Invitrogen

PCAF Monoclonal Antibody (7D12C8)

View all (31) PCAF antibodies

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-38547

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000
-

Immunocytochemistry (ICC/IF)

1:200-1:1,000
-

Flow Cytometry (Flow)

1:200-1:400
-

ELISA (ELISA)

1:10,000
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7D12C8

Immunogen

Purified recombinant fragment of human KAT2B (aa: 1-142) expressed in E. Coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2898459

Product Specific Information

This antibody has been tested in indirect ELISA.

Target Information

PCAF (histone acetyltransferase KAT2B) functions as a histone acetyltransferase (HAT) to promote transcriptional activation. It has significant histone acetyltransferase activity with core histones (H3 and H4) and nucleosome core particles. PCAF also acetylates non-histone proteins such as ACLY, PLK4, and TBX5. It inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. PCAF also acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It is involved in heart and limb development by mediating acetylation of TBX5 and acetylation regulating nucleocytoplasmic shutting of TBX5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: CREBBP-associated factor; Histone acetylase PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; K(lysine) acetyltransferase 2B; Lysine acetyltransferase 2B; P/ PCAF; P/CAF; P300/CBP-associated factor; Spermidine acetyltransferase KAT2B

View more View less

Gene Aliases: A930006P13Rik; AI461839; AW536563; CAF; KAT2B; P/CAF; PCAF

View more View less

UniProt ID: (Human) Q92831, (Mouse) Q9JHD1

View more View less

Entrez Gene ID: (Human) 8850, (Rat) 301164, (Mouse) 18519

View more View less

Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding chromatin binding transcription cofactor activity transcription coactivator activity histone acetyltransferase activity lysine N-acetyltransferase activity, acting on acetyl phosphate as donor cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding transcription factor binding acetyltransferase activity protein kinase binding protein complex binding histone acetyltransferase binding histone deacetylase binding N-acetyltransferase activity transferase activity transferase activity, transferring acyl groups
Process(es)
chromatin remodeling protein acetylation memory negative regulation of cell proliferation regulation of protein ADP-ribosylation positive regulation of neuron projection development histone acetylation N-terminal peptidyl-lysine acetylation internal peptidyl-lysine acetylation peptidyl-lysine acetylation cellular response to insulin stimulus cellular response to oxidative stress positive regulation of chromatin binding positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter histone H3 acetylation histone H3-K9 acetylation negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of transcription, DNA-templated positive regulation of vasodilation positive regulation of transcription from RNA polymerase II promoter cellular response to parathyroid hormone stimulus positive regulation of histone H3-K14 acetylation positive regulation of histone H3-K9 acetylation transcription initiation from RNA polymerase II promoter cell cycle arrest Notch signaling pathway viral process positive regulation of gene expression, epigenetic rhythmic process transcription, DNA-templated regulation of transcription, DNA-templated cell cycle
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support