Invitrogen

PARN Polyclonal Antibody

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PARN Antibody (PA5-30252) in WB

Western Blot using PARN Polyclonal Antibody (Product # PA5-30252). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: BCL-1 . 7.5% SDS PAGE. PARN Polyclonal Antibody (Product # PA5-30252) diluted at 1:2,000.

Product Image Gallery

PARN Antibody — Silencing of PARN increased PLD2 protein expression. Cells were treated with transfection reagents only (Mock) or silenced with siRNA-negative control (Neg.) or with siRNA for PARN as indicated. Four days post-transfection, lysates were used for protein and gene expression analyses. (A-C) Protein expression for COS-7 cells and (F-H) for MCF-7 cells. Western blots are presented in A and F and the densitometry of PARN and PLD2 bands are shown in COS-7 cells (B-C) and MCF-7 cells (G-H). Actin was used as the equal protein loading control. (D,E) Gene expression for COS-7 cells and (I,J) for MCF-7 cells measured by RT-qPCR using the three housekeeping genes as indicated in the Fig. 1 legend. Data presented as bars are means+s.e.m. The difference between means was assessed by single-factor ANOVA. *P<0.05, significant increase between samples and controls; ##P<0.01, significant decrease between samples and controls. {KD}

Product Details

PA5-30252

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PARN. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2547726

Product Specific Information

Recommended positive controls: NIH-3T3, BCL-1.

Predicted reactivity: Mouse (87%), Rat (86%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A feedback mechanism between PLD and deadenylase PARN for the shortening of eukaryotic poly(A) mRNA tails that is deregulated in cancer cells.

Biology open

Miller TE,Gomez-Cambronero J

PA530252 was used in western blot to investigate if a signaling connection exists between phospholipase D and poly(A)-specific ribonuclease

Wed Feb 15 00:00:00 UTC 2017

A feedback mechanism between PLD and deadenylase PARN for the shortening of eukaryotic poly(A) mRNA tails that is deregulated in cancer cells.

Biology open

Miller TE,Gomez-Cambronero J

PA530252 was used in western blot to investigate if a signaling connection exists between phospholipase D and poly(A)-specific ribonuclease

Wed Feb 15 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Deadenylating nuclease; Deadenylation nuclease; Poly(A)-specific ribonuclease PARN; Polyadenylate-specific ribonuclease

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Gene Aliases: 1200003I18Rik; DAN; DKCB6; PARN; PFBMFT4

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UniProt ID: (Human) O95453, (Mouse) Q8VDG3

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Entrez Gene ID: (Human) 5073, (Mouse) 74108

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Function(s)

nucleotide binding mRNA 3'-UTR binding nuclease activity poly(A)-specific ribonuclease activity protein binding protein kinase binding poly(A) RNA binding metal ion binding nucleic acid binding RNA binding exonuclease activity hydrolase activity

Process(es)

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay nuclear-transcribed mRNA poly(A) tail shortening female gamete generation RNA modification regulation of mRNA stability RNA phosphodiester bond hydrolysis, exonucleolytic mRNA catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PARN Polyclonal Antibody

PARN Antibody (PA5-30252) in WB

Product Details

PA5-30252

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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