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          • Primary Antibodies ›
          • SQSTM1 Antibodies

          Proteintech

          P62/SQSTM1 Monoclonal Antibody (1H5C1)

          100 References
          View all (83) SQSTM1 antibodies

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          Cite P62/SQSTM1 Monoclonal Antibody (1H5C1)

          • Antibody Testing Data (10)
          P62/SQSTM1 Antibody in Immunocytochemistry (ICC/IF)
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          P62/SQSTM1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          P62/SQSTM1 Antibody (66184-1-IG) in ICC/IF

          Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using P62/SQSTM1 antibody (Product # 66184-1-Ig) (Clone: 1H5C1) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          P62/SQSTM1 Antibody in Immunocytochemistry (ICC/IF)
          P62/SQSTM1 Antibody in Immunocytochemistry (ICC/IF)
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          P62/SQSTM1 Antibody in Western Blot (WB)
          P62/SQSTM1 Antibody in Western Blot (WB)
          P62/SQSTM1 Antibody in Western Blot (WB)
          P62/SQSTM1 Antibody in Western Blot (WB)
          P62/SQSTM1 Antibody in Flow Cytometry (Flow)

          Product Details

          66184-1-IG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:4,000
          View 81 publications 81 publications

          Immunohistochemistry (IHC)

          -
          View 7 publications 7 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          View 9 publications 9 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          -

          Immunoprecipitation (IP)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Mouse / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1H5C1

          Immunogen

          P62/SQSTM1 Fusion Protein Ag13131 (1-440 aa encoded by BC017222)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • CoraLite 594
          • CoraLite Plus 488
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1.33 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.

          Immunogen sequence: MASLTVKAY LLGKEDAARE IRRFSFCCSP EPEAEAEAAA GPGPCERLLS RVAALFPALR PGGFQAHYRD EDGDLVAFSS DEELTMAMSY VKDDIFRIYI KEKKECRRDH RPPCAQEAPR NMVHPNVICD GCNGPVVGTR YKCSVCPDYD LCSVCEGKGL HRGHTKLAFP SPFGHLSEGF SHSRWLRKVK HGHFGWPGWE MGPPGNWSPR PPRAGEARPG PTAESASGPS EDPSVNFLKN VGESVAAALS PLGIEVDIDV EHGGKRSRLT PVSPESSSTE EKSSSQPSSC CSDPSKPGGN VEGATQSLAE QMRKIALESE GRPEEQMESD NCSGGDDDWT HLSSKEVDPS TGELQSLQMP ESEGPSSLDP SQEGPTGLKE AALYPHLPPE ADPRLIESLS QMLSMGFSDE GGWLTRLLQT KNYDIGAALD TIQYSKHPPP L (1-440 aa encoded by BC017222)

          Target Information

          This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; oxidative stress induced like; p62; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Sequestosome-1; Ubiquitin-binding protein p62

          View more View less

          Gene Aliases: A170; FTDALS3; ORCA; OSIL; p60; p62; p62B; PDB3; SQSTM1; ZIP3

          View more View less

          UniProt ID: (Human) Q13501

          View more View less

          Entrez Gene ID: (Human) 8878

          View more View less

          Function(s)
          protein serine/threonine kinase activity protein kinase C binding protein binding zinc ion binding protein kinase binding receptor tyrosine kinase binding ubiquitin protein ligase binding SH2 domain binding identical protein binding protein homodimerization activity ubiquitin binding K63-linked polyubiquitin binding
          Process(es)
          mitophagy positive regulation of protein phosphorylation immune system process protein phosphorylation ubiquitin-dependent protein catabolic process autophagy apoptotic process response to stress protein localization regulation of mitochondrion organization endosomal transport macroautophagy positive regulation of macroautophagy cell differentiation intracellular signal transduction positive regulation of apoptotic process negative regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of growth of symbiont in host positive regulation of transcription from RNA polymerase II promoter regulation of Ras protein signal transduction protein heterooligomerization regulation of protein complex stability mitophagy in response to mitochondrial depolarization
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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