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Product Details
H00005063-M07
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
1H7
Immunogen
PAK3 (NP_002569, 1 a.a. approximately 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: MSDGLDNEEK PPAPPLRMNS NNRDSSALNH SSKPLPMAPE EKNKKARLRS IFPGGGDKTN KKKEKERPEI SLPSDFEHTI HVGFDAVTGE
Target Information
PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2.7.11.1; adriamycin resistance-associated; Beta-PAK; CDC42/RAC effector kinase PAK-B; CDKN1A; hPAK3; Oligophrenin-3; p21 (CDKN1A)-activated kinase 3; p21 protein (Cdc42/Rac)-activated kinase 3; p21-activated kinase 3; PAK beta; PAK-3; Serine/threonine-protein kinase PAK 3
Gene Aliases: ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3; PAK3beta; Pak65alpha; Pak65beta; Pakb; Stk4
UniProt ID: (Human) O75914, (Mouse) Q61036
Entrez Gene ID: (Human) 5063, (Mouse) 18481
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase activity
protein binding
ATP binding
Rho GTPase binding
SH3 domain binding
metal ion binding
nucleotide binding
catalytic activity
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Rac GTPase binding
GTPase binding
MAPK cascade
activation of MAPK activity
protein phosphorylation
Rho protein signal transduction
multicellular organism development
regulation of mitotic cell cycle
axonogenesis
metabolic process
regulation of neuron projection development
phosphorylation
dendrite development
cell migration
signal transduction by protein phosphorylation
actin cytoskeleton organization
regulation of actin filament polymerization
stress-activated protein kinase signaling cascade
activation of protein kinase activity
regulation of apoptotic process
regulation of MAPK cascade
positive regulation of neuron apoptotic process
dendritic spine development
positive regulation of dendritic spine morphogenesis
cellular response to organic cyclic compound
positive regulation of DNA biosynthetic process
mitotic cell cycle
stimulatory C-type lectin receptor signaling pathway
T cell costimulation
ephrin receptor signaling pathway
synapse organization
T cell receptor signaling pathway
dendritic spine morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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