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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SQSTM1 Antibodies

          Proteintech

          P62/SQSTM1 Polyclonal Antibody

          1,353 References
          View all (83) SQSTM1 antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite P62/SQSTM1 Polyclonal Antibody

          • Antibody Testing Data (28)
          P62/SQSTM1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          P62/SQSTM1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 28

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          P62/SQSTM1 Antibody (18420-1-AP) in ICC/IF

          Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using P62,SQSTM1 antibody (Product # 18420-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          P62/SQSTM1 Antibody in Immunocytochemistry (ICC/IF)
          P62/SQSTM1 Antibody in Immunocytochemistry (ICC/IF)
          P62/SQSTM1 Antibody in Immunocytochemistry (ICC/IF)
          P62/SQSTM1 Antibody in Immunocytochemistry (ICC/IF)
          P62/SQSTM1 Antibody in Immunocytochemistry (ICC/IF)
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Western Blot (WB)
          P62/SQSTM1 Antibody in Western Blot (WB)
          P62/SQSTM1 Antibody in Western Blot (WB)
          P62/SQSTM1 Antibody in Western Blot (WB)
          P62/SQSTM1 Antibody in Western Blot (WB)
          P62/SQSTM1 Antibody in Western Blot (WB)
          P62/SQSTM1 Antibody in Western Blot (WB)
          P62/SQSTM1 Antibody in Flow Cytometry (Flow)
          P62/SQSTM1 Antibody in Immunoprecipitation (IP)

          Product Details

          18420-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:4,000
          View 1,062 publications 1,062 publications

          Immunohistochemistry (IHC)

          -
          View 118 publications 118 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          View 134 publications 134 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          -

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 19 publications 19 publications

          Miscellaneous PubMed (MISC)

          -
          View 6 publications 6 publications

          Not applicable (N/A)

          -
          View 14 publications 14 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Chicken, Human, Mouse, Pig, Rabbit, Rat, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          P62/SQSTM1 Fusion Protein Ag13131 (1-440 aa encoded by BC017222)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.29 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          The molecular weight of p62 is predicted to be 48/ 38 kDa (depending on the isoform), while western blot analyses using this antibody detects the bands around 45-48 kDa and 60-62 kDa, respectively.

          Immunogen sequence: MASLTVKAY LLGKEDAARE IRRFSFCCSP EPEAEAEAAA GPGPCERLLS RVAALFPALR PGGFQAHYRD EDGDLVAFSS DEELTMAMSY VKDDIFRIYI KEKKECRRDH RPPCAQEAPR NMVHPNVICD GCNGPVVGTR YKCSVCPDYD LCSVCEGKGL HRGHTKLAFP SPFGHLSEGF SHSRWLRKVK HGHFGWPGWE MGPPGNWSPR PPRAGEARPG PTAESASGPS EDPSVNFLKN VGESVAAALS PLGIEVDIDV EHGGKRSRLT PVSPESSSTE EKSSSQPSSC CSDPSKPGGN VEGATQSLAE QMRKIALESE GRPEEQMESD NCSGGDDDWT HLSSKEVDPS TGELQSLQMP ESEGPSSLDP SQEGPTGLKE AALYPHLPPE ADPRLIESLS QMLSMGFSDE GGWLTRLLQT KNYDIGAALD TIQYSKHPPP L (1-440 aa encoded by BC017222)

          Target Information

          This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; oxidative stress induced; oxidative stress induced like; p62; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; PKC-zeta-interacting protein; Protein kinase C-zeta-interacting protein; Sequestosome-1; STONE14; Ubiquitin-binding protein p62

          View more View less

          Gene Aliases: A170; FTDALS3; ORCA; OSF-6; Osi; OSIL; p60; p62; p62B; PDB3; SQSTM1; STAP; STONE14; ZIP; ZIP3

          View more View less

          UniProt ID: (Human) Q13501, (Rat) O08623, (Mouse) Q64337

          View more View less

          Entrez Gene ID: (Human) 8878, (Rat) 113894, (Mouse) 18412

          View more View less

          Function(s)
          protein serine/threonine kinase activity protein kinase C binding protein binding zinc ion binding protein kinase binding receptor tyrosine kinase binding ubiquitin protein ligase binding SH2 domain binding identical protein binding protein homodimerization activity ubiquitin binding K63-linked polyubiquitin binding transcription cofactor activity metal ion binding
          Process(es)
          positive regulation of protein phosphorylation immune system process ubiquitin-dependent protein catabolic process autophagy apoptotic process macroautophagy positive regulation of macroautophagy cell differentiation regulation of I-kappaB kinase/NF-kappaB signaling protein heterooligomerization regulation of mitophagy mitophagy protein phosphorylation response to stress protein localization regulation of mitochondrion organization endosomal transport intracellular signal transduction positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of growth of symbiont in host positive regulation of transcription from RNA polymerase II promoter regulation of Ras protein signal transduction regulation of protein complex stability mitophagy in response to mitochondrial depolarization regulation of nucleic acid-templated transcription
          It has to be done as per old AB suggested Products section.
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