Invitrogen

PAX5 Polyclonal Antibody

PAX5 Antibody in Immunocytochemistry (ICC/IF)

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PAX5 Antibody (PA1-109) in ICC/IF

Immunofluorescent analysis of PAX5 (green) in Ramos and negative control Jurkat cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 2% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a PAX5 polyclonal antibody (Product # PA1-109) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a d... View More

PAX5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PAX5 Antibody in Immunoprecipitation (IP)

PAX5 Antibody in Immunohistochemistry (IHC)

PAX5 Antibody in Western Blot, ChIP Assay (WB, ChIP)

Product Image Gallery

PAX5 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lysates owing to their inherent genetic constitution. Relatively expression of PAX5 was observed in all cell lysates tested in comparison to Jurkat and Hep G2 using product (Product # PA1-109) in western blot. {RE}

Product Details

PA1-109

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

Flow Cytometry (Flow)

1:50

Immunoprecipitation (IP)

2-5 µg

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified internal fragment of human recombinant PAX5 expressed in E. coli

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2539883

Product Specific Information

Western blot analysis of PA1-109 detects a prominent ~45 kDa protein in PAX5 expressing human Ramos, Daudi, Gaji and mouse A20 B-cell lymphoma cells. No PAX5 was detected in negative control Jurkat cells. The 45 kDa band is enriched in immunoprecipitations from Raji lysate, however additional unknown high molecular weight bands are also enriched and detected by PA1-109. PAX5 was also specifically detected by immunofluorescence in the nuclei of Ramos cells compared to negative control Jurkat cells.

Target Information

PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

miR-223-3p Inhibits Human Osteosarcoma Metastasis and Progression by Directly Targeting CDH6.

Molecular therapy : the journal of the American Society of Gene Therapy

Ji Q,Xu X,Song Q,Xu Y,Tai Y,Goodman SB,Bi W,Xu M,Jiao S,Maloney WJ,Wang Y

PA1-109 was used in Western Blot to assess the role of cadherin-6 (CDH6) in human osteosarcoma, identifying that the expression of CDH6 is closely related with the overall survival and poor prognosis of osteosarcoma patients.

Wed May 02 00:00:00 UTC 2018

Cryptotanshinone Protects Cartilage against Developing Osteoarthritis through the miR-106a-5p/GLIS3 Axis.

Molecular therapy. Nucleic acids

Ji Q,Qi D,Xu X,Xu Y,Goodman SB,Kang L,Song Q,Fan Z,Maloney WJ,Wang Y

Published figure using PAX5 polyclonal antibody (Product # PA1-109) in Immunohistochemistry

Fri Jun 01 00:00:00 UTC 2018

Cryptotanshinone Protects Cartilage against Developing Osteoarthritis through the miR-106a-5p/GLIS3 Axis.

Molecular therapy. Nucleic acids

Ji Q,Qi D,Xu X,Xu Y,Goodman SB,Kang L,Song Q,Fan Z,Maloney WJ,Wang Y

Published figure using PAX5 polyclonal antibody (Product # PA1-109) in Immunohistochemistry

Fri Jun 01 00:00:00 UTC 2018

Cryptotanshinone Protects Cartilage against Developing Osteoarthritis through the miR-106a-5p/GLIS3 Axis.

Molecular therapy. Nucleic acids

Ji Q,Qi D,Xu X,Xu Y,Goodman SB,Kang L,Song Q,Fan Z,Maloney WJ,Wang Y

Published figure using PAX5 polyclonal antibody (Product # PA1-109) in Immunohistochemistry

Fri Jun 01 00:00:00 UTC 2018

Relapsed anaplastic lymphoma kinase-positive large B-cell lymphoma expressed cluster of differentiation 4 and cytokeratin: An initially misdiagnosed case corrected by immunoglobulin κ locus gene rearrangement detection.

Oncology letters

Shi Y,Li X,Song Y,Zhou L,Feng Q,Wang P,Zhang C,Liu W,Bai Y,Lai Y

PA1-109 was used in Immunohistochemistry to demonstrate breakage and extra copies of the ALK gene in ≥30% of cells.

Sat Jul 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: B-cell lineage specific activator; B-cell-specific transcription factor; BSAP; paired box gene 5; paired box homeotic gene 5; Paired box protein Pax-5; paired domain gene 5; Pax5 variant 1; Pax5 variant 2; transcription factor PAX 5

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Gene Aliases: ALL3; BSAP; EBB-1; KLP; Pax-5; PAX5

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UniProt ID: (Human) Q02548, (Mouse) Q02650

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Entrez Gene ID: (Human) 5079, (Mouse) 18507

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding DNA binding transcription factor activity, sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development spermatogenesis nervous system development aging organ morphogenesis lateral ventricle development cerebral cortex development cell differentiation adult behavior skeletal muscle cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic cranial skeleton morphogenesis negative regulation of histone H3-K9 methylation humoral immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PAX5 Polyclonal Antibody

PAX5 Antibody (PA1-109) in ICC/IF

Product Details

PA1-109

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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