Proteintech

PAIP1 Polyclonal Antibody

View all (11) PAIP1 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (6)

PAIP1 Antibody in Immunocytochemistry (ICC/IF)

PAIP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

PAIP1 Antibody (10675-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using PAIP1 antibody 10675-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

PAIP1 Antibody in Immunocytochemistry (ICC/IF)

PAIP1 Antibody in Western Blot (WB)

PAIP1 Antibody in Western Blot (WB)

PAIP1 Antibody in Western Blot (WB)

PAIP1 Antibody in Western Blot (WB)

PAIP1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

PAIP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using PAIP1 antibody 10675-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

PAIP1 Antibody in Western Blot (WB) — BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PAIP1 Antibody in Western Blot (WB) — COS-7 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PAIP1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PAIP1 Antibody in Western Blot (WB) — BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

PAIP1 Antibody in Immunoprecipitation (IP) — IP result of anti-PAIP1 (IP:10675-1-AP, 4ug; Detection:10675-1-AP 1:300) with HeLa cells lysate 3000 ug.

Product Details

10675-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

Assay-dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

-

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PAIP1 Fusion Protein Ag1070 (180-479 aa encoded by BC005295)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EQFAETLNG CVTTDDALQE LVELIYQQAT SIPNFSYMGA RLCNYLSHHL TISPQSGNFR QLLLQRCRTE YEVKDQAAKG DEVTRKRFHA FVLFLGELYL NLEIKGTNGQ VTRADILQVG LRELLNALFS NPMDDNLICA VKLLKLTGSV LEDAWKEKGK MDMEEIIQRI ENVVLDANCS RDVKQMLLKL VELRSSNWGR VHATSTYREA TPENDPNYFM NEPTFYTSDG VPFTAADPDY QEKYQELLER EDFFPDYEEN GTDLSGAGDP YLDDIDDEMD PEIEEAYEKF CLESERKRKQ (180-479 aa encoded by BC005295)

Target Information

The protein encoded by this gene interacts with poly(A)-binding protein and with the cap-binding complex eIF4A. It is involved in translational initiation and protein biosynthesis. Overexpression of this gene in COS7 cells stimulates translation. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2†.

Biology of reproduction

He Y,Lin Y,Zhu Y,Ping P,Wang G,Sun F

Fri Feb 01 00:00:00 UTC 2019

Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2†.

Biology of reproduction

He Y,Lin Y,Zhu Y,Ping P,Wang G,Sun F

Fri Feb 01 00:00:00 UTC 2019

Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2†.

Biology of reproduction

He Y,Lin Y,Zhu Y,Ping P,Wang G,Sun F

Fri Feb 01 00:00:00 UTC 2019

Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2†.

Biology of reproduction

He Y,Lin Y,Zhu Y,Ping P,Wang G,Sun F

Fri Feb 01 00:00:00 UTC 2019

Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2†.

Biology of reproduction

He Y,Lin Y,Zhu Y,Ping P,Wang G,Sun F

Fri Feb 01 00:00:00 UTC 2019

Paip1 predicts poor prognosis and promotes tumor progression through AKT/GSK-3β pathway in lung adenocarcinoma.

Human pathology

Wang Y,Piao J,Wang Q,Cui X,Meng Z,Jin T,Lin Z

Mon Apr 01 00:00:00 UTC 2019

Paip1 predicts poor prognosis and promotes tumor progression through AKT/GSK-3β pathway in lung adenocarcinoma.

Human pathology

Wang Y,Piao J,Wang Q,Cui X,Meng Z,Jin T,Lin Z

Mon Apr 01 00:00:00 UTC 2019

Paip1 predicts poor prognosis and promotes tumor progression through AKT/GSK-3β pathway in lung adenocarcinoma.

Human pathology

Wang Y,Piao J,Wang Q,Cui X,Meng Z,Jin T,Lin Z

Mon Apr 01 00:00:00 UTC 2019

Paip1 predicts poor prognosis and promotes tumor progression through AKT/GSK-3β pathway in lung adenocarcinoma.

Human pathology

Wang Y,Piao J,Wang Q,Cui X,Meng Z,Jin T,Lin Z

Mon Apr 01 00:00:00 UTC 2019

Paip1 Indicated Poor Prognosis in Cervical Cancer and Promoted Cervical Carcinogenesis.

Cancer research and treatment

Li N,Piao J,Wang X,Kim KY,Bae JY,Ren X,Lin Z

Tue Oct 01 00:00:00 UTC 2019

Paip1 Indicated Poor Prognosis in Cervical Cancer and Promoted Cervical Carcinogenesis.

Cancer research and treatment

Li N,Piao J,Wang X,Kim KY,Bae JY,Ren X,Lin Z

Tue Oct 01 00:00:00 UTC 2019

Role of Paip1 on angiogenesis and invasion in pancreatic cancer.

Experimental cell research

Guan H,Li N,Wang X,Shan X,Li Z,Lin Z

Fri Mar 15 00:00:00 UTC 2019

Role of Paip1 on angiogenesis and invasion in pancreatic cancer.

Experimental cell research

Guan H,Li N,Wang X,Shan X,Li Z,Lin Z

Fri Mar 15 00:00:00 UTC 2019

Biological insights into the expression of translation initiation factors from recombinant CHOK1SV cell lines and their relationship to enhanced productivity.

The Biochemical journal

Mead EJ,Masterton RJ,Feary M,Obrezanova O,Zhang L,Young R,Smales CM

Tue Dec 15 00:00:00 UTC 2015

Biological insights into the expression of translation initiation factors from recombinant CHOK1SV cell lines and their relationship to enhanced productivity.

The Biochemical journal

Mead EJ,Masterton RJ,Feary M,Obrezanova O,Zhang L,Young R,Smales CM

Tue Dec 15 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: PABC1-interacting protein 1; PABP-interacting protein 1; PAIP-1; poly(A)-binding protein-interacting protein 1; polyadenylate binding protein-interacting protein 1; Polyadenylate-binding protein-interacting protein 1

View more View less

Gene Aliases: PAIP1

View more View less

UniProt ID: (Human) Q9H074

View more View less

Entrez Gene ID: (Human) 10605

View more View less

RNA binding protein binding translation activator activity

nuclear-transcribed mRNA poly(A) tail shortening translational initiation positive regulation of translation mRNA stabilization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support