Invitrogen

PAX3 Polyclonal Antibody

8 Published Figures

15 References

View all (29) PAX3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (8)

PAX3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

PAX3 Antibody (38-1801) in ICC/IF

Immunofluorescence analysis of PAX3 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PAX3 Rabbit Polyclonal Antibody (Product # 38-1801) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Prod... View More

PAX3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PAX3 Antibody in Immunohistochemistry (IHC)

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Product Details

38-1801

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1:250

View 6 publications 6 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

His6-tagged recombinant protein derived from a portion of the N-terminus of the mouse Pax3 sequence, encompassing the paired box and homeobox domains

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533359

Target Information

Pax 3 (Paired Box 3) is a transcription factor that is involved in development of the peripheral nervous system, melanocytes, some vascular smooth muscle, and a number of other derivatives. It regulates neurogenesis in pre-migratory neural crest cells from the dorsal neural tube, and in myogenic progenitors in the presomitic mesoderm and the hypaxial somites. Alternative splicing results in isoforms having different C-termini.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

3D Interfacial and Spatiotemporal Regulation of Human Neuroepithelial Organoids.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Tang C,Wang X,D'Urso M,van der Putten C,Kurniawan NA

Published figure using PAX3 polyclonal antibody (Product # 38-1801) in Immunocytochemistry

Mon Aug 01 00:00:00 UTC 2022

An engineered multicellular stem cell niche for the 3D derivation of human myogenic progenitors from iPSCs.

The EMBO journal

Mashinchian O,De Franceschi F,Nassiri S,Michaud J,Migliavacca E,Aouad P,Metairon S,Pruvost S,Karaz S,Fabre P,Molina T,Stuelsatz P,Hegde N,Le Moal E,Dammone G,Dumont NA,Lutolf MP,Feige JN,Bentzinger CF

38-1801 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to provide a two-week protocol for efficient and scalable suspension-based 3D derivation of Pax7-positive myogenic progenitors from hiPSCs.

Mon Jul 18 00:00:00 UTC 2022

Therapeutic potential of mesenchymal stem cells for peripheral artery disease in a rat model of hindlimb ischemia.

Iranian journal of basic medical sciences

Ali AMEA,Ahmed AS,El-Yasergy DF,Abousarie MA,Elsayed RM,Mohammed YE,Mohammed RA

38-1801 was used in Immunohistochemistry (Paraffin) to elucidate the influence of bone marrow mesenchymal stem cell transplantation on angiogenesis in a rat model of unilateral peripheral vascular disease.

Tue Jun 01 00:00:00 UTC 2021

Therapeutic potential of mesenchymal stem cells for peripheral artery disease in a rat model of hindlimb ischemia.

Iranian journal of basic medical sciences

Ali AMEA,Ahmed AS,El-Yasergy DF,Abousarie MA,Elsayed RM,Mohammed YE,Mohammed RA

Tue Jun 01 00:00:00 UTC 2021

EZH2-Mediated Primary Cilium Deconstruction Drives Metastatic Melanoma Formation.

Cancer cell

Zingg D,Debbache J,Peña-Hernández R,Antunes AT,Schaefer SM,Cheng PF,Zimmerli D,Haeusel J,Calçada RR,Tuncer E,Zhang Y,Bossart R,Wong KK,Basler K,Dummer R,Santoro R,Levesque MP,Sommer L

38-1801 was used in Immunohistochemistry to demonstrate that loss of cilia enhances pro-tumorigenic WNT/β-catenin signaling, and is itself sufficient to drive metastatic melanoma in benign cells.

Mon Jul 09 00:00:00 UTC 2018

The protective role of DOT1L in UV-induced melanomagenesis.

Nature communications

Zhu B,Chen S,Wang H,Yin C,Han C,Peng C,Liu Z,Wan L,Zhang X,Zhang J,Lian CG,Ma P,Xu ZX,Prince S,Wang T,Gao X,Shi Y,Liu D,Liu M,Wei W,Wei Z,Pan J,Wang Y,Xuan Z,Hess J,Hayward NK,Goding CR,Chen X,Zhou J,Cui R

Published figure using PAX3 polyclonal antibody (Product # 38-1801) in Immunohistochemistry

Wed Jan 17 00:00:00 UTC 2018

Loss of Ezh2 promotes a midbrain-to-forebrain identity switch by direct gene derepression and Wnt-dependent regulation.

BMC biology

Zemke M,Draganova K,Klug A,Schöler A,Zurkirchen L,Gay MH,Cheng P,Koseki H,Valenta T,Schübeler D,Basler K,Sommer L

38-1801 was used in immunohistochemistry - paraffin section to assess the Wnt-dependent regulation and midbrain-to-forebrain identity switch by loss of Ezh2

Mon Nov 30 00:00:00 UTC 2015

Recurrent PAX3-MAML3 fusion in biphenotypic sinonasal sarcoma.

Nature genetics

Wang X,Bledsoe KL,Graham RP,Asmann YW,Viswanatha DS,Lewis JE,Lewis JT,Chou MM,Yaszemski MJ,Jen J,Westendorf JJ,Oliveira AM

38-1801 was used in western blot to investigate PAX3-MAML3 fusion in biphenotypic sinonasal sarcoma

Tue Jul 01 00:00:00 UTC 2014

SOX2-LIN28/let-7 pathway regulates proliferation and neurogenesis in neural precursors.

Proceedings of the National Academy of Sciences of the United States of America

Cimadamore F,Amador-Arjona A,Chen C,Huang CT,Terskikh AV

Tue Aug 06 00:00:00 UTC 2013

Direct reprogramming of melanocytes to neural crest stem-like cells by one defined factor.

Stem cells (Dayton, Ohio)

Zabierowski SE,Baubet V,Himes B,Li L,Fukunaga-Kalabis M,Patel S,McDaid R,Guerra M,Gimotty P,Dahmane N,Herlyn M

Published figure using PAX3 polyclonal antibody (Product # 38-1801) in Immunofluorescence

Tue Nov 01 00:00:00 UTC 2011

Direct reprogramming of melanocytes to neural crest stem-like cells by one defined factor.

Stem cells (Dayton, Ohio)

Zabierowski SE,Baubet V,Himes B,Li L,Fukunaga-Kalabis M,Patel S,McDaid R,Guerra M,Gimotty P,Dahmane N,Herlyn M

Published figure using PAX3 polyclonal antibody (Product # 38-1801) in Immunofluorescence

Tue Nov 01 00:00:00 UTC 2011

Pax3-FKHR knock-in mice show developmental aberrations but do not develop tumors.

Molecular and cellular biology

Lagutina I,Conway SJ,Sublett J,Grosveld GC

Tue Oct 01 00:00:00 UTC 2002

Pax3-FKHR knock-in mice show developmental aberrations but do not develop tumors.

Molecular and cellular biology

Lagutina I,Conway SJ,Sublett J,Grosveld GC

Tue Oct 01 00:00:00 UTC 2002

The EF-hand calcium-binding protein calmyrin inhibits the transcriptional and DNA-binding activity of Pax3.

Biochimica et biophysica acta

Hollenbach AD,McPherson CJ,Lagutina I,Grosveld G

Fri Apr 12 00:00:00 UTC 2002

The oncogenic potential of the Pax3-FKHR fusion protein requires the Pax3 homeodomain recognition helix but not the Pax3 paired-box DNA binding domain.

Molecular and cellular biology

Lam PY,Sublett JE,Hollenbach AD,Roussel MF

Fri Jan 01 00:00:00 UTC 1999

The oncogenic potential of the Pax3-FKHR fusion protein requires the Pax3 homeodomain recognition helix but not the Pax3 paired-box DNA binding domain.

Molecular and cellular biology

Lam PY,Sublett JE,Hollenbach AD,Roussel MF

Fri Jan 01 00:00:00 UTC 1999

Bioinformatics

Protein Aliases: HuP2; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box gene 3; paired box homeotic gene 3; Paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; PAX3/FKHR fusion

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Gene Aliases: CDHS; HUP2; Pax-3; PAX3; Sp; splotch; WS1; WS3

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UniProt ID: (Human) P23760, (Mouse) P24610

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Entrez Gene ID: (Human) 5077, (Mouse) 18505

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Function(s)

transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding HMG box domain binding DNA binding chromatin binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding

Process(es)

transcription from RNA polymerase II promoter apoptotic process nervous system development muscle organ development sensory perception of sound organ morphogenesis neural crest formation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of transcription from RNA polymerase II promoter neural crest cell migration neural tube closure transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development heart development cell proliferation positive regulation of cell proliferation regulation of somitogenesis cell migration spinal cord association neuron differentiation neural tube development skeletal muscle cell differentiation developmental pigmentation neuron fate commitment cardiac muscle cell differentiation skeletal muscle organ development mammary gland specification

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PAX3 Polyclonal Antibody

PAX3 Antibody (38-1801) in ICC/IF

Product Details

38-1801

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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