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FIGURE: 1 / 2
PBRM1 Antibody (PA5-97280) in WB
Western blot analysis of PBRM1 in extracts of various cell lines. Samples were incubated with PBRM1 Polyclonal antibody (Product # PA5-97280) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Product Details
PA5-97280
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human PBRM1 (NP_0607833)
Conjugate
Form
Liquid
Concentration
1.03 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2809082
Product Specific Information
Immunogen sequence: MGSKRRRATS PSSSVSGDFD DGHHSVSTPG PSRKRRRLSN LPTVDPIAVC HELYNTIRDY KDEQGRLLCE LFIRAPKRRN QPDYYEVVSQ PIDLMKIQQK LKMEEYDDVN LLTADFQLLF NNAKSYYKPD SPEYKAACKL WDLYLRTRNE FVQKGEADDE DDDEDGQDNQ GTVTEGSSPA YLKEILEQLL EAIVVATNPS GRLISELFQK LPSKVQYPDY YAIIKEPIDL; Positive Samples: HeLa, Jurkat, BT-474, NIH/3T3, LO2, Mouse lung, Mouse thymus, Rat liver; Cellular Location: Nucleus
Target Information
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2310032M22Rik; BAF180; BRG1-associated factor 180; hPB1; polybromo 1; Polybromo-1D; Protein polybromo-1
Gene Aliases: 2610016F04Rik; AI507524; BAF180; PB1; PBRM1; RGD1565549
UniProt ID: (Human) Q86U86
Entrez Gene ID: (Human) 55193, (Rat) 306254, (Mouse) 66923
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
mitotic nuclear division
negative regulation of cell proliferation
covalent chromatin modification
placenta development
heart development
biological_process
blood vessel remodeling
heart morphogenesis
epicardium-derived cardiac endothelial cell differentiation
chromatin modification
positive regulation of transcription, DNA-templated
cardiac vascular smooth muscle cell development
vasculogenesis involved in coronary vascular morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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