Proteintech

PADI2 Polyclonal Antibody

PADI2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 22

PADI2 Antibody (12110-1-AP) in ICC/IF

Immunofluorescent analysis of BxPC-3 cells using 12110-1-AP (PADI2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PADI2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PADI2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

PADI2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

12110-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 34 publications 34 publications

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:10-1:100

Immunocytochemistry (ICC/IF)

1:10-1:100

View 18 publications 18 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Not applicable (N/A)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PADI2 Fusion Protein Ag2755 (118-438 aa encoded by BC009701)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ISLDVDADR DGVVEKNNPK KASWTWGPEG QGAILLVNCD RETPWLPKED CRDEKVYSKE DLKDMSQMIL RTKGPDRLPA GYEIVLYISM SDSDKVGVFY VENPFFGQRY IHILGRRKLY HVVKYTGGSA ELLFFVEGLC FPDEGFSGLV SIHVSLLEYM AQDIPLTPIF TDTVIFRIAP WIMTPNILPP VSVFVCCMKD NYLFLKEVKN LVEKTNCELK VCFQYLNRGD RWIQDEIEFG YIEAPHKGFP VVLDSPRDGN LKDFPVKELL GPDFGYVTRE PLFESVTSLD SFGNLEVSPP VTVNGKTYPL GRILIGSSFP L (118-438 aa encoded by BC009701)

Target Information

This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in a cluster with four other paralogous genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in mice.

Oncology reports

Kim S,Song YK,Cho CS,Kim HJ,Fang S,Jo DH,Kim H

Sat Jul 01 00:00:00 UTC 2023

Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in mice.

Oncology reports

Kim S,Song YK,Cho CS,Kim HJ,Fang S,Jo DH,Kim H

Sat Jul 01 00:00:00 UTC 2023

PAD-2-mediated citrullination of nucleophosmin provides an effective target for tumor immunotherapy.

Journal for immunotherapy of cancer

Choudhury RH,Symonds P,Paston SJ,Daniels I,Cook KW,Gijon M,Metheringham RL,Brentville VA,Durrant LG

Tue Feb 01 00:00:00 UTC 2022

PAD-2-mediated citrullination of nucleophosmin provides an effective target for tumor immunotherapy.

Journal for immunotherapy of cancer

Choudhury RH,Symonds P,Paston SJ,Daniels I,Cook KW,Gijon M,Metheringham RL,Brentville VA,Durrant LG

Tue Feb 01 00:00:00 UTC 2022

IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation.

The Journal of investigative dermatology

Padhi A,Srivastava A,Ramesh A,Ehrström M,Simon M,Sonkoly E,Eidsmo L,Bergman P,Lysell J

Tue Feb 01 00:00:00 UTC 2022

Delayed puberty, gonadotropin abnormalities and subfertility in male Padi2/Padi4 double knockout mice.

Reproductive biology and endocrinology : RB&E

Sams KL,Mukai C,Marks BA,Mittal C,Demeter EA,Nelissen S,Grenier JK,Tate AE,Ahmed F,Coonrod SA

Wed Oct 12 00:00:00 UTC 2022

Identification of super-enhancer-driven peptidyl arginine deiminases as potential biomarkers and therapeutic targets for osimertinib-resistant non-small cell lung cancer.

Frontiers in pharmacology

Li H,Muhetaer G,Xie Y,Yao K,Ma Q,Guan H,Xing S,Huang X,Zhou J

Sat Dec 10 00:00:00 UTC 2022

Peptidylarginine deiminase enzymes and citrullinated proteins in female reproductive physiology and associated diseases†.

Biology of reproduction

Christensen AO,Li G,Young CH,Snow B,Khan SA,DeVore SB,Edwards S,Bouma GJ,Navratil AM,Cherrington BD,Rothfuss HM

Sat Dec 10 00:00:00 UTC 2022

Peptidylarginine deiminase enzymes and citrullinated proteins in female reproductive physiology and associated diseases†.

Biology of reproduction

Christensen AO,Li G,Young CH,Snow B,Khan SA,DeVore SB,Edwards S,Bouma GJ,Navratil AM,Cherrington BD,Rothfuss HM

Sat Dec 10 00:00:00 UTC 2022

Peptidylarginine deiminase enzymes and citrullinated proteins in female reproductive physiology and associated diseases†.

Biology of reproduction

Christensen AO,Li G,Young CH,Snow B,Khan SA,DeVore SB,Edwards S,Bouma GJ,Navratil AM,Cherrington BD,Rothfuss HM

Sat Dec 10 00:00:00 UTC 2022

Peptidylarginine deiminase enzymes and citrullinated proteins in female reproductive physiology and associated diseases†.

Biology of reproduction

Christensen AO,Li G,Young CH,Snow B,Khan SA,DeVore SB,Edwards S,Bouma GJ,Navratil AM,Cherrington BD,Rothfuss HM

Sat Dec 10 00:00:00 UTC 2022

Peptidylarginine deiminase enzymes and citrullinated proteins in female reproductive physiology and associated diseases†.

Biology of reproduction

Christensen AO,Li G,Young CH,Snow B,Khan SA,DeVore SB,Edwards S,Bouma GJ,Navratil AM,Cherrington BD,Rothfuss HM

Sat Dec 10 00:00:00 UTC 2022

Peptidylarginine deiminase enzymes and citrullinated proteins in female reproductive physiology and associated diseases†.

Biology of reproduction

Christensen AO,Li G,Young CH,Snow B,Khan SA,DeVore SB,Edwards S,Bouma GJ,Navratil AM,Cherrington BD,Rothfuss HM

Sat Dec 10 00:00:00 UTC 2022

Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.

eLife

Gargareta VI,Reuschenbach J,Siems SB,Sun T,Piepkorn L,Mangana C,Späte E,Goebbels S,Huitinga I,Möbius W,Nave KA,Jahn O,Werner HB

Wed May 11 00:00:00 UTC 2022

Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes.

Angiogenesis

Bai J,Khajavi M,Sui L,Fu H,Tarakkad Krishnaji S,Birsner AE,Bazinet L,Kamm RD,D'Amato RJ

Mon Feb 01 00:00:00 UTC 2021

Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes.

Angiogenesis

Bai J,Khajavi M,Sui L,Fu H,Tarakkad Krishnaji S,Birsner AE,Bazinet L,Kamm RD,D'Amato RJ

Mon Feb 01 00:00:00 UTC 2021

Progesterone stimulates histone citrullination to increase IGFBP1 expression in uterine cells.

Reproduction (Cambridge, England)

Young CH,Snow B,DeVore SB,Mohandass A,Nemmara VV,Thompson PR,Thyagarajan B,Navratil AM,Cherrington BD

Thu Jul 08 00:00:00 UTC 2021

Peptidylarginine deiminase 2 has potential as both a biomarker and therapeutic target of sepsis.

JCI insight

Tian Y,Qu S,Alam HB,Williams AM,Wu Z,Deng Q,Pan B,Zhou J,Liu B,Duan X,Ma J,Mondal S,Thompson PR,Stringer KA,Standiford TJ,Li Y

Thu Oct 15 00:00:00 UTC 2020

Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection.

Frontiers in immunology

Casanova V,Sousa FH,Shakamuri P,Svoboda P,Buch C,D'Acremont M,Christophorou MA,Pohl J,Stevens C,Barlow PG

Wed Mar 03 00:00:00 UTC 2021

Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection.

Frontiers in immunology

Casanova V,Sousa FH,Shakamuri P,Svoboda P,Buch C,D'Acremont M,Christophorou MA,Pohl J,Stevens C,Barlow PG

Wed Mar 03 00:00:00 UTC 2021

Neutrophil extracellular traps mediate articular cartilage damage and enhance cartilage component immunogenicity in rheumatoid arthritis.

JCI insight

Carmona-Rivera C,Carlucci PM,Goel RR,James E,Brooks SR,Rims C,Hoffmann V,Fox DA,Buckner JH,Kaplan MJ

Thu Jul 09 00:00:00 UTC 2020

Effect of Porphyromonas gingivalis infection on gut dysbiosis and resultant arthritis exacerbation in mouse model.

Arthritis research & therapy

Hamamoto Y,Ouhara K,Munenaga S,Shoji M,Ozawa T,Hisatsune J,Kado I,Kajiya M,Matsuda S,Kawai T,Mizuno N,Fujita T,Hirata S,Tanimoto K,Nakayama K,Kishi H,Sugiyama E,Kurihara H

Mon Oct 19 00:00:00 UTC 2020

Peptidylarginine deiminase 4 overexpression resensitizes MCF-7/ADR breast cancer cells to adriamycin via GSK3β/p53 activation.

Cancer management and research

Zhou Q,Song C,Liu X,Qin H,Miao L,Zhang X

Thu Mar 31 00:00:00 UTC 2022

Peptidylarginine deiminase 4 overexpression resensitizes MCF-7/ADR breast cancer cells to adriamycin via GSK3β/p53 activation.

Cancer management and research

Zhou Q,Song C,Liu X,Qin H,Miao L,Zhang X

Thu Mar 31 00:00:00 UTC 2022

Inhibiting PAD2 enhances the anti-tumor effect of docetaxel in tamoxifen-resistant breast cancer cells.

Journal of experimental & clinical cancer research : CR

Li F,Miao L,Xue T,Qin H,Mondal S,Thompson PR,Coonrod SA,Liu X,Zhang X

Thu Oct 10 00:00:00 UTC 2019

Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa.

Science translational medicine

Byrd AS,Carmona-Rivera C,O'Neil LJ,Carlucci PM,Cisar C,Rosenberg AZ,Kerns ML,Caffrey JA,Milner SM,Sacks JM,Aliu O,Broderick KP,Reichner JS,Miller LS,Kang S,Robinson WH,Okoye GA,Kaplan MJ

Wed Sep 04 00:00:00 UTC 2019

Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa.

Science translational medicine

Byrd AS,Carmona-Rivera C,O'Neil LJ,Carlucci PM,Cisar C,Rosenberg AZ,Kerns ML,Caffrey JA,Milner SM,Sacks JM,Aliu O,Broderick KP,Reichner JS,Miller LS,Kang S,Robinson WH,Okoye GA,Kaplan MJ

Wed Sep 04 00:00:00 UTC 2019

Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription.

Molecular cell

Sharma P,Lioutas A,Fernandez-Fuentes N,Quilez J,Carbonell-Caballero J,Wright RHG,Di Vona C,Le Dily F,Schüller R,Eick D,Oliva B,Beato M

Thu Jan 03 00:00:00 UTC 2019

A Novel Citrullinated Modification of Histone 3 and Its Regulatory Mechanisms Related to IPO-38 Antibody-Labeled Protein.

Frontiers in oncology

Song S,Xiang Z,Li J,Ji J,Yan R,Zhu Z,Yu Y

Wed Sep 30 00:00:00 UTC 2020

A Novel Citrullinated Modification of Histone 3 and Its Regulatory Mechanisms Related to IPO-38 Antibody-Labeled Protein.

Frontiers in oncology

Song S,Xiang Z,Li J,Ji J,Yan R,Zhu Z,Yu Y

Wed Sep 30 00:00:00 UTC 2020

PAD2-Mediated Citrullination Contributes to Efficient Oligodendrocyte Differentiation and Myelination.

Cell reports

Falcão AM,Meijer M,Scaglione A,Rinwa P,Agirre E,Liang J,Larsen SC,Heskol A,Frawley R,Klingener M,Varas-Godoy M,Raposo AASF,Ernfors P,Castro DS,Nielsen ML,Casaccia P,Castelo-Branco G

Tue Apr 23 00:00:00 UTC 2019

PAD2-Mediated Citrullination Contributes to Efficient Oligodendrocyte Differentiation and Myelination.

Cell reports

Falcão AM,Meijer M,Scaglione A,Rinwa P,Agirre E,Liang J,Larsen SC,Heskol A,Frawley R,Klingener M,Varas-Godoy M,Raposo AASF,Ernfors P,Castro DS,Nielsen ML,Casaccia P,Castelo-Branco G

Tue Apr 23 00:00:00 UTC 2019

Modifying Enzymes Are Elicited by ER Stress, Generating Epitopes That Are Selectively Recognized by CD4⁺ T Cells in Patients With Type 1 Diabetes.

Diabetes

Marre ML,McGinty JW,Chow IT,DeNicola ME,Beck NW,Kent SC,Powers AC,Bottino R,Harlan DM,Greenbaum CJ,Kwok WW,Piganelli JD,James EA

Sun Jul 01 00:00:00 UTC 2018

Modifying Enzymes Are Elicited by ER Stress, Generating Epitopes That Are Selectively Recognized by CD4⁺ T Cells in Patients With Type 1 Diabetes.

Diabetes

Marre ML,McGinty JW,Chow IT,DeNicola ME,Beck NW,Kent SC,Powers AC,Bottino R,Harlan DM,Greenbaum CJ,Kwok WW,Piganelli JD,James EA

Sun Jul 01 00:00:00 UTC 2018

The Development of Benzimidazole-Based Clickable Probes for the Efficient Labeling of Cellular Protein Arginine Deiminases (PADs).

ACS chemical biology

Nemmara VV,Subramanian V,Muth A,Mondal S,Salinger AJ,Maurais AJ,Tilvawala R,Weerapana E,Thompson PR

Fri Mar 16 00:00:00 UTC 2018

The Development of Benzimidazole-Based Clickable Probes for the Efficient Labeling of Cellular Protein Arginine Deiminases (PADs).

ACS chemical biology

Nemmara VV,Subramanian V,Muth A,Mondal S,Salinger AJ,Maurais AJ,Tilvawala R,Weerapana E,Thompson PR

Fri Mar 16 00:00:00 UTC 2018

Activity-dependent Synaptic Recruitment of Neuroligin 1 in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Neuroscience

Zhao JY,Duan XL,Yang L,Liu JP,He YT,Guo Z,Hu XD,Suo ZW

Sat Sep 15 00:00:00 UTC 2018

Accumulation of citrullinated glial fibrillary acidic protein in a mouse model of bile duct ligation-induced hepatic fibrosis.

PloS one

Kim SE,Park JW,Kim MJ,Jang B,Jeon YC,Kim HJ,Ishigami A,Kim HS,Suk KT,Kim DJ,Park CK,Choi EK,Jang MK

Thu Jan 31 00:00:00 UTC 2019

Accumulation of citrullinated glial fibrillary acidic protein in a mouse model of bile duct ligation-induced hepatic fibrosis.

PloS one

Kim SE,Park JW,Kim MJ,Jang B,Jeon YC,Kim HJ,Ishigami A,Kim HS,Suk KT,Kim DJ,Park CK,Choi EK,Jang MK

Thu Jan 31 00:00:00 UTC 2019

Peptidylarginine deiminases 2 and 4 modulate innate and adaptive immune responses in TLR-7-dependent lupus.

JCI insight

Liu Y,Lightfoot YL,Seto N,Carmona-Rivera C,Moore E,Goel R,O'Neil L,Mistry P,Hoffmann V,Mondal S,Premnath PN,Gribbons K,Dell'Orso S,Jiang K,Thompson PR,Sun HW,Coonrod SA,Kaplan MJ

Thu Dec 06 00:00:00 UTC 2018

Peptidylarginine deiminases 2 and 4 modulate innate and adaptive immune responses in TLR-7-dependent lupus.

JCI insight

Liu Y,Lightfoot YL,Seto N,Carmona-Rivera C,Moore E,Goel R,O'Neil L,Mistry P,Hoffmann V,Mondal S,Premnath PN,Gribbons K,Dell'Orso S,Jiang K,Thompson PR,Sun HW,Coonrod SA,Kaplan MJ

Thu Dec 06 00:00:00 UTC 2018

Peptidylarginine deiminases 2 and 4 modulate innate and adaptive immune responses in TLR-7-dependent lupus.

JCI insight

Liu Y,Lightfoot YL,Seto N,Carmona-Rivera C,Moore E,Goel R,O'Neil L,Mistry P,Hoffmann V,Mondal S,Premnath PN,Gribbons K,Dell'Orso S,Jiang K,Thompson PR,Sun HW,Coonrod SA,Kaplan MJ

Thu Dec 06 00:00:00 UTC 2018

Peptidylarginine deiminases 2 and 4 modulate innate and adaptive immune responses in TLR-7-dependent lupus.

JCI insight

Liu Y,Lightfoot YL,Seto N,Carmona-Rivera C,Moore E,Goel R,O'Neil L,Mistry P,Hoffmann V,Mondal S,Premnath PN,Gribbons K,Dell'Orso S,Jiang K,Thompson PR,Sun HW,Coonrod SA,Kaplan MJ

Thu Dec 06 00:00:00 UTC 2018

BB-Cl-Amidine as a novel therapeutic for canine and feline mammary cancer via activation of the endoplasmic reticulum stress pathway.

BMC cancer

Ledet MM,Anderson R,Harman R,Muth A,Thompson PR,Coonrod SA,Van de Walle GR

Thu Apr 12 00:00:00 UTC 2018

BB-Cl-Amidine as a novel therapeutic for canine and feline mammary cancer via activation of the endoplasmic reticulum stress pathway.

BMC cancer

Ledet MM,Anderson R,Harman R,Muth A,Thompson PR,Coonrod SA,Van de Walle GR

Thu Apr 12 00:00:00 UTC 2018

BB-Cl-Amidine as a novel therapeutic for canine and feline mammary cancer via activation of the endoplasmic reticulum stress pathway.

BMC cancer

Ledet MM,Anderson R,Harman R,Muth A,Thompson PR,Coonrod SA,Van de Walle GR

Thu Apr 12 00:00:00 UTC 2018

BB-Cl-Amidine as a novel therapeutic for canine and feline mammary cancer via activation of the endoplasmic reticulum stress pathway.

BMC cancer

Ledet MM,Anderson R,Harman R,Muth A,Thompson PR,Coonrod SA,Van de Walle GR

Thu Apr 12 00:00:00 UTC 2018

Histone Citrullination Represses MicroRNA Expression, Resulting in Increased Oncogene mRNAs in Somatolactotrope Cells.

Molecular and cellular biology

DeVore SB,Young CH,Li G,Sundararajan A,Ramaraj T,Mudge J,Schilkey F,Muth A,Thompson PR,Cherrington BD

Mon Oct 01 00:00:00 UTC 2018

PADI2-Mediated Citrullination Promotes Prostate Cancer Progression.

Cancer research