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PARC/H7-AP1 Antibody (A300-098A) in WB
Detection of human PARC by western blot and immunoprecipitation. Samples: A) Whole cell lysate (100 µg) from untreated U2OS cells or U2OS derived cells that stably express a short hairpin RNA (shPARC) against PARC. B) Whole cell lysate (200 µg for input; 1 mg for IP) from BJAB cells. Antibodies: Affinity purified rabbit anti-PARC antibody (Product # A300-098A) used at 0.04 µg/mL for WB (A & B) and at 2 µg/mg lysate for IP. PARC was also immunoprecipitated using A300-096A at 2 µg/mg lysate. Detection: Chemiluminescence with an exposure time... View More
Product Details
A300-098A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 2475 and the C-terminus (residue 2527) of human P53-associated parkin-like cytoplasmic protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The continued localization of p53 to the nucleus is essential for its function as a tumor suppressor. PARC, a large, Parkin-like ubiquitin ligase has recently been identified as a cytoplasmic anchor protein in p53-associated protein complexes. In the absence of stress, PARC inactivation results in nuclear localization of p53 and activation of p53-dependent apoptosis, while overexpression of this protein promoted cytoplasmic sequestration of p53. Surprisingly, PARC knockout mice were viable and exhibited no obvious phenotype, suggesting that other proteins, such as the highly related cullin family of E3 ubiquitin ligases, may perform similar functions in the absence of PARC. Additionally, it has been suggested that p53 binding to PARC may serve to control PARC function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CUL-9; Cullin-9; p53-associated parkin-like cytoplasmic protein; parkin-like cytoplasmic p53 binding protein; RP3-330M21.2; UbcH7-associated protein 1
Gene Aliases: CUL9; H7AP1; KIAA0708; PARC
UniProt ID: (Human) Q8IWT3
Entrez Gene ID: (Human) 23113
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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