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          • Primary Antibodies ›
          • PAX2 Antibodies

          Invitrogen

          PAX2a Polyclonal Antibody

          View all (38) PAX2 antibodies

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          Cite PAX2a Polyclonal Antibody

          • Antibody Testing Data (5)
          PAX2a Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
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          PAX2a Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
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          PAX2a Antibody (PA5-143393) in IHC (PFA)

          Immunohistochemistry analysis of PAX2a in 2 days-post-fertilization zebrafish embryo (whole mount). Green: Pax2a polyclonal antibody (Product # PA5-143393) dilution: 1:200. Red: Tubulin (Acetyl Lys40) antibody [6-11B-1] dilution: 1:50. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PAX2a Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          PAX2a Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          PAX2a Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          PAX2a Antibody in Western Blot (WB)
          PAX2a Antibody in Western Blot (WB)

          Product Details

          PA5-143393

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Fish, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of zebrafish Pax2a. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.21 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 20% glycerol

          Contains

          0.025% Proclin 300

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2938388

          Product Specific Information

          Store as concentrated solution.

          Centrifuge briefly prior to opening vial.

          Positive control: 2dpf zebrafish embryos, 36 hpf zebrafish embryos.

          Target Information

          The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6, which is associated with Aniridia, and pax-2, which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms' tumors. Persistent expression of Pax-2 in Wilms' tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms' tumor suppresser protein, WT1, coincides with down-regulation of the pax-2 gene; thus, suggesting that WT1 can directly repress pax-2 transcription.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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