Invitrogen

PASK Polyclonal Antibody

PASK Antibody in Immunocytochemistry (ICC/IF)

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PASK Antibody (PA5-29309) in ICC/IF

Immunocytochemistry analysis of PASK in methanol-fixed HeLa. Samples were incubated with PASK polyclonal antibody (Product # PA5-29309) using a dilution of 1:500.

PASK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

PASK Antibody — Generation of conditional Pask alleles and deletion in beta or alpha cells. (a) Knockout (KO) strategy. Generation of beta (PaskBKO) and alpha (PaskAKO) cell-specific Pask KO mice by Cre-mediated excision of exons from 12 to 15 encoding the PASK Ser/Thr kinase domain. Human PASK cDNA (modified from [22]) with the Ser/Thr kinase domain encoding exons circled in purple and enlarged in the black box-a view from the back of PASK kinase domain (amino acids 977-1300) crystal structure (PBD code 3DLS) with the bound ADP molecule in evidence [43]. (b) Mouse Pask gene structure (modified from [22]) and the location of the loxP sites. (c) Example of a genotyping gel indicating the presence of WT and recombined (Cond) alleles. (d) Pask and Ins2 gene expression measured by qPCR in PaskBKO (BKO) and control (WT) mouse islets. Immunohistochemical analysis of pancreatic sections from PaskBKO (e) and PaskAKO (f) mice and controls, with PASK revealed using Alexa 568 (red), and insulin or glucagon revealed using Alexa 488 (green). Insets i-iv are magnified areas, as shown in the main images. Insets v-viii are example images of no-primary-antibody controls for each of the genotypes, with insets v and vii showing the DAPI staining for nuclei; scale bars, 100 µm. PASK fluorescence was displayed in 87.6 ± 1.5% of insulin-positive cells in WT islets vs 10.5 ± 2.3% in PaskBKO islets; n = 7 mice per genotype for both PaskBKO and PaskAKO mice; *p < 0.05 and **p < 0.01 by Student’s t test. Data were ob {KO}

Product Details

PA5-29309

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

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Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PASK. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2546785

Product Specific Information

Recommended positive controls: mouse brain.

Predicted reactivity: Mouse (91%), Rat (90%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PASK is an evolutionarily conserved protein present in yeast, flies, and mammals. PAS domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. It is a conserved gene product found in yeast as well as mammals. PASKIN contains two domains (PAS A and PAS B) as well as a serine/threonine kinase domain related to AMP kinases. PASKIN activity leads to decreased carbohydrate storage, as well as increased protein synthesis. PASKIN-dependent phosphorylation inhibits the activity of mammalian glycogen synthase.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cell type-specific deletion in mice reveals roles for PAS kinase in insulin and glucagon production.

Diabetologia

Semplici F,Mondragon A,Macintyre B,Madeyski-Bengston K,Persson-Kry A,Barr S,Ramne A,Marley A,McGinty J,French P,Soedling H,Yokosuka R,Gaitan J,Lang J,Migrenne-Li S,Philippe E,Herrera PL,Magnan C,da Silva Xavier G,Rutter GA

Published figure using PASK polyclonal antibody (Product # PA5-29309) in Immunofluorescence

Thu Sep 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: OTTMUSP00000024404; PAS domain-containing serine/threonine-protein kinase; PAS-kinase; paskin; per-arnt-sim (PAS) domain kinase

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Gene Aliases: KIAA0135; mKIAA0135; PASK; PASKIN; STK37

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UniProt ID: (Human) Q96RG2, (Mouse) Q8CEE6

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Entrez Gene ID: (Human) 23178, (Mouse) 269224

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Function(s)

protein serine/threonine kinase activity protein binding ATP binding phosphatidylinositol binding nucleotide binding protein kinase activity signal transducer activity lipid binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

protein phosphorylation regulation of respiratory gaseous exchange negative regulation of glycogen biosynthetic process positive regulation of translation protein autophosphorylation regulation of glucagon secretion regulation of energy homeostasis signal transduction phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PASK Polyclonal Antibody

PASK Antibody (PA5-29309) in ICC/IF

Product Details

PA5-29309

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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