Invitrogen

PBX1 Polyclonal Antibody

Advanced Verification

1 Published Figure

View all (30) PBX1 antibodies

Questions & Answers

Questions & Answers

PBX1 Antibody in Immunocytochemistry (ICC/IF)

PBX1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

PBX1 Antibody (PA5-82100) in ICC/IF

Immunofluorecent analysis of PBX1 in human cell line U-251 MG using PBX1 Polyclonal Antibody (Product # PA5-82100). Staining shows localization to nucleoplasm.

PBX1 Antibody in Immunocytochemistry (ICC/IF)

PBX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PBX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PBX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PBX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PBX1 Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation (WB, ICC/IF, IHC (P), IP)

Product Image Gallery

PBX1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorecent analysis of PBX1 in human cell line U-251 MG using PBX1 Polyclonal Antibody (Product # PA5-82100). Staining shows localization to nucleoplasm.

PBX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PBX1 in human endometrium using PBX1 Polyclonal Antibody (Product # PA5-82100) shows moderate to strong nuclear positivity in stromal cells.

PBX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PBX1 in human prostate using PBX1 Polyclonal Antibody (Product # PA5-82100) shows weak to moderate nuclear positivity in glandular cells.

PBX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PBX1 in human fallopian tube using PBX1 Polyclonal Antibody (Product # PA5-82100) shows strong nuclear positivity in glandular cells.

PBX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PBX1 in human liver using PBX1 Polyclonal Antibody (Product # PA5-82100) shows no positivity in hepatocytes as expected.

PBX1 Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation (WB, ICC/IF, IHC (P), IP) — Fig. 4 Interaction candidates are coexpressed with TBX2 in the pulmonary mesenchyme and interact in HEK293 cells. a Co-immunofluorescence analysis of candidate interaction partners (red) and TBX2 (green) on frontal sections of the right lung of E14.5 Tbx2 cre/ + embryos. Antigens are color-coded and nuclei were counterstained with DAPI (blue). Insets or selected regions in overview images are magnified in rows 2,4 and 6. b In situ proximity ligation assay of TBX2 and candidate interaction partners on 10 um frontal sections of E14.5 wildtype and Tbx2 cre/fl mutant lungs. Direct interaction is visualized by small red fluorescent dots. Larger more diffuse orange stains are due to auto-fluorescence of blood cells. Nuclei are counterstained with DAPI (blue). c Western blot analysis of co-immunoprecipitation experiments for verification of TBX2 interaction with candidate proteins on 10% SDS polyacrylamide gels. Detection was performed with an anti-TBX2 primary antibody and developed with chemoluminescence-IHC. Arrows indicate TBX2 bands. Lanes were loaded as follows: No antibody: IP without specific antibody resembling negative IP-control; 5% input: 5% of crude cell extract before precipitation; empty: no protein loaded; IP: co-immunoprecipitate with antibody for specific candidate. Expected molecular weight for TBX2.HA approx. 76.2 kDa

PBX1 Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of PBX1 was observed specifically in HeLa cells and in SK-MEL-30 cells using anti-PBX1 Polyclonal Antibody (Product #PA5-82100) in western blot. The relative expression levels of PBX1 within each cell line is shown using RNA-Seq. {RE}

Product Details

PA5-82100

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PBX1. Recombinant protein control fragment (Product #RP-101910).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2789261

Product Specific Information

Immunogen sequence: PRLMHSHAGV GMAGHPGLSQ HLQDGAGGTE GEGGRKQDIG DILQQIMTIT DQSLDEAQAR KHALNCHRMK PALFNVLCEI KEKTVLSIRG AQEEEPTDPQ LMRLDNMLLA EGV

Target Information

This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis, and required for skeletal patterning and programming. A chromosomal translocation, t(1; 19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

PA5-82100 was used in Immunoprecipitation, Immunohistochemistry (Paraffin) to suggest that TBX2 interacts with homeobox and HMG-box transcription factors as well as with the NuRD chromatin remodeling complex to repress transcription of anti-proliferative genes in the pulmonary mesenchyme.

Wed Mar 17 00:00:00 UTC 2021

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

PA5-82100 was used in Immunoprecipitation, Immunohistochemistry (Paraffin) to suggest that TBX2 interacts with homeobox and HMG-box transcription factors as well as with the NuRD chromatin remodeling complex to repress transcription of anti-proliferative genes in the pulmonary mesenchyme.

Wed Mar 17 00:00:00 UTC 2021

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

PA5-82100 was used in Immunoprecipitation, Immunohistochemistry (Paraffin) to suggest that TBX2 interacts with homeobox and HMG-box transcription factors as well as with the NuRD chromatin remodeling complex to repress transcription of anti-proliferative genes in the pulmonary mesenchyme.

Wed Mar 17 00:00:00 UTC 2021

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

PA5-82100 was used in Immunoprecipitation, Immunohistochemistry (Paraffin) to suggest that TBX2 interacts with homeobox and HMG-box transcription factors as well as with the NuRD chromatin remodeling complex to repress transcription of anti-proliferative genes in the pulmonary mesenchyme.

Wed Mar 17 00:00:00 UTC 2021

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

PA5-82100 was used in Immunoprecipitation, Immunohistochemistry (Paraffin) to suggest that TBX2 interacts with homeobox and HMG-box transcription factors as well as with the NuRD chromatin remodeling complex to repress transcription of anti-proliferative genes in the pulmonary mesenchyme.

Wed Mar 17 00:00:00 UTC 2021

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

PA5-82100 was used in Immunoprecipitation, Immunohistochemistry (Paraffin) to suggest that TBX2 interacts with homeobox and HMG-box transcription factors as well as with the NuRD chromatin remodeling complex to repress transcription of anti-proliferative genes in the pulmonary mesenchyme.

Wed Mar 17 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: DKFZp686B09108; Homeobox protein PBX1; Homeobox protein PRL; MGC126627; OTTHUMP00000032205; pre-B-cell leukemia homeobox 1; Pre-B-cell leukemia transcription factor 1

View more View less

Gene Aliases: 2310056B04Rik; D230003C07Rik; Pbx-1; PBX1; PRL

View more View less

UniProt ID: (Human) P40424, (Mouse) P41778

View more View less

Entrez Gene ID: (Human) 5087, (Mouse) 18514, (Rat) 304947

View more View less

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein heterodimerization activity transcription factor binding sequence-specific DNA binding molecular_function

urogenital system development branching involved in ureteric bud morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter steroid biosynthetic process multicellular organism development sex differentiation positive regulation of cell proliferation organ morphogenesis anterior/posterior pattern specification proximal/distal pattern formation positive regulation of G2/M transition of mitotic cell cycle cell differentiation regulation of ossification adrenal gland development embryonic limb morphogenesis embryonic hemopoiesis regulation of cell proliferation negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter spleen development thymus development embryonic organ development embryonic skeletal system development somatic stem cell population maintenance biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support