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PACSIN3 Antibody (PA5-97139) in WB
Western blot analysis of PACSIN3 in extracts of various cell lines. Samples were incubated with PACSIN3 Polyclonal antibody (Product # PA5-97139) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-97139
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 175-424 of human PACSIN3 (NP_0573072)
Conjugate
Form
Liquid
Concentration
1.16 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808941
Product Specific Information
Immunogen sequence: KADSAVSQEQ LRKLQERVER CAKEAEKTKA QYEQTLAELH RYTPRYMEDM EQAFETCQAA ERQRLLFFKD MLLTLHQHLD LSSSEKFHEL HRDLHQGIEA ASDEEDLRWW RSTHGPGMAM NWPQFEEWSL DTQRTISRKE KGGRSPDEVT LTSIVPTRDG TAPPPQSPGS PGTGQDEEWS DEESPRKAAT GVRVRALYDY AGQEADELSF RAGEELLKMS EEDEQGWCQG QLQSGRIGLY PANYVECVGA; Positive Samples: HepG2, MCF-7, HeLa, Jurkat, Mouse heart, Mouse liver, Mouse lung, Rat liver, Rat brain; Cellular Location: Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein
Target Information
This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Endophilin I; Protein kinase C and casein kinase II substrate protein 3; Protein kinase C and casein kinase substrate in neurons protein 3; SH3 domain-containing protein 6511; syndapin III
Gene Aliases: 4921507A02Rik; 6330413E15Rik; AW413130; PACSIN3; SDPIII
UniProt ID: (Human) Q9UKS6, (Mouse) Q99JB8
Entrez Gene ID: (Human) 29763, (Rat) 311187, (Mouse) 80708
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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