Proteintech

PARD3 Polyclonal Antibody

PARD3 Antibody in Immunocytochemistry (ICC/IF)

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PARD3 Antibody (11085-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 11085-1-AP (PARD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PARD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PARD3 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

PARD3 Antibody in Immunoprecipitation (IP)

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Product Details

11085-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 11 publications 11 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Chicken, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PARD3 Fusion Protein Ag1565 (217-497 aa encoded by BC011711)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.

Immunogen sequence: RERDYAEIQ DFHRTFGCDD ELMYGGVSSY EGSMALNARP QSPREGHMMD ALYAQVKKPR NSKPSPVDSN RSTPSNHDRI QRLRQEFQQA KQDEDVEDRR RTYSFEQPWP NARPATQSGR HSVSVEVQMQ RQRQEERESS QQAQRQYSSL PRQSRKNASS VSQDSWEQNY SPGEGFQSAK ENPRYSSYQG SRNGYLGGHG FNARVMLETQ ELLRQEQRRK EQQMKKQPPS EGPSNYDSYK KVQDPSYAPP KGPFRQDVPP SPSQVARLNR LQTPEKGRPF YS (217-497 aa encoded by BC011711)

Target Information

This gene encodes a member of the kinesin 4 or subfamily of kinesin related proteins. This subfamily is also referred to as the chromokinesins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Colonic mechanism of serum NAD⁺ depletion induced by DEHP during pregnancy.

The Science of the total environment

Hong Y,Ning X,Liang YY,Li XL,Cui Y,Wu W,Cai Y,Zhao S,Zhu M,Zhong TX,Wang H,Xu DX,Xu T,Zhao LL

Wed May 10 00:00:00 UTC 2023

Par3 promotes breast cancer invasion and migration through pull tension and protein nanoparticle-induced osmotic pressure.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Hu Y,Xie Q,Chen S,Zhao W,Zhao X,Ruan Q,Zheng Z,Zhao H,Ma T,Guo J,Li L

Tue Nov 01 00:00:00 UTC 2022

Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system.

Matrix biology : journal of the International Society for Matrix Biology

Visakan G,Su J,Moradian-Oldak J

Sat Jan 01 00:00:00 UTC 2022

Polarity protein Par3 sensitizes breast cancer to paclitaxel by promoting cell cycle arrest.

Breast cancer research and treatment

Zhao Y,Peng H,Liang L,Li Y,Hu X,Wang B,Xu Y,Chen S

Tue Feb 01 00:00:00 UTC 2022

Polarity protein Par3 sensitizes breast cancer to paclitaxel by promoting cell cycle arrest.

Breast cancer research and treatment

Zhao Y,Peng H,Liang L,Li Y,Hu X,Wang B,Xu Y,Chen S

Tue Feb 01 00:00:00 UTC 2022

PARD3 gene variation as candidate cause of nonsyndromic cleft palate only.

Journal of cellular and molecular medicine

Cui R,Chen D,Li N,Cai M,Wan T,Zhang X,Zhang M,Du S,Ou H,Jiao J,Jiang N,Zhao S,Song H,Song X,Ma D,Zhang J,Li S

Mon Aug 01 00:00:00 UTC 2022

PARD3 gene variation as candidate cause of nonsyndromic cleft palate only.

Journal of cellular and molecular medicine

Cui R,Chen D,Li N,Cai M,Wan T,Zhang X,Zhang M,Du S,Ou H,Jiao J,Jiang N,Zhao S,Song H,Song X,Ma D,Zhang J,Li S

Mon Aug 01 00:00:00 UTC 2022

Effects of hydrogen peroxide on endothelial function in three-dimensional hydrogel vascular model and regulation mechanism of polar protein Par3.

Biomedical materials (Bristol, England)

Gao H,Wang B,Chen R,Jin Z,Ren L,Yang J,Wang W,Zheng N,Lin R

Wed Aug 17 00:00:00 UTC 2022

Loss of BAF (mSWI/SNF) chromatin-remodeling ATPase Brg1 causes multiple malformations of cortical development in mice.

Human molecular genetics

Jin Y,Gao X,Lu M,Chen G,Yang X,Ren N,Song Y,Hou C,Li J,Liu Q,Gao J

Mon Oct 10 00:00:00 UTC 2022

Par3/integrin β1 regulates embryo adhesion via changing endometrial luminal epithelium polarity†.

Biology of reproduction

Peng J,Li X,Zhang Y,Hu J,Shang Y,Yin Y,Xiao Z

Fri Jun 04 00:00:00 UTC 2021

EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia.

Nature communications

Kindt KS,Akturk A,Jarysta A,Day M,Beirl A,Flonard M,Tarchini B

Mon May 17 00:00:00 UTC 2021

Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells.

Cell communication and signaling : CCS

Valdivia A,Cárdenas A,Brenet M,Maldonado H,Kong M,Díaz J,Burridge K,Schneider P,San Martín A,García-Mata R,Quest AFG,Leyton L

Tue Aug 18 00:00:00 UTC 2020

Lgl1 deficiency disrupts hippocampal development and impairs cognitive performance in mice.

Genes, brain, and behavior

Zhang T,Hou C,Zhang S,Liu S,Li Z,Gao J

Fri Nov 01 00:00:00 UTC 2019

Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.

Theriogenology

Wang X,Adegoke EO,Ma M,Huang F,Zhang H,Adeniran SO,Zheng P,Zhang G

Tue Oct 15 00:00:00 UTC 2019

Coupling of apical-basal polarity and planar cell polarity to interpret the Wnt signaling gradient in feather development.

Development (Cambridge, England)

Lin J,Yue Z

Fri Sep 14 00:00:00 UTC 2018

Coupling of apical-basal polarity and planar cell polarity to interpret the Wnt signaling gradient in feather development.

Development (Cambridge, England)

Lin J,Yue Z

Fri Sep 14 00:00:00 UTC 2018

Coupling of apical-basal polarity and planar cell polarity to interpret the Wnt signaling gradient in feather development.

Development (Cambridge, England)

Lin J,Yue Z

Fri Sep 14 00:00:00 UTC 2018

Perturbation of epithelial apicobasal polarity by rhomboid family-1 gene overexpression.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Peng XM,Gao S,Deng HT,Cai HX,Zhou Z,Xiang R,Zhang QZ,Li LY

Mon Oct 01 00:00:00 UTC 2018

Perturbation of epithelial apicobasal polarity by rhomboid family-1 gene overexpression.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Peng XM,Gao S,Deng HT,Cai HX,Zhou Z,Xiang R,Zhang QZ,Li LY

Mon Oct 01 00:00:00 UTC 2018

AhR activation protects intestinal epithelial barrier function through regulation of Par-6.

Journal of molecular histology

Yu K,Ma Y,Zhang Z,Fan X,Li T,Li L,Xiao W,Cai Y,Sun L,Xu P,Yu M,Yang H

Mon Oct 01 00:00:00 UTC 2018

AhR activation protects intestinal epithelial barrier function through regulation of Par-6.

Journal of molecular histology

Yu K,Ma Y,Zhang Z,Fan X,Li T,Li L,Xiao W,Cai Y,Sun L,Xu P,Yu M,Yang H

Mon Oct 01 00:00:00 UTC 2018

[Influence of dexamethasone on the cell polarity and PAR complex of the embryonic epithelial cells in the palate].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology

Li M,Bing S,Qian Z

Thu Feb 01 00:00:00 UTC 2018

[Influence of dexamethasone on the cell polarity and PAR complex of the embryonic epithelial cells in the palate].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology

Li M,Bing S,Qian Z

Thu Feb 01 00:00:00 UTC 2018

Deletion of Brg1 causes abnormal hair cell planer polarity, hair cell anchorage, and scar formation in mouse cochlea.

Scientific reports

Jin Y,Ren N,Li S,Fu X,Sun X,Men Y,Xu Z,Zhang J,Xie Y,Xia M,Gao J

Fri Jun 03 00:00:00 UTC 2016

Deletion of Brg1 causes abnormal hair cell planer polarity, hair cell anchorage, and scar formation in mouse cochlea.

Scientific reports

Jin Y,Ren N,Li S,Fu X,Sun X,Men Y,Xu Z,Zhang J,Xie Y,Xia M,Gao J

Fri Jun 03 00:00:00 UTC 2016

Par-3 modulates intestinal epithelial barrier function through regulating intracellular trafficking of occludin and myosin light chain phosphorylation.

Journal of gastroenterology

Yu M,Yang S,Qiu Y,Chen G,Wang W,Xu C,Cai W,Sun L,Xiao W,Yang H

Sun Nov 01 00:00:00 UTC 2015

Par-3 modulates intestinal epithelial barrier function through regulating intracellular trafficking of occludin and myosin light chain phosphorylation.

Journal of gastroenterology

Yu M,Yang S,Qiu Y,Chen G,Wang W,Xu C,Cai W,Sun L,Xiao W,Yang H

Sun Nov 01 00:00:00 UTC 2015

Par-3 modulates intestinal epithelial barrier function through regulating intracellular trafficking of occludin and myosin light chain phosphorylation.

Journal of gastroenterology

Yu M,Yang S,Qiu Y,Chen G,Wang W,Xu C,Cai W,Sun L,Xiao W,Yang H

Sun Nov 01 00:00:00 UTC 2015

Par-3 modulates intestinal epithelial barrier function through regulating intracellular trafficking of occludin and myosin light chain phosphorylation.

Journal of gastroenterology

Yu M,Yang S,Qiu Y,Chen G,Wang W,Xu C,Cai W,Sun L,Xiao W,Yang H

Sun Nov 01 00:00:00 UTC 2015

Transgenic expression of constitutively active RAC1 disrupts mouse rod morphogenesis.

Investigative ophthalmology & visual science

Song H,Bush RA,Vijayasarathy C,Fariss RN,Kjellstrom S,Sieving PA

Fri Apr 25 00:00:00 UTC 2014

Transgenic expression of constitutively active RAC1 disrupts mouse rod morphogenesis.

Investigative ophthalmology & visual science

Song H,Bush RA,Vijayasarathy C,Fariss RN,Kjellstrom S,Sieving PA

Fri Apr 25 00:00:00 UTC 2014

Overexpression of EphB4 in the mammary epithelium shifts the differentiation pathway of progenitor cells and promotes branching activity and vascularization.

Development, growth & differentiation

Kaenel P,Hahnewald S,Wotzkow C,Strange R,Andres AC

Thu May 01 00:00:00 UTC 2014

Overexpression of EphB4 in the mammary epithelium shifts the differentiation pathway of progenitor cells and promotes branching activity and vascularization.

Development, growth & differentiation

Kaenel P,Hahnewald S,Wotzkow C,Strange R,Andres AC

Thu May 01 00:00:00 UTC 2014

Deregulated ephrin-B2 signaling in mammary epithelial cells alters the stem cell compartment and interferes with the epithelial differentiation pathway.

International journal of oncology

Kaenel P,Antonijevic M,Richter S,Küchler S,Sutter N,Wotzkow C,Strange R,Andres AC

Wed Feb 01 00:00:00 UTC 2012

Deregulated ephrin-B2 signaling in mammary epithelial cells alters the stem cell compartment and interferes with the epithelial differentiation pathway.

International journal of oncology

Kaenel P,Antonijevic M,Richter S,Küchler S,Sutter N,Wotzkow C,Strange R,Andres AC

Wed Feb 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: ASBP; ASIP; atypical PKC isotype-specific interacting protein; Atypical PKC isotype-specific-interacting protein; atypical PKC-specific binding protein; Atypical PKC-specific-binding protein; bazooka; CTCL tumor antigen se2-5; Ephrin-interacting protein; FLJ21015; PAR-3; par-3 (partitioning defective 3) homolog; par-3 family cell polarity regulator alpha; par-3 partitioning defective 3 homolog; PAR3-alpha; PARD-3; Partitioning defective 3 homolog; partitioning-defective 3 homolog; PHIP; protein phosphatase 1, regulatory subunit 118; RP11-406D17.1; three-PDZ containing protein similar to C. elegans PAR3 (partitioning defect)

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Gene Aliases: AA960621; AI256638; ASIP; Baz; D8Ertd580e; Par-3; PAR3; PAR3A; PAR3alpha; PARD-3; PARD3; PARD3A; Phip; PPP1R118; SE2-5L16; SE2-5LT1; SE2-5T2

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UniProt ID: (Human) Q8TEW0, (Mouse) Q99NH2, (Rat) Q9Z340

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Entrez Gene ID: (Human) 56288, (Mouse) 93742, (Rat) 81918

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Function(s)

protein binding phosphatidylinositol-4,5-bisphosphate binding phosphatidylinositol-3,4,5-trisphosphate binding protein phosphatase binding phosphatidylinositol-3-phosphate binding protein kinase C binding lipid binding

Process(es)

microtubule cytoskeleton organization apical constriction protein targeting to membrane cell cycle establishment or maintenance of cell polarity negative regulation of peptidyl-threonine phosphorylation single organismal cell-cell adhesion myelination in peripheral nervous system cell differentiation positive regulation of myelination regulation of actin filament-based process wound healing, spreading of cells cell division centrosome localization regulation of cellular localization bicellular tight junction assembly establishment of epithelial cell polarity protein complex assembly transforming growth factor beta receptor signaling pathway protein kinase C-activating G-protein coupled receptor signaling pathway axonogenesis asymmetric cell division establishment or maintenance of epithelial cell apical/basal polarity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PARD3 Polyclonal Antibody

PARD3 Antibody (11085-1-AP) in ICC/IF

Product Details

11085-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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