Proteintech

PAK6 Polyclonal Antibody

PAK6 Antibody in Immunocytochemistry (ICC/IF)

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PAK6 Antibody (13539-1-AP) in ICC/IF

Immunofluorescent analysis of HEK-293 cells using 13539-1-AP (PAK6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PAK6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PAK6 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13539-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PAK6 Fusion Protein Ag4467 (330-681 aa encoded by BC035596)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody has weak cross-reaction with other PAK members.

Immunogen sequence: QKSLRTAPA TGQLPGRSSP AGSPRTWHAQ ISTSNLYLPQ DPTVAKGALA GEDTGVVTHE QFKAALRMVV DQGDPRLLLD SYVKIGEGST GIVCLAREKH SGRQVAVKMM DLRKQQRREL LFNEVVIMRD YQHFNVVEMY KSYLVGEELW VLMEFLQGGA LTDIVSQVRL NEEQIATVCE AVLQALAYLH AQGVIHRDIK SDSILLTLDG RVKLSDFGFC AQISKDVPKR KSLVGTPYWM APEVISRSLY ATEVDIWSLG IMVIEMVDGE PPYFSDSPVQ AMKRLRDSPP PKLKNSHKVS PVLRDFLERM LVRDPQERAT AQELLDHPFL LQTGLPECLV PLIQLYRKQT STC (330-681 aa encoded by BC035596)

Target Information

PAK6 is a serine/threonine kinase expressed in the testis and prostate. It associates with both the estrogen and androgen receptors. The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. Later experiments have shown it to be activated by MAP kinase kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PAK6 promotes cervical cancer progression through activation of the Wnt/β-catenin signaling pathway.

Oncology letters

Yang Q,Zhao Y,Chen Y,Chang Y,Huang A,Xu T,Li G,Wu G

Tue Sep 01 00:00:00 UTC 2020

PAK6 promotes cervical cancer progression through activation of the Wnt/β-catenin signaling pathway.

Oncology letters

Yang Q,Zhao Y,Chen Y,Chang Y,Huang A,Xu T,Li G,Wu G

Tue Sep 01 00:00:00 UTC 2020

Proteomics analysis of the amygdala in rats with CFA-induced pain aversion with electro-acupuncture stimulation.

Journal of pain research

Wu Y,Jiang Y,Shao X,He X,Shen Z,Shi Y,Wang C,Fang J

Tue Apr 12 00:00:00 UTC 2022

MicroRNA‑328 directly targets p21‑activated protein kinase 6 inhibiting prostate cancer proliferation and enhancing docetaxel sensitivity.

Molecular medicine reports

Liu C,Zhang L,Huang Y,Lu K,Tao T,Chen S,Zhang X,Guan H,Chen M,Xu B

Sun Nov 01 00:00:00 UTC 2015

MicroRNA‑328 directly targets p21‑activated protein kinase 6 inhibiting prostate cancer proliferation and enhancing docetaxel sensitivity.

Molecular medicine reports

Liu C,Zhang L,Huang Y,Lu K,Tao T,Chen S,Zhang X,Guan H,Chen M,Xu B

Sun Nov 01 00:00:00 UTC 2015

MicroRNA‑328 directly targets p21‑activated protein kinase 6 inhibiting prostate cancer proliferation and enhancing docetaxel sensitivity.

Molecular medicine reports

Liu C,Zhang L,Huang Y,Lu K,Tao T,Chen S,Zhang X,Guan H,Chen M,Xu B

Sun Nov 01 00:00:00 UTC 2015

MicroRNA‑328 directly targets p21‑activated protein kinase 6 inhibiting prostate cancer proliferation and enhancing docetaxel sensitivity.

Molecular medicine reports

Liu C,Zhang L,Huang Y,Lu K,Tao T,Chen S,Zhang X,Guan H,Chen M,Xu B

Sun Nov 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: p21 (CDKN1A)-activated kinase 6; p21 protein (Cdc42/Rac)-activated kinase 6; p21(CDKN1A)-activated kinase 6; p21-activated kinase 6; PAK-5; PAK-6; Serine/threonine-protein kinase PAK 6

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Gene Aliases: 4732456M09; PAK5; PAK6

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UniProt ID: (Human) Q9NQU5, (Mouse) Q3ULB5

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Entrez Gene ID: (Human) 56924, (Mouse) 214230, (Rat) 296078

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Function(s)

receptor signaling protein serine/threonine kinase activity protein binding ATP binding cadherin binding involved in cell-cell adhesion nucleotide binding protein kinase activity protein serine/threonine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Rac GTPase binding

Process(es)

regulation of transcription, DNA-templated protein phosphorylation Rho protein signal transduction regulation of mitotic cell cycle learning memory locomotory behavior phosphorylation cell migration signal transduction by protein phosphorylation actin cytoskeleton organization stress-activated protein kinase signaling cascade activation of protein kinase activity regulation of apoptotic process regulation of MAPK cascade mitotic cell cycle apoptotic process cytoskeleton organization cell-cell adhesion intracellular signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PAK6 Polyclonal Antibody

PAK6 Antibody (13539-1-AP) in ICC/IF

Product Details

13539-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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