Proteintech

PAICS Polyclonal Antibody

PAICS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PAICS Antibody (12967-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human liver tissue slide using (Product # 12967-1-AP) PAICS antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

12967-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PAICS Fusion Protein Ag3621 (76-425 aa encoded by BC010273)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: AFTRKCGET AFIAPQCEMI PIEWVCRRIA TGSFLKRNPG VKEGYKFYPP KVELFFKDDA NNDPQWSEEQ LIAAKFCFAG LLIGQTEVDI MSHATQAIFE ILEKSWLPQN CTLVDMKIEF GVDVTTKEIV LADVIDNDSW RLWPSGDRSQ QKDKQSYRDL KEVTPEGLQM VKKNFEWVAE RVELLLKSES QCRVVVLMGS TSDLGHCEKI KKACGNFGIP CELRVTSAHK GPDETLRIKA EYEGDGIPTV FVAVAGRSNG LGPVMSGNTA YPVISCPPLT PDWGVQDVWS SLRLPSGLGC STVLSPEGSA QFAAQIFGLS NHLVWSKLRA SILNTWISLK QADKKIRECN L (76-425 aa encoded by BC010273)

Target Information

This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Therapeutic targeting of metabolic vulnerabilities in cancers with MLL3/4-COMPASS epigenetic regulator mutations.

The Journal of clinical investigation

Zhao Z,Cao K,Watanabe J,Philips CN,Zeidner JM,Ishi Y,Wang Q,Gold SR,Junkins K,Bartom ET,Yue F,Chandel NS,Hashizume R,Ben-Sahra I,Shilatifard A

Mon Jul 03 00:00:00 UTC 2023

ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.

Molecular cell

Ali ES,Sahu U,Villa E,O'Hara BP,Gao P,Beaudet C,Wood AW,Asara JM,Ben-Sahra I

Thu Jun 18 00:00:00 UTC 2020

Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex.

iScience

Yamada S,Sato A,Sakakibara SI

Fri May 22 00:00:00 UTC 2020

Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex.

iScience

Yamada S,Sato A,Sakakibara SI

Fri May 22 00:00:00 UTC 2020

Knockdown of PAICS inhibits malignant proliferation of human breast cancer cell lines.

Biological research

Meng M,Chen Y,Jia J,Li L,Yang S

Fri Aug 10 00:00:00 UTC 2018

Knockdown of PAICS inhibits malignant proliferation of human breast cancer cell lines.

Biological research

Meng M,Chen Y,Jia J,Li L,Yang S

Fri Aug 10 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: AIR carboxylase; Bifunctional phosphoribosylaminoimidazole carboxylase/phosphoribosylaminoimidazole succinocarboxamide synthetase; multifunctional protein ADE2; multifunctional protein ADE2H1; PAICS; phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase; SAICAR synthetase

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Gene Aliases: 2610511I09Rik; ADE2; ADE2H1; AIRC; PAICS; PAIS

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UniProt ID: (Human) P22234, (Mouse) Q9DCL9

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Entrez Gene ID: (Human) 10606, (Mouse) 67054

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Function(s)

phosphoribosylaminoimidazole carboxylase activity phosphoribosylaminoimidazolesuccinocarboxamide synthase activity protein binding ATP binding identical protein binding cadherin binding involved in cell-cell adhesion nucleotide binding catalytic activity lyase activity carboxy-lyase activity ligase activity

Process(es)

'de novo' IMP biosynthetic process purine nucleobase biosynthetic process purine ribonucleoside monophosphate biosynthetic process adenine biosynthetic process cell-cell adhesion purine nucleotide biosynthetic process metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PAICS Polyclonal Antibody

PAICS Antibody (12967-1-AP) in IHC (P)

Product Details

12967-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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