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PABP4 Antibody (A301-466A) in IHC (P)
Detection of human PABP4 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti-PABP4 (Product # A301-466A) (Lot 1) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.
Product Details
A301-466A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 525 and 575 of human Poly(A)-binding protein 4.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activated-platelet protein 1; APP-1; Inducible poly(A)-binding protein; iPABP; PABP-4; poly(A) binding protein, cytoplasmic 4 (inducible form); poly(A)-binding protein 4; Polyadenylate-binding protein 4
Gene Aliases: APP-1; APP1; iPABP; PABP4; PABPC4
UniProt ID: (Human) Q13310
Entrez Gene ID: (Human) 8761, (Mouse) 230721
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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