Proteintech

PADI4 Polyclonal Antibody

PADI4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

PADI4 Antibody (17373-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed A549 cells using 17373-1-AP (PADI4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PADI4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PADI4 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17373-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 11 publications 11 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PADI4 Fusion Protein Ag11068 (317-663 aa encoded by BC025718)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EDFLKSVTT LAMKAKCKLT ICPEEENMDD QWMQDEMEIG YIQAPHKTLP VVFDSPRNRG LKEFPIKRVM GPDFGYVTRG PQTGGISGLD SFGNLEVSPP VTVRGKEYPL GRILFGDSCY PSNDSRQMHQ ALQDFLSAQQ VQAPVKLYSD WLSVGHVDEF LSFVPAPDRK GFRLLLASPR SCYKLFQEQQ NEGHGEALLF EGIKKKKQQK IKNILSNKTL REHNSFVERC IDWNRELLKR ELGLAESDII DIPQLFKLKE FSKAEAFFPN MVNMLVLGKH LGIPKPFGPV INGRCCLEEK VCSLLEPLGL QCTFINDFFT YHIRHGEVHC GTNVRRKPFS FKWWNMVP (317-663 aa encoded by BC025718)

Target Information

Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The role of protein arginine deiminase 4-dependent neutrophil extracellular traps formation in ulcerative colitis.

Frontiers in immunology

Wang P,Liu D,Zhou Z,Liu F,Shen Y,You Q,Lu S,Wu J

Mon May 08 00:00:00 UTC 2023

An African-Specific Variant of TP53 Reveals PADI4 as a Regulator of p53-Mediated Tumor Suppression.

Cancer discovery

Indeglia A,Leung JC,Miller SA,Leu JI,Dougherty JF,Clarke NL,Kirven NA,Shao C,Ke L,Lovell S,Barnoud T,Lu DY,Lin C,Kannan T,Battaile KP,Yang THL,Batista Oliva I,Claiborne DT,Vogel P,Liu L,Liu Q,Nefedova Y,Cassel J,Auslander N,Kossenkov AV,Karanicolas J,Murphy ME

Fri Jul 07 00:00:00 UTC 2023

An African-Specific Variant of TP53 Reveals PADI4 as a Regulator of p53-Mediated Tumor Suppression.

Cancer discovery

Fri Jul 07 00:00:00 UTC 2023

Sinomenine ameliorates adjuvant-induced arthritis by inhibiting the autophagy/NETosis/inflammation axis.

Scientific reports

Jiang H,Lu Q,Xu J,Huo G,Cai Y,Geng S,Xu H,Zhang J,Li H,Yuan K,Huang G

Thu Mar 09 00:00:00 UTC 2023

Sinomenine ameliorates adjuvant-induced arthritis by inhibiting the autophagy/NETosis/inflammation axis.

Scientific reports

Jiang H,Lu Q,Xu J,Huo G,Cai Y,Geng S,Xu H,Zhang J,Li H,Yuan K,Huang G

Thu Mar 09 00:00:00 UTC 2023

Forsythiaside B ameliorates coagulopathies in a rat model of sepsis through inhibition of the formation of PAD4-dependent neutrophil extracellular traps.

Frontiers in pharmacology

He W,Xi Q,Cui H,Zhang P,Huang R,Wang T,Wang D

Tue Nov 22 00:00:00 UTC 2022

Tetrandrine Ameliorates Rheumatoid Arthritis in Mice by Alleviating Neutrophil Activities.

Evidence-based complementary and alternative medicine : eCAM

Lu Q,Jiang H,Zhu Q,Xu J,Cai Y,Huo G,Yuan K,Huang G,Xu A

Sat Aug 24 00:00:00 UTC 2024

Inhibition of PAD4-mediated NET formation by cl-amidine prevents diabetes development in nonobese diabetic mice.

European journal of pharmacology

Shen Y,You Q,Wu Y,Wu J

Sat Feb 05 00:00:00 UTC 2022

Extracellular trap can be trained as a memory response.

Virulence

Gao Y,Zhang JG,Liu ZZ,Ma K,Lin XQ,Zhang JB,Chen W,Yang YJ

Thu Dec 01 00:00:00 UTC 2022

Neutrophil Extracellular Traps may be a Potential Target for Treating Early Brain Injury in Subarachnoid Hemorrhage.

Translational stroke research

Zeng H,Fu X,Cai J,Sun C,Yu M,Peng Y,Zhuang J,Chen J,Chen H,Yu Q,Xu C,Zhou H,Cao Y,Hu L,Li J,Cao S,Gu C,Yan F,Chen G

Tue Feb 01 00:00:00 UTC 2022

Neutrophil Extracellular Traps may be a Potential Target for Treating Early Brain Injury in Subarachnoid Hemorrhage.

Translational stroke research

Zeng H,Fu X,Cai J,Sun C,Yu M,Peng Y,Zhuang J,Chen J,Chen H,Yu Q,Xu C,Zhou H,Cao Y,Hu L,Li J,Cao S,Gu C,Yan F,Chen G

Tue Feb 01 00:00:00 UTC 2022

Neutrophil Extracellular Traps Caused by Gut Leakage Trigger the Autoimmune Response in Nonobese Diabetic Mice.

Frontiers in immunology

You Q,Shen Y,Wu Y,Li Y,Liu C,Huang F,Gu HF,Wu J

Wed Feb 23 00:00:00 UTC 2022

Investigation of H₂S Donor Treatment on Neutrophil Extracellular Traps in Experimental Colitis.

International journal of molecular sciences

Török S,Almási N,Valkusz Z,Pósa A,Varga C,Kupai K

Thu Nov 25 00:00:00 UTC 2021

Citrullinated inter-alpha-trypsin inhibitor heavy chain 4 in arthritic joints and its potential effect in the neutrophil migration.

Clinical and experimental immunology

Osada A,Matsumoto I,Mikami N,Ohyama A,Kurata I,Kondo Y,Tsuboi H,Ishigami A,Sano Y,Arai T,Ise N,Sumida T

Mon Mar 01 00:00:00 UTC 2021

Citrullinated inter-alpha-trypsin inhibitor heavy chain 4 in arthritic joints and its potential effect in the neutrophil migration.

Clinical and experimental immunology

Osada A,Matsumoto I,Mikami N,Ohyama A,Kurata I,Kondo Y,Tsuboi H,Ishigami A,Sano Y,Arai T,Ise N,Sumida T

Mon Mar 01 00:00:00 UTC 2021

TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage.

Frontiers in immunology

Wu X,Zeng H,Xu C,Chen H,Fan L,Zhou H,Yu Q,Fu X,Peng Y,Yan F,Yu X,Chen G

Fri Feb 18 00:00:00 UTC 2022

Innate and Autoimmunity in the Pathogenesis of Inherited Retinal Dystrophy.

Cells

Hollingsworth TJ,Gross AK

Thu Mar 05 00:00:00 UTC 2020

Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages.

Arthritis research & therapy

Trimova G,Yamagata K,Iwata S,Hirata S,Zhang T,Uemura F,Satoh M,Biln N,Nakayamada S,Maksymowych WP,Tanaka Y

Wed Feb 12 00:00:00 UTC 2020

Increased Protein Citrullination as a Trigger for Resident Immune System Activation, Intraretinal Inflammation, and Promotion of Anti-retinal Autoimmunity: Intersecting Paths in Retinal Degenerations of Potential Therapeutic Relevance.

Advances in experimental medicine and biology

Iannaccone A,Radic MZ

Wed Feb 05 00:00:00 UTC 2020

Thioredoxin Modulates Protein Arginine Deiminase 4 (PAD4)-Catalyzed Citrullination.

Frontiers in immunology

Nagar M,Tilvawala R,Thompson PR

Thu Sep 10 00:00:00 UTC 2020

Thioredoxin Modulates Protein Arginine Deiminase 4 (PAD4)-Catalyzed Citrullination.

Frontiers in immunology

Nagar M,Tilvawala R,Thompson PR

Thu Sep 10 00:00:00 UTC 2020

Cold-inducible RNA-binding Protein Induces Neutrophil Extracellular Traps in the Lungs during Sepsis.

Scientific reports

Ode Y,Aziz M,Jin H,Arif A,Nicastro JG,Wang P

Thu Apr 18 00:00:00 UTC 2019

IL-6-PAD4 axis in the earliest phase of arthritis in knock-in gp130F759 mice, a model for rheumatoid arthritis.

RMD open

Yahagi A,Saika T,Hirano H,Takai-Imamura M,Tsuji F,Aono H,Iseki M,Morita Y,Igarashi H,Saeki Y,Ishihara K

Tue Apr 28 00:00:00 UTC 2020

Murine Retinal Citrullination Declines With Age and is Mainly Dependent on Peptidyl Arginine Deiminase 4 (PAD4).

Investigative ophthalmology & visual science

Hollingsworth TJ,Radic MZ,Beranova-Giorgianni S,Giorgianni F,Wang Y,Iannaccone A

Wed Aug 01 00:00:00 UTC 2018

Murine Retinal Citrullination Declines With Age and is Mainly Dependent on Peptidyl Arginine Deiminase 4 (PAD4).

Investigative ophthalmology & visual science

Hollingsworth TJ,Radic MZ,Beranova-Giorgianni S,Giorgianni F,Wang Y,Iannaccone A

Wed Aug 01 00:00:00 UTC 2018

Activity-dependent Synaptic Recruitment of Neuroligin 1 in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Neuroscience

Zhao JY,Duan XL,Yang L,Liu JP,He YT,Guo Z,Hu XD,Suo ZW

Sat Sep 15 00:00:00 UTC 2018

Activity-dependent Synaptic Recruitment of Neuroligin 1 in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Neuroscience

Zhao JY,Duan XL,Yang L,Liu JP,He YT,Guo Z,Hu XD,Suo ZW

Sat Sep 15 00:00:00 UTC 2018

BB-Cl-Amidine as a novel therapeutic for canine and feline mammary cancer via activation of the endoplasmic reticulum stress pathway.

BMC cancer

Ledet MM,Anderson R,Harman R,Muth A,Thompson PR,Coonrod SA,Van de Walle GR

Thu Apr 12 00:00:00 UTC 2018

BB-Cl-Amidine as a novel therapeutic for canine and feline mammary cancer via activation of the endoplasmic reticulum stress pathway.

BMC cancer

Ledet MM,Anderson R,Harman R,Muth A,Thompson PR,Coonrod SA,Van de Walle GR

Thu Apr 12 00:00:00 UTC 2018

BB-Cl-Amidine as a novel therapeutic for canine and feline mammary cancer via activation of the endoplasmic reticulum stress pathway.

BMC cancer

Ledet MM,Anderson R,Harman R,Muth A,Thompson PR,Coonrod SA,Van de Walle GR

Thu Apr 12 00:00:00 UTC 2018

BB-Cl-Amidine as a novel therapeutic for canine and feline mammary cancer via activation of the endoplasmic reticulum stress pathway.

BMC cancer

Ledet MM,Anderson R,Harman R,Muth A,Thompson PR,Coonrod SA,Van de Walle GR

Thu Apr 12 00:00:00 UTC 2018

Neutrophil extracellular traps are critical for pregnancy loss in sphingosine kinase-deficient mice on 129Sv/C57BL/6 background.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Mizugishi K,Yamashita K

Fri Dec 01 00:00:00 UTC 2017

Neutrophil extracellular traps are critical for pregnancy loss in sphingosine kinase-deficient mice on 129Sv/C57BL/6 background.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Mizugishi K,Yamashita K

Fri Dec 01 00:00:00 UTC 2017

Decreased severity of experimental autoimmune arthritis in peptidylarginine deiminase type 4 knockout mice.

BMC musculoskeletal disorders

Suzuki A,Kochi Y,Shoda H,Seri Y,Fujio K,Sawada T,Yamada R,Yamamoto K

Thu May 05 00:00:00 UTC 2016

Decreased severity of experimental autoimmune arthritis in peptidylarginine deiminase type 4 knockout mice.

BMC musculoskeletal disorders

Suzuki A,Kochi Y,Shoda H,Seri Y,Fujio K,Sawada T,Yamada R,Yamamoto K

Thu May 05 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: HL-60 PAD; PAD type IV; PAD-R4; PADI-H protein; peptidyl arginine deiminase, type 4; peptidyl arginine deiminase, type IV; peptidyl arginine deiminase, type V; Peptidylarginine deiminase IV; Peptidylarginine deiminase type alpha; Protein-arginine deiminase type IV; Protein-arginine deiminase type-4

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Gene Aliases: PAD; PAD4; PADI4; PADI5; PDI4; PDI5

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UniProt ID: (Human) Q9UM07, (Rat) O88807, (Mouse) Q9Z183

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Entrez Gene ID: (Human) 23569, (Rat) 29512, (Mouse) 18602

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Function(s)

protein-arginine deiminase activity calcium ion binding protein binding arginine deiminase activity hydrolase activity metal ion binding

Process(es)

chromatin organization nucleosome assembly chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated cellular protein modification process chromatin modification protein citrullination stem cell population maintenance histone H3-R26 citrullination histone citrullination innate immune response immune system process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PADI4 Polyclonal Antibody

PADI4 Antibody (17373-1-AP) in ICC/IF

Product Details

17373-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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