Invitrogen

PAQR3 Polyclonal Antibody

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PAQR3 Antibody (PA5-24654) in WB

Western blot analysis of PAQR3 in WiDr cell line lysates. Samples were incubated with PAQR3 polyclonal antibody (Product # PA5-24654). Lysates: 35 µg/lane. PAQR3 (arrow).

Product Image Gallery

PAQR3 Antibody — Figure 2 PAQR3 modulates ATG14L-linked VPS34 lipid kinase activity A-C HeLa cells stably expressing GFP-DFCP1 were transfected with or without PAQR3-specific shRNA. GFP signals were observed in these cells before or after glucose starvation (GS, shown in A). The knockdown efficiency of PAQR3 is shown in (B), and the statistics of GFP-positive puncta per cell is illustrated in (C). Scale bar: 10 µm. D GFP-WIPI1 was transfected into WT or PAQR3-deficient MEFs. Twenty-four hours later, the cells were incubated under GS for 4 h, followed by fluorescence observation. Scale bar: 10 µm. E Different VPS34 complexes of WT or PAQR3 knockout HeLa cells were immunoprecipitated by ATG14L and UVRAG antibodies, respectively, in normal medium (NM) or GS. Then, PI(3)P level was determined by a quantitative ELISA. The PI(3)P level was normalized to the amount of ATG14L or UVRAG used in the assay. F HeLa cells infected with control or PAQR3-overexpressed lentivirus were treated with GS for 4 h. PI(3)P levels were determined as in Fig 2E.G, H PAQR3 knockout HeLa cells were transfected with plasmids of multiple PAQR3 deletion mutants. At 24 h after transfection, the cell lysates were used to immunoprecipitate VPS34 complex by ATG14L antibody, followed by immunoblotting. The activity of ATG14L-linked VPS34 activity was detected by a quantitative ELISA. The PI(3)P level was normalized to the amount of ATG14L. Data information: Values are presented as mean ± SD (n = 5; **P < 0.01; ***P < 0.001; ns, not significant). {KD}

Product Details

PA5-24654

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

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Immunoprecipitation (IP)

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 242-270 amino acids from the C-terminal region of human PAQR3

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2542154

Product Specific Information

This antibody is predicted to react with mouse based on sequence homology.

Target Information

PAQR3 is function as a spatial regulator of RAF1 kinase by sequestrating it to the Golgi.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PAQR3 modulates cholesterol homeostasis by anchoring Scap/SREBP complex to the Golgi apparatus.

Nature communications

Xu D,Wang Z,Zhang Y,Jiang W,Pan Y,Song BL,Chen Y

PA5-24654 was used in immunoprecipitation and western blot to report that PAQR3 regulates cholesterol homeostasis by anchoring Scap/SREBP to the Golgi

Thu Aug 27 00:00:00 UTC 2015

PAQR3 modulates cholesterol homeostasis by anchoring Scap/SREBP complex to the Golgi apparatus.

Nature communications

Xu D,Wang Z,Zhang Y,Jiang W,Pan Y,Song BL,Chen Y

PA5-24654 was used in immunoprecipitation and western blot to report that PAQR3 regulates cholesterol homeostasis by anchoring Scap/SREBP to the Golgi

Thu Aug 27 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Progestin and adipoQ receptor family member 3; Progestin and adipoQ receptor family member III; Raf kinase trapping to Golgi; RKTG

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Gene Aliases: PAQR3; RKTG

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UniProt ID: (Human) Q6TCH7

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Entrez Gene ID: (Human) 152559

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Function(s)

receptor activity

Process(es)

MAPK cascade negative regulation of protein phosphorylation negative regulation of neuron projection development negative regulation of peptidyl-serine phosphorylation protein localization to Golgi apparatus negative regulation of MAP kinase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PAQR3 Polyclonal Antibody

PAQR3 Antibody (PA5-24654) in WB

Product Details

PA5-24654

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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