Invitrogen

PAR2 Monoclonal Antibody (SAM11)

Advanced Verification

4 Published Figures

11 References

View all (17) PAR2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (4)
Advanced Verification (1)

PAR2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

PAR2 Antibody (35-2300) in ICC/IF

Cell communication and signaling : CCS 2018 - Fig. 5 Expression of PAR2 in HFL-1 cells. RTqPCR was used to confirm the expression of the serine protease receptor PAR2 on HFL-1. The relative gene expression was related to the housekeeping gene 18 s ( n = 2, 1 experiment). Human lung fibroblasts, HFL-1, showed a high gene expression for PAR2, while LAD2 cells did not show any PAR2 gene expression ( a ). Expression of PAR2 examined in monolayer cultures o... View More

PAR2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

PAR2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PAR2 Antibody — FIG. 4. The HT29/C1 cell receptor for BFT is a membrane protein other than E-cadherin. (A) Confocal microscopic examination of control subconfluent HT29/C1 cells stained with an E-cadherin antibody (C36) demonstrates diffuse membrane staining (panel 1). HT29/C1 cells treated with Alexa 488-labeled BFT (6 nM) for 1 hour at 37degC demonstrate the near absence of E-cadherin staining (panel 2) on cells binding Alexa 488-labeled BFT (panel 4). Panel 3 demonstrates low background fluorescence in untreated control cells in the channel detecting Alexa 488-labeled BFT. (B) PAR2 does not colocalize with Alexa 488-labeled-BFT cell binding. Panel A shows the distribution of PAR2 (red) in control HT29/C1 cells. Panel B shows no colocalization of PAR2 and Alexa 488-labeled-BFT (green) binding to HT29/C1 cells (10 nM, 1 h, 37degC). Panel C shows downregulation of PAR2 immunostaining by treatment of HT29/C1 cells with trypsin (10 nM, 15 min, 37degC). Panel D shows that Alexa 488-labeled- BFT staining remains intact when PAR2 is downregulated by trypsin treatment. {TM}

Product Details

35-2300

Applications

Tested Dilution

Publications

Western Blot (WB)

View 3 publications 3 publications

Immunohistochemistry (IHC)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

SAM11

Immunogen

A synthetic peptide corresponding to the N-terminal of the human PAR2 receptor.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533199

Target Information

Coagulation factor II (thrombin) receptor-like 1 (F2RL1) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL1 is also a member of the protease-activated receptor family. It is activated by trypsin, but not by thrombin. It is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. The F2RL1 gene contains two exons and is widely expressed in human tissues. The predicted protein sequence is 83% identical to the mouse receptor sequence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mast Cell/Proteinase Activated Receptor 2 (PAR2) Mediated Interactions in the Pathogenesis of Discogenic Back Pain.

Frontiers in cellular neuroscience

Richards J,Tang S,Gunsch G,Sul P,Wiet M,Flanigan DC,Khan SN,Moore S,Walter B,Purmessur D

35-2300 was used in Immunohistochemistry to examine the therapeutic potential of PAR2 antagonist (PAR2A) pointing toward structural restoration of the bovine IVD including down-regulation of VEGFA.

Thu Oct 01 00:00:00 UTC 2020

Mast Cell/Proteinase Activated Receptor 2 (PAR2) Mediated Interactions in the Pathogenesis of Discogenic Back Pain.

Frontiers in cellular neuroscience

Richards J,Tang S,Gunsch G,Sul P,Wiet M,Flanigan DC,Khan SN,Moore S,Walter B,Purmessur D

35-2300 was used in Immunohistochemistry to examine the therapeutic potential of PAR2 antagonist (PAR2A) pointing toward structural restoration of the bovine IVD including down-regulation of VEGFA.

Thu Oct 01 00:00:00 UTC 2020

Mast cells and mast cell tryptase enhance migration of human lung fibroblasts through protease-activated receptor 2.

Cell communication and signaling : CCS

Bagher M,Larsson-Callerfelt AK,Rosmark O,Hallgren O,Bjermer L,Westergren-Thorsson G

35-2300 was used in Immunocytochemistry-immunoflourescence to demonstrate roles for mast cells and the mast cell mediator tryptase in inducing lung fibroblast migration via PAR-2 activation.

Sat Sep 15 00:00:00 UTC 2018

Mast cells and mast cell tryptase enhance migration of human lung fibroblasts through protease-activated receptor 2.

Cell communication and signaling : CCS

Bagher M,Larsson-Callerfelt AK,Rosmark O,Hallgren O,Bjermer L,Westergren-Thorsson G

35-2300 was used in Immunocytochemistry-immunoflourescence to demonstrate roles for mast cells and the mast cell mediator tryptase in inducing lung fibroblast migration via PAR-2 activation.

Sat Sep 15 00:00:00 UTC 2018

Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5.

PloS one

Huang LY,Stuart C,Takeda K,D'Agnillo F,Golding B

35-2300 was used in immunocytochemistry assess the effect of polyinosinic-polycytidylic acid on the barrier function and tight junction integrity of primary human lung microvascular endothelial cells

Fri Aug 04 00:00:00 UTC 2017

Retinal neurons curb inflammation and enhance revascularization in ischemic retinopathies via proteinase-activated receptor-2.

The American journal of pathology

Sitaras N,Rivera JC,Noueihed B,Bien-Aimé M,Zaniolo K,Omri S,Hamel D,Zhu T,Hardy P,Sapieha P,Joyal JS,Chemtob S

35-2300 was used in immunohistochemistry and western blot to test if proteinase-activated receptor-2 modulates the deleterious effects of IL-1beta in ischemic retinopathies.

Sun Feb 01 00:00:00 UTC 2015

Retinal neurons curb inflammation and enhance revascularization in ischemic retinopathies via proteinase-activated receptor-2.

The American journal of pathology

Sitaras N,Rivera JC,Noueihed B,Bien-Aimé M,Zaniolo K,Omri S,Hamel D,Zhu T,Hardy P,Sapieha P,Joyal JS,Chemtob S

35-2300 was used in immunohistochemistry and western blot to test if proteinase-activated receptor-2 modulates the deleterious effects of IL-1beta in ischemic retinopathies.

Sun Feb 01 00:00:00 UTC 2015

Analysis of protease-activated receptor-1 and -2 in human scar formation.

The Journal of pathology

Materazzi S,Pellerito S,Di Serio C,Paglierani M,Naldini A,Ardinghi C,Carraro F,Geppetti P,Cirino G,Santucci M,Tarantini F,Massi D

35-2300 was used in immunohistochemistry (paraffin) to examine expression and biological activity of PAR-1 and PAR-2 in normal and pathological cutaneous scars.

Wed Aug 01 00:00:00 UTC 2007

Expression of protease-activated receptor-1 and -2 in orofacial granulomatosis.

Oral diseases

Ketabchi S,Massi D,Ficarra G,Rubino I,Franchi A,Paglierani M,Simoni A,Capodiferro S,Favia G,Maiorano E,Tarantini F,Cirino G,Santucci M

35-2300 was used in immunohistochemistry - paraffin section to determine the expression of PAR-1, PAR-2, MMP-2, MMP-9, COX-1, and COX-2 in patients with orofacial granulomatosis

Sun Jul 01 00:00:00 UTC 2007

The Bacteroides fragilis toxin binds to a specific intestinal epithelial cell receptor.

Infection and immunity

Wu S,Shin J,Zhang G,Cohen M,Franco A,Sears CL

35-2300 was used in immunocytochemistry to look for a cellular receptor for Bacteroides fragilis toxin on intestinal epithelial cells

Fri Sep 01 00:00:00 UTC 2006

Evidence that protease activated receptor 2 expression is enhanced in human coronary atherosclerotic lesions.

Journal of clinical pathology

Napoli C,de Nigris F,Wallace JL,Hollenberg MD,Tajana G,De Rosa G,Sica V,Cirino G

Sat May 01 00:00:00 UTC 2004

Constitutive protease-activated receptor-2-mediated migration of MDA MB-231 breast cancer cells requires both beta-arrestin-1 and -2.

The Journal of biological chemistry

Ge L,Shenoy SK,Lefkowitz RJ,DeFea K

Fri Dec 31 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: coagulation factor II (thrombin) receptor-like 1; Coagulation factor II receptor-like 1; G-protein coupled receptor 11; PAR-2; protease-activated receptor 2; Proteinase-activated receptor 2; Thrombin receptor-like 1

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Gene Aliases: F2RL1; GPR11; PAR2

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UniProt ID: (Human) P55085

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Entrez Gene ID: (Human) 2150

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Function(s)

G-protein alpha-subunit binding receptor activity G-protein coupled receptor activity receptor binding protein binding thrombin receptor activity G-protein beta-subunit binding

Process(es)

Performance Guarantee

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PAR2 Monoclonal Antibody (SAM11)

PAR2 Antibody (35-2300) in ICC/IF

Product Details

35-2300

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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