Proteintech

PAF1 Polyclonal Antibody

PAF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PAF1 Antibody (15441-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using (Product # 15441-1-AP) (PAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PAF1 Antibody in Immunoprecipitation (IP)

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Product Details

15441-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PAF1 Fusion Protein Ag7212 (166-531 aa encoded by BC000017)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The MW of this protein is 80 kDa, and this antibody specifically recognizes the 80 kDa protein.

Immunogen sequence: EEIYKDRDS QITAIEKTFE DAQKSISQHY SKPRVTPVEV MPVFPDFKMW INPCAQVIFD SDPAPKDTSG AAALEMMSQA MIRGMMDEEG NQFVAYFLPV EETLKKRKRD QEEEMDYAPD DVYDYKIARE YNWNVKNKAS KGYEENYFFI FREGDGVYYN ELETRVRLSK RRAKAGVQSG TNALLVVKHR DMNEKELEAQ EARKAQLENH EPEEEEEEEM ETEEKEAGGS DEEQEKGSSS EKEGSEDEHS GSESEREEGD RDEASDKSGS GEDESSEDEA RAARDKEEIF GSDADSEDDA DSDDEDRGQA QGGSDNDSDS GSNGGGQRSR SHSRSASPFP SGSEHSAQED GSEAAASDSS EADSDSD (166-531 aa encoded by BC000017 )

Target Information

This gene encodes a subunit of the polymerase associated factor (PAF1) complex. The PAF1 complex interacts with RNA polymerase II and plays a role in transcription elongation as well as histone modifications including ubiquitylation and methylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation.

Science advances

Qiu M,Yin Z,Wang H,Lei L,Li C,Cui Y,Dai R,Yang P,Xiang Y,Li Q,Lv J,Hu Z,Chen M,Zhou HB,Fang P,Xiao R,Liang K

Fri May 19 00:00:00 UTC 2023

Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening.

ACS pharmacology & translational science

Wang L,Li C,Chen P,Liu C,Cui Z,Deng S,Yang H

Fri Jul 08 00:00:00 UTC 2022

Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.

The Journal of cell biology

Chen C,Yamanaka Y,Ueda K,Li P,Miyagi T,Harada Y,Tezuka S,Narumi S,Sugimoto M,Kuroda M,Hayamizu Y,Kanekura K

Mon Nov 01 00:00:00 UTC 2021

The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.

Autophagy

Zheng L,Shu WJ,Li YM,Mari M,Yan C,Wang D,Yin ZH,Jiang W,Zhou Y,Okamoto K,Reggiori F,Klionsky DJ,Song Z,Du HN

Sat Aug 01 00:00:00 UTC 2020

Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcription.

Radiation oncology (London, England)

Zheng JJ,He Y,Liu Y,Li FS,Cui Z,Du XM,Wang CP,Wu YM

Fri May 29 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: hPAF1; Paf1, RNA polymerase II associated factor, homolog; Pancreatic differentiation protein 2; RNA polymerase II-associated factor 1 homolog

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Gene Aliases: F23149_1; PAF1; PD2

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UniProt ID: (Human) Q8N7H5

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Entrez Gene ID: (Human) 54623

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Function(s)

RNA polymerase II core binding chromatin binding protein binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter endodermal cell fate commitment transcription elongation from RNA polymerase II promoter mRNA polyadenylation histone monoubiquitination Wnt signaling pathway nucleosome positioning stem cell population maintenance positive regulation of histone methylation positive regulation of mRNA 3'-end processing positive regulation of transcription elongation from RNA polymerase II promoter histone H2B ubiquitination protein localization to nucleus negative regulation of myeloid cell differentiation positive regulation of transcription from RNA polymerase II promoter cellular response to lipopolysaccharide

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PAF1 Polyclonal Antibody

PAF1 Antibody (15441-1-AP) in IHC (P)

Product Details

15441-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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