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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PAX5 Antibodies

          Invitrogen

          PAX5 Polyclonal Antibody

          Advanced Verification
          2 Published Figures
          5 References
          View all (68) PAX5 antibodies

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          Datasheet
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          Datasheet
          Protocols
          Questions & Answers

          Cite PAX5 Polyclonal Antibody

          • Antibody Testing Data (10)
          • Published Figures (2)
          • Advanced Verification (3)
          PAX5 Antibody in Immunocytochemistry (ICC/IF)
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          PAX5 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 15

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          PAX5 Antibody (PA1-109) in ICC/IF

          Immunofluorescent analysis of PAX5 (green) in Ramos and negative control Jurkat cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 2% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a PAX5 polyclonal antibody (Product # PA1-109) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a d... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PAX5 Antibody in Immunocytochemistry (ICC/IF)
          PAX5 Antibody in Immunocytochemistry (ICC/IF)
          PAX5 Antibody in Immunocytochemistry (ICC/IF)
          PAX5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX5 Antibody in Western Blot (WB)
          PAX5 Antibody in Western Blot (WB)
          PAX5 Antibody in Western Blot (WB)
          PAX5 Antibody in Flow Cytometry (Flow)
          PAX5 Antibody in Immunoprecipitation (IP)
          PAX5 Antibody in Immunohistochemistry (IHC)
          PAX5 Antibody in Western Blot, ChIP Assay (WB, ChIP)
          PAX5 Antibody
          PAX5 Antibody
          PAX5 Antibody

          Product Details

          PA1-109

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          -

          Flow Cytometry (Flow)

          1:50
          -

          Immunoprecipitation (IP)

          2-5 µg
          -

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Purified internal fragment of human recombinant PAX5 expressed in E. coli

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS with 1mg/mL BSA, 30% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2539883

          Product Specific Information

          Western blot analysis of PA1-109 detects a prominent ~45 kDa protein in PAX5 expressing human Ramos, Daudi, Gaji and mouse A20 B-cell lymphoma cells. No PAX5 was detected in negative control Jurkat cells. The 45 kDa band is enriched in immunoprecipitations from Raji lysate, however additional unknown high molecular weight bands are also enriched and detected by PA1-109. PAX5 was also specifically detected by immunofluorescence in the nuclei of Ramos cells compared to negative control Jurkat cells.

          Target Information

          PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: B-cell lineage specific activator; B-cell-specific transcription factor; BSAP; paired box gene 5; paired box homeotic gene 5; Paired box protein Pax-5; paired domain gene 5; Pax5 variant 1; Pax5 variant 2; transcription factor PAX 5

          View more View less

          Gene Aliases: ALL3; BSAP; EBB-1; KLP; Pax-5; PAX5

          View more View less

          UniProt ID: (Human) Q02548, (Mouse) Q02650

          View more View less

          Entrez Gene ID: (Human) 5079, (Mouse) 18507

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding DNA binding transcription factor activity, sequence-specific DNA binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development spermatogenesis nervous system development aging organ morphogenesis lateral ventricle development cerebral cortex development cell differentiation adult behavior skeletal muscle cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic cranial skeleton morphogenesis negative regulation of histone H3-K9 methylation humoral immune response
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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