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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PACT Antibodies

          Invitrogen

          PACT Recombinant Rabbit Monoclonal Antibody (2W2E10)

          Advanced Verification
          View all (14) PACT antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite PACT Recombinant Rabbit Monoclonal Antibody (2W2E10)

          • Antibody Testing Data (5)
          • Advanced Verification (1)
          PACT Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          PACT Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          PACT Antibody (MA5-35717) in IHC (P)

          Immunohistochemistry analysis of PACT in paraffin-embedded mouse spinal cord. Samples were incubated with PACT Monoclonal antibody (Product # MA5-35717) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PACT Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PACT Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PACT Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PACT Antibody in Western Blot (WB)
          PACT Antibody in Western Blot (WB)
          PACT Antibody

          Product Details

          MA5-35717

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:500
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          2W2E10

          Immunogen

          A synthetic peptide corresponding to a sequence within amino acids 214-313 of human PRKRA/PACT (O75569)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.15 mg/mL

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol, 0.05% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2849617

          Product Specific Information

          Immunogen sequence: TWHSLRNSPG EKINLLKRSL LSIPNTDYIQ LLSEIAKEQG FNITYLDIDE LSANGQYQCL AELSTSPITV CHGSGISCGN AQSDAAHNAL QYLKIIAERK

          Target Information

          PRKRA appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. It activates EIF2AK2 in the absence of double-stranded RNA (dsRNA).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: interferon-inducible double stranded RNA-dependent protein kinase activator A; Interferon-inducible double-stranded RNA-dependent protein kinase activator A; PKR associated protein X; PKR-associated protein X; PKR-associating protein X; Protein activator of the interferon-induced protein kinase; protein kinase, interferon-inducible double stranded RNA dependent activator; protein kinase, interferon-inducible double stranded RNA-dependent activator; Protein kinase, interferon-inducible double-stranded RNA-dependent activator; RAX

          View more View less

          Gene Aliases: AV120107; DYT16; HSD-14; HSD14; lear; PACT; PRK; PRKRA; RAX

          View more View less

          UniProt ID: (Human) O75569, (Rat) Q4V8C7, (Mouse) Q9WTX2

          View more View less

          Entrez Gene ID: (Human) 8575, (Rat) 311130, (Mouse) 23992

          View more View less

          Function(s)
          double-stranded RNA binding protein binding enzyme activator activity enzyme binding identical protein binding protein homodimerization activity poly(A) RNA binding RNA binding kinase activity protein kinase binding
          Process(es)
          protein phosphorylation positive regulation of cell proliferation production of siRNA involved in RNA interference cellular response to oxidative stress outer ear morphogenesis middle ear morphogenesis skeletal system morphogenesis positive regulation of intrinsic apoptotic signaling pathway immune response negative regulation of cell proliferation response to virus gene silencing by RNA pre-miRNA processing production of miRNAs involved in gene silencing by miRNA positive regulation of catalytic activity
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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