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PBX1 Antibody (MA5-50794) in IHC (P)
Immunohistochemistry of PBX1 in paraffin-embedded mouse pancreas tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with PBX1 recombinant monoclonal antibody (Product # MA5-50794) with a dilution of 1:200 for 1 hour at roo... View More
Product Details
MA5-50794
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH0-24
Immunogen
Recombinant protein within human PBX1 aa 1-250/430.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3093792
Product Specific Information
Positive control: Hela cell lysate, HEK-293 cell lysate, MCF-7 cell lysate, Jurkat cell lysate, A431 cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate, rat pancreas tissue lysate, human pancreas tissue, mouse pancreas tissue.
Predicted band size: 47 kDa
Subcellular Location: Nucleus.
Target Information
This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis, and required for skeletal patterning and programming. A chromosomal translocation, t(1; 19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686B09108; Homeobox protein PBX1; Homeobox protein PRL; MGC126627; OTTHUMP00000032205; pre-B-cell leukemia homeobox 1; Pre-B-cell leukemia transcription factor 1
Gene Aliases: 2310056B04Rik; D230003C07Rik; Pbx-1; PBX1; PRL
UniProt ID: (Human) P40424, (Mouse) P41778
Entrez Gene ID: (Human) 5087, (Mouse) 18514, (Rat) 304947
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein heterodimerization activity
transcription factor binding
sequence-specific DNA binding
molecular_function
urogenital system development
branching involved in ureteric bud morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
steroid biosynthetic process
multicellular organism development
sex differentiation
positive regulation of cell proliferation
organ morphogenesis
anterior/posterior pattern specification
proximal/distal pattern formation
positive regulation of G2/M transition of mitotic cell cycle
cell differentiation
regulation of ossification
adrenal gland development
embryonic limb morphogenesis
embryonic hemopoiesis
regulation of cell proliferation
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
spleen development
thymus development
embryonic organ development
embryonic skeletal system development
somatic stem cell population maintenance
biological_process
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