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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PAX8 Antibodies

          Proteintech

          PAX8 Polyclonal Antibody

          521 References
          View all (78) PAX8 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite PAX8 Polyclonal Antibody

          • Antibody Testing Data (24)
          PAX8 Antibody in Immunocytochemistry (ICC/IF)
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          PAX8 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 24

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          PAX8 Antibody (10336-1-AP) in ICC/IF

          Confocal immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using (Product # 10336-1-AP) (PAX8 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Phalloidin stains F-actin (Red). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PAX8 Antibody in Immunocytochemistry (ICC/IF)
          PAX8 Antibody in Immunocytochemistry (ICC/IF)
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PAX8 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          PAX8 Antibody in Western Blot (WB)
          PAX8 Antibody in Western Blot (WB)
          PAX8 Antibody in Western Blot (WB)
          PAX8 Antibody in Western Blot (WB)
          PAX8 Antibody in Flow Cytometry (Flow)
          PAX8 Antibody in Immunoprecipitation (IP)
          PAX8 Antibody in Immunoprecipitation (IP)

          Product Details

          10336-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          View 49 publications 49 publications

          Immunohistochemistry (IHC)

          -
          View 340 publications 340 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:1,000-1:5,000
          -

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          1:1,200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          View 92 publications 92 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          View 1 publication 1 publication

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          3 µg
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 15 publications 15 publications

          Not applicable (N/A)

          -
          View 22 publications 22 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Bovine, Human, Mouse, Rat, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          PAX8 Fusion Protein Ag0306

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.23 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa, and 48 kDa). A 58-60 kDa form has also been reported. Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.

          Target Information

          This gene encodes a member of the paired box family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: HGNC:8622; paired box gene 8; Paired box protein Pax-8; paired domain gene 8; Pax-8 protein

          View more View less

          Gene Aliases: Pax-8; PAX8

          View more View less

          UniProt ID: (Human) Q06710, (Mouse) Q00288, (Rat) P51974

          View more View less

          Entrez Gene ID: (Human) 7849, (Mouse) 18510, (Rat) 81819

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding thyroid-stimulating hormone receptor activity protein binding transcription regulatory region DNA binding sequence-specific DNA binding
          Process(es)
          urogenital system development metanephros development mesonephros development transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development organ morphogenesis cell differentiation thyroid gland development inner ear morphogenesis regulation of apoptotic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter pronephros development cellular response to gonadotropin stimulus positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis metanephric distal convoluted tubule development metanephric nephron tubule formation negative regulation of mesenchymal cell apoptotic process involved in metanephric nephron morphogenesis regulation of metanephric nephron tubule epithelial cell differentiation positive regulation of branching involved in ureteric bud morphogenesis negative regulation of mesenchymal cell apoptotic process involved in metanephros development negative regulation of apoptotic process involved in metanephric collecting duct development negative regulation of apoptotic process involved in metanephric nephron tubule development positive regulation of metanephric DCT cell differentiation positive regulation of thyroid hormone generation regulation of thyroid-stimulating hormone secretion branching involved in ureteric bud morphogenesis kidney development mesenchymal to epithelial transition involved in metanephros morphogenesis sulfur compound metabolic process central nervous system development anatomical structure morphogenesis thyroid-stimulating hormone signaling pathway pronephric field specification otic vesicle development metanephric epithelium development metanephric comma-shaped body morphogenesis metanephric S-shaped body morphogenesis
          It has to be done as per old AB suggested Products section.
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