC035813)

Target Information

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Network pharmacological analysis of corosolic acid reveals P4HA2 inhibits hepatocellular carcinoma progression.

BMC complementary medicine and therapies

Tang FF,Liu L,Tian XT,Li N,Peng YX,Qian CM,Jia TT,Liu JJ,Gao WH,Xu YF

Mon May 29 00:00:00 UTC 2023

P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases.

Hepatology (Baltimore, Md.)

Zhang J,Lyu Z,Li B,You Z,Cui N,Li Y,Li Y,Huang B,Chen R,Chen Y,Peng Y,Fang J,Wang Q,Miao Q,Tang R,Gershwin ME,Lian M,Xiao X,Ma X

Sat Jul 01 00:00:00 UTC 2023

P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases.

Hepatology (Baltimore, Md.)

Zhang J,Lyu Z,Li B,You Z,Cui N,Li Y,Li Y,Huang B,Chen R,Chen Y,Peng Y,Fang J,Wang Q,Miao Q,Tang R,Gershwin ME,Lian M,Xiao X,Ma X

Sat Jul 01 00:00:00 UTC 2023

P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases.

Hepatology (Baltimore, Md.)

Zhang J,Lyu Z,Li B,You Z,Cui N,Li Y,Li Y,Huang B,Chen R,Chen Y,Peng Y,Fang J,Wang Q,Miao Q,Tang R,Gershwin ME,Lian M,Xiao X,Ma X

Sat Jul 01 00:00:00 UTC 2023

P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases.

Hepatology (Baltimore, Md.)

Zhang J,Lyu Z,Li B,You Z,Cui N,Li Y,Li Y,Huang B,Chen R,Chen Y,Peng Y,Fang J,Wang Q,Miao Q,Tang R,Gershwin ME,Lian M,Xiao X,Ma X

Sat Jul 01 00:00:00 UTC 2023

P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases.

Hepatology (Baltimore, Md.)

Zhang J,Lyu Z,Li B,You Z,Cui N,Li Y,Li Y,Huang B,Chen R,Chen Y,Peng Y,Fang J,Wang Q,Miao Q,Tang R,Gershwin ME,Lian M,Xiao X,Ma X

Sat Jul 01 00:00:00 UTC 2023

P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases.

Hepatology (Baltimore, Md.)

Zhang J,Lyu Z,Li B,You Z,Cui N,Li Y,Li Y,Huang B,Chen R,Chen Y,Peng Y,Fang J,Wang Q,Miao Q,Tang R,Gershwin ME,Lian M,Xiao X,Ma X

Sat Jul 01 00:00:00 UTC 2023

CEBPB upregulates P4HA2 to promote the malignant biological behavior in IDH1 wildtype glioma.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wang S,Wu J,Zhao W,Li M,Li S

Sat Apr 01 00:00:00 UTC 2023

P4HA2-mediated HIF-1α stabilization promotes erdafitinib-resistance in FGFR3-alteration bladder cancer.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li X,Li Y,Liu B,Chen L,Lyu F,Zhang P,He Q,Cheng L,Liu C,Song Y,Xing Y

Sat Apr 01 00:00:00 UTC 2023

High expression of prolyl 4-hydroxylase subunit alpha-2 in lung adenocarcinoma indicates poor prognosis.

Clinics (Sao Paulo, Brazil)

Lu XH,Sang D,Zhang YR,Yuan Q

Thu Nov 24 00:00:00 UTC 2022

Generation of a homozygous P4HA2 knockout human embryonic stem cell line (FDCHDPe012-A) by CRISPR/Cas9 system.

Stem cell research

You J,Ma S,Zhang H,Chen L

Sat Oct 01 00:00:00 UTC 2022

Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure.

Scientific reports

Isono T,Matsumoto T,Suzaki M,Kubota S,Kageyama S,Kawauchi A,Wada A

Thu Dec 29 00:00:00 UTC 2022

Melanoblast transcriptome analysis reveals pathways promoting melanoma metastasis.

Nature communications

Marie KL,Sassano A,Yang HH,Michalowski AM,Michael HT,Guo T,Tsai YC,Weissman AM,Lee MP,Jenkins LM,Zaidi MR,Pérez-Guijarro E,Day CP,Ylaya K,Hewitt SM,Patel NL,Arnheiter H,Davis S,Meltzer PS,Merlino G,Mishra PJ

Thu Jan 16 00:00:00 UTC 2020

Epigenetic SMAD3 Repression in Tumor-Associated Fibroblasts Impairs Fibrosis and Response to the Antifibrotic Drug Nintedanib in Lung Squamous Cell Carcinoma.

Cancer research

Ikemori R,Gabasa M,Duch P,Vizoso M,Bragado P,Arshakyan M,Luis IC,Marín A,Morán S,Castro M,Fuster G,Gea-Sorli S,Jauset T,Soucek L,Montuenga LM,Esteller M,Monsó E,Peinado VI,Gascon P,Fillat C,Hilberg F,Reguart N,Alcaraz J

Wed Jan 15 00:00:00 UTC 2020

P4HA2 contributes to cervical cancer progression via inducing epithelial-mesenchymal transition.

Journal of Cancer

Cao Y,Han Q,Li J,Jia Y,Zhang R,Shi H

Mon Sep 28 00:00:00 UTC 2020

P4HA2 contributes to cervical cancer progression via inducing epithelial-mesenchymal transition.

Journal of Cancer

Cao Y,Han Q,Li J,Jia Y,Zhang R,Shi H

Mon Sep 28 00:00:00 UTC 2020

P4HA2 contributes to cervical cancer progression via inducing epithelial-mesenchymal transition.

Journal of Cancer

Cao Y,Han Q,Li J,Jia Y,Zhang R,Shi H

Mon Sep 28 00:00:00 UTC 2020

Collagen prolyl 4-hydroxylase 2 predicts worse prognosis and promotes glycolysis in cervical cancer.

American journal of translational research

Li Q,Wang Q,Zhang Q,Zhang J,Zhang J

Mon Sep 28 00:00:00 UTC 2020

Collagen prolyl 4-hydroxylase 2 predicts worse prognosis and promotes glycolysis in cervical cancer.

American journal of translational research

Li Q,Wang Q,Zhang Q,Zhang J,Zhang J

Mon Sep 28 00:00:00 UTC 2020

Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma.

EBioMedicine

Wang T,Fu X,Jin T,Zhang L,Liu B,Wu Y,Xu F,Wang X,Ye K,Zhang W,Ye L

Mon Jul 01 00:00:00 UTC 2019

Prolyl 4-hydroxylase 2 promotes B-cell lymphoma progression via hydroxylation of Carabin.

Blood

Jiang W,Zhou X,Li Z,Liu K,Wang W,Tan R,Cong X,Shan J,Zhan Y,Cui Z,Jiang L,Li Q,Shen S,Bai M,Cheng Y,Li B,Tan M,Ma DK,Liu JO,Dang Y

Thu Mar 22 00:00:00 UTC 2018

Prolyl 4-hydroxylase 2 promotes B-cell lymphoma progression via hydroxylation of Carabin.

Blood

Jiang W,Zhou X,Li Z,Liu K,Wang W,Tan R,Cong X,Shan J,Zhan Y,Cui Z,Jiang L,Li Q,Shen S,Bai M,Cheng Y,Li B,Tan M,Ma DK,Liu JO,Dang Y

Thu Mar 22 00:00:00 UTC 2018

Nintedanib selectively inhibits the activation and tumour-promoting effects of fibroblasts from lung adenocarcinoma patients.

British journal of cancer

Gabasa M,Ikemori R,Hilberg F,Reguart N,Alcaraz J

Tue Oct 10 00:00:00 UTC 2017

Nintedanib selectively inhibits the activation and tumour-promoting effects of fibroblasts from lung adenocarcinoma patients.

British journal of cancer

Gabasa M,Ikemori R,Hilberg F,Reguart N,Alcaraz J

Tue Oct 10 00:00:00 UTC 2017

Hepatitis B virus X protein promotes the development of liver fibrosis and hepatoma through downregulation of miR-30e targeting P4HA2 mRNA.

Oncogene

Feng GX,Li J,Yang Z,Zhang SQ,Liu YX,Zhang WY,Ye LH,Zhang XD

Thu Dec 14 00:00:00 UTC 2017

Hepatitis B virus X protein promotes the development of liver fibrosis and hepatoma through downregulation of miR-30e targeting P4HA2 mRNA.

Oncogene

Feng GX,Li J,Yang Z,Zhang SQ,Liu YX,Zhang WY,Ye LH,Zhang XD

Thu Dec 14 00:00:00 UTC 2017

Hepatitis B virus X protein promotes the development of liver fibrosis and hepatoma through downregulation of miR-30e targeting P4HA2 mRNA.

Oncogene

Feng GX,Li J,Yang Z,Zhang SQ,Liu YX,Zhang WY,Ye LH,Zhang XD

Thu Dec 14 00:00:00 UTC 2017

Hepatitis B virus X protein promotes the development of liver fibrosis and hepatoma through downregulation of miR-30e targeting P4HA2 mRNA.

Oncogene

Feng GX,Li J,Yang Z,Zhang SQ,Liu YX,Zhang WY,Ye LH,Zhang XD

Thu Dec 14 00:00:00 UTC 2017

Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.

Journal of molecular and cellular cardiology

Cowling RT,Park JI,Sotimehin AE,Greenberg BH

Fri Dec 01 00:00:00 UTC 2017

Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.

Journal of molecular and cellular cardiology

Cowling RT,Park JI,Sotimehin AE,Greenberg BH

Fri Dec 01 00:00:00 UTC 2017

Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.

Journal of molecular and cellular cardiology

Cowling RT,Park JI,Sotimehin AE,Greenberg BH

Fri Dec 01 00:00:00 UTC 2017

Identification of PRDX4 and P4HA2 as metastasis-associated proteins in oral cavity squamous cell carcinoma by comparative tissue proteomics of microdissected specimens using iTRAQ technology.

Journal of proteome research

Chang KP,Yu JS,Chien KY,Lee CW,Liang Y,Liao CT,Yen TC,Lee LY,Huang LL,Liu SC,Chang YS,Chi LM

Fri Nov 04 00:00:00 UTC 2011

Identification of PRDX4 and P4HA2 as metastasis-associated proteins in oral cavity squamous cell carcinoma by comparative tissue proteomics of microdissected specimens using iTRAQ technology.

Journal of proteome research

Chang KP,Yu JS,Chien KY,Lee CW,Liang Y,Liao CT,Yen TC,Lee LY,Huang LL,Liu SC,Chang YS,Chi LM

Fri Nov 04 00:00:00 UTC 2011

Identification of PRDX4 and P4HA2 as metastasis-associated proteins in oral cavity squamous cell carcinoma by comparative tissue proteomics of microdissected specimens using iTRAQ technology.

Journal of proteome research

Chang KP,Yu JS,Chien KY,Lee CW,Liang Y,Liao CT,Yen TC,Lee LY,Huang LL,Liu SC,Chang YS,Chi LM

Fri Nov 04 00:00:00 UTC 2011

Identification of PRDX4 and P4HA2 as metastasis-associated proteins in oral cavity squamous cell carcinoma by comparative tissue proteomics of microdissected specimens using iTRAQ technology.

Journal of proteome research

Chang KP,Yu JS,Chien KY,Lee CW,Liang Y,Liao CT,Yen TC,Lee LY,Huang LL,Liu SC,Chang YS,Chi LM

Fri Nov 04 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: 4-PH alpha 2; 4-PH alpha-2; 4PHalpha2; C-P4Halpha(II); collagen prolyl 4-hydroxylase alpha(II); procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Prolyl 4-hydroxylase subunit alpha-2; prolyl 4-hydroxylase, alpha polypeptide II

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Gene Aliases: P4HA2; UNQ290/PRO330

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UniProt ID: (Human) O15460

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Entrez Gene ID: (Human) 8974

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procollagen-proline 4-dioxygenase activity iron ion binding electron carrier activity oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen L-ascorbic acid binding

peptidyl-proline hydroxylation oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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