Proteintech

P5CS Polyclonal Antibody

P5CS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

P5CS Antibody (17719-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 17719-1-AP) (P5CS antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

P5CS Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17719-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

P5CS Fusion Protein Ag12100 (1-362 aa encoded by BC106054)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MLSQVYRCG FQPFNQHLLP WVKCTTVFRS HCIQPSVIRH VRSWSNIPFI TVPLSRTHGK SFAHRSELKH AKRIVVKLGS AVVTRGDECG LALGRLASIV EQVSVLQNQG REMMLVTSGA VAFGKQRLRH EILLSQSVRQ ALHSGQNQLK EMAIPVLEAR ACAAAGQSGL MALYEAMFTQ YSICAAQILV TNLDFHDEQK RRNLNGTLHE LLRMNIVPIV NTNDAVVPPA EPNSDLQGVN VISVKDNDSL AARLAVEMKT DLLIVLSDVE GLFDSPPGSD DAKLIDIFYP GDQQSVTFGI KSRVGMGGME AKVKAALWAL QGGTSVVIAN GTHPKVSGHV ITDIVEGKKV GTFFSEVKPA GPT (1-362 aa encoded by BC106054)

Target Information

This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine. Mutations in this gene lead to hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia and may be associated with neurodegeneration, cataracts and connective tissue diseases. Alternatively spliced transcript variants, encoding different isoforms, have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Single-Cell Metabolomics-Based Strategy for Studying the Mechanisms of Drug Action.

Analytical chemistry

Zhu G,Zhang W,Zhao Y,Chen T,Yuan H,Liu Y,Guo G,Liu Z,Wang X

Tue Mar 14 00:00:00 UTC 2023

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

EMBO molecular medicine

Southwell N,Primiano G,Nadkarni V,Attarwala N,Beattie E,Miller D,Alam S,Liparulo I,Shurubor YI,Valentino ML,Carelli V,Servidei S,Gross SS,Manfredi G,Chen Q,D'Aurelio M

Mon Jul 10 00:00:00 UTC 2023

Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.

Autophagy

Bai J,Liu T,Tu B,Yuan M,Shu Z,Fan M,Huo S,Guo Y,Wang L,Wang H,Zhao Y

Wed Feb 01 00:00:00 UTC 2023

Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix.

Nature metabolism

Kay EJ,Paterson K,Riera-Domingo C,Sumpton D,Däbritz JHM,Tardito S,Boldrini C,Hernandez-Fernaud JR,Athineos D,Dhayade S,Stepanova E,Gjerga E,Neilson LJ,Lilla S,Hedley A,Koulouras G,McGregor G,Jamieson C,Johnson RM,Park M,Kirschner K,Miller C,Kamphorst JJ,Loayza-Puch F,Saez-Rodriguez J,Mazzone M,Blyth K,Zagnoni M,Zanivan S

Wed Jun 01 00:00:00 UTC 2022

PINCH-1 promotes Δ¹-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.

Amino acids

Cui C,Wang J,Guo L,Wu C

Wed Dec 01 00:00:00 UTC 2021

PINCH-1 promotes Δ¹-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.

Amino acids

Cui C,Wang J,Guo L,Wu C

Wed Dec 01 00:00:00 UTC 2021

Mitochondrial NADP⁺ is essential for proline biosynthesis during cell growth.

Nature metabolism

Tran DH,Kesavan R,Rion H,Soflaee MH,Solmonson A,Bezwada D,Vu HS,Cai F,Phillips JA,DeBerardinis RJ,Hoxhaj G

Thu Apr 01 00:00:00 UTC 2021

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

The Journal of experimental medicine

Liu M,Wang Y,Yang C,Ruan Y,Bai C,Chu Q,Cui Y,Chen C,Ying G,Li B

Mon Mar 02 00:00:00 UTC 2020

Kindlin-2 links mechano-environment to proline synthesis and tumor growth.

Nature communications

Guo L,Cui C,Zhang K,Wang J,Wang Y,Lu Y,Chen K,Yuan J,Xiao G,Tang B,Sun Y,Wu C

Tue Feb 19 00:00:00 UTC 2019

Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.

American journal of respiratory cell and molecular biology

Hamanaka RB,O'Leary EM,Witt LJ,Tian Y,Gökalp GA,Meliton AY,Dulin NO,Mutlu GM

Fri Nov 01 00:00:00 UTC 2019

Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.

Scientific reports

Liu W,Hancock CN,Fischer JW,Harman M,Phang JM

Tue Nov 24 00:00:00 UTC 2015

Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.

Scientific reports

Liu W,Hancock CN,Fischer JW,Harman M,Phang JM

Tue Nov 24 00:00:00 UTC 2015

Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.

Proceedings of the National Academy of Sciences of the United States of America

Liu W,Le A,Hancock C,Lane AN,Dang CV,Fan TW,Phang JM

Tue Jun 05 00:00:00 UTC 2012

Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.

Proceedings of the National Academy of Sciences of the United States of America

Liu W,Le A,Hancock C,Lane AN,Dang CV,Fan TW,Phang JM

Tue Jun 05 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: aldehyde dehydrogenase 18 family, member A1; Aldehyde dehydrogenase family 18 member A1; Delta-1-pyrroline-5-carboxylate synthase; delta-1-pyrroline-5-carboxylate synthetase; delta1-pyrroline-5-carboxlate synthetase; glutamate gamma-semialdehyde synthetase; MGC117316; P5CS; pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase)

View more View less

Gene Aliases: 2810433K04Rik; ADCL3; AI429789; ALDH18A1; ARCL3A; GSAS; P5CS; PYCS; SPG9A; SPG9B

View more View less

UniProt ID: (Human) P54886, (Mouse) Q9Z110

View more View less

Entrez Gene ID: (Human) 5832, (Rat) 361755, (Mouse) 56454

View more View less

Function(s)

glutamate 5-kinase activity glutamate-5-semialdehyde dehydrogenase activity protein binding ATP binding delta1-pyrroline-5-carboxylate synthetase activity identical protein binding poly(A) RNA binding nucleotide binding catalytic activity kinase activity oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor transferase activity

Process(es)

glutamate metabolic process proline biosynthetic process ornithine biosynthetic process phosphorylation citrulline biosynthetic process oxidation-reduction process metabolic process cellular amino acid biosynthetic process L-proline biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

P5CS Polyclonal Antibody

P5CS Antibody (17719-1-AP) in IHC (P)

Product Details

17719-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!