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PCBP2 Polyclonal Antibody

PCBP2 Antibody in Immunocytochemistry (ICC/IF)

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PCBP2 Antibody (15070-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 15070-1-AP (PCBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PCBP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

15070-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PCBP2 Fusion Protein Ag7102 (1-362 aa encoded by BC001155)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against a full-length human PCBP2 protein.

Immunogen sequence: MDTGVIEGG LNVTLTIRLL MHGKEVGSII GKKGESVKKM REESGARINI SEGNCPERII TLAGPTNAIF KAFAMIIDKL EEDISSSMTN STAASRPPVT LRLVVPASQC GSLIGKGGCK IKEIRESTGA QVQVAGDMLP NSTERAITIA GIPQSIIECV KQICVVMLES PPKGVTIPYR PKPSSSPVIF AGGQDRYSTG SDSASFPHTT PSMCLNPDLE GPPLEAYTIQ GQYAIPQPDL TKLHQLAMQQ SHFPMTHGNT GFSGIESSSP EVKGYWAGLD ASAQTTSHEL TIPNDLIGCI IGRQGAKINE IRQMSGAQIK IANPVEGSTD RQVTITGSAA SISLAQYLIN VRLSSETGGM GSS (1-362 aa encoded by BC001155)

Target Information

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human hematopoietic stem cell vulnerability to ferroptosis.

Cell

Zhao J,Jia Y,Mahmut D,Deik AA,Jeanfavre S,Clish CB,Sankaran VG

Thu Feb 16 00:00:00 UTC 2023

METTL3 preferentially enhances non-m⁶A translation of epigenetic factors and promotes tumourigenesis.

Nature cell biology

Wei X,Huo Y,Pi J,Gao Y,Rao S,He M,Wei Q,Song P,Chen Y,Lu D,Song W,Liang J,Xu L,Wang H,Hong G,Guo Y,Si Y,Xu J,Wang X,Ma Y,Yu S,Zou D,Jin J,Wang F,Yu J

Mon Aug 01 00:00:00 UTC 2022

N4-acetylcytidine regulates the replication and pathogenicity of enterovirus 71.

Nucleic acids research

Hao H,Liu W,Miao Y,Ma L,Yu B,Liu L,Yang C,Zhang K,Chen Z,Yang J,Zheng Z,Zhang B,Deng F,Gong P,Yuan J,Hu Z,Guan W

Fri Sep 09 00:00:00 UTC 2022

PCBP2 maintains antiviral signaling homeostasis by regulating cGAS enzymatic activity via antagonizing its condensation.

Nature communications

Gu H,Yang J,Zhang J,Song Y,Zhang Y,Xu P,Zhu Y,Wang L,Zhang P,Li L,Chen D,Sun Q

Wed Mar 23 00:00:00 UTC 2022

PCBP2 maintains antiviral signaling homeostasis by regulating cGAS enzymatic activity via antagonizing its condensation.

Nature communications

Gu H,Yang J,Zhang J,Song Y,Zhang Y,Xu P,Zhu Y,Wang L,Zhang P,Li L,Chen D,Sun Q

Wed Mar 23 00:00:00 UTC 2022

Ectopic Expression of TRIM25 Restores RIG-I Expression and IFN Production Reduced by Multiple Enteroviruses 3C^pro.

Virologica Sinica

Xiao H,Li J,Yang X,Li Z,Wang Y,Rui Y,Liu B,Zhang W

Wed Dec 01 00:00:00 UTC 2021

PCBP2 Posttranscriptional Modifications Induce Breast Cancer Progression via Upregulation of UFD1 and NT5E.

Molecular cancer research : MCR

Wang X,Guo Q,Wang H,Yuan X,Wang B,Lobie PE,Zhu T,Tan S,Wu Z

Fri Jan 01 00:00:00 UTC 2021

PCBP2 Posttranscriptional Modifications Induce Breast Cancer Progression via Upregulation of UFD1 and NT5E.

Molecular cancer research : MCR

Wang X,Guo Q,Wang H,Yuan X,Wang B,Lobie PE,Zhu T,Tan S,Wu Z

Fri Jan 01 00:00:00 UTC 2021

Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication.

Veterinary microbiology

Zhang P,Yu L,Dong J,Liu Y,Zhang L,Liang P,Wang L,Chen B,Huang L,Song C

Sat Aug 01 00:00:00 UTC 2020

Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication.

Veterinary microbiology

Zhang P,Yu L,Dong J,Liu Y,Zhang L,Liang P,Wang L,Chen B,Huang L,Song C

Sat Aug 01 00:00:00 UTC 2020

Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2.

FEBS open bio

Chen C,Lei J,Zheng Q,Tan S,Ding K,Yu C

Tue May 01 00:00:00 UTC 2018

Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2.

FEBS open bio

Chen C,Lei J,Zheng Q,Tan S,Ding K,Yu C

Tue May 01 00:00:00 UTC 2018

Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2.

FEBS open bio

Chen C,Lei J,Zheng Q,Tan S,Ding K,Yu C

Tue May 01 00:00:00 UTC 2018

Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2.

FEBS open bio

Chen C,Lei J,Zheng Q,Tan S,Ding K,Yu C

Tue May 01 00:00:00 UTC 2018

Spatiotemporal Expression of Poly(rC)-Binding Protein PCBP2 Modulates Schwann Cell Proliferation After Sciatic Nerve Injury.

Cellular and molecular neurobiology

Chen Z,Zhang W,Ni L,Wang G,Cao Y,Wu W,Sun C,Yuan D,Ni H,Wang Y,Yang H

Fri Jul 01 00:00:00 UTC 2016

Spatiotemporal Expression of Poly(rC)-Binding Protein PCBP2 Modulates Schwann Cell Proliferation After Sciatic Nerve Injury.

Cellular and molecular neurobiology

Chen Z,Zhang W,Ni L,Wang G,Cao Y,Wu W,Sun C,Yuan D,Ni H,Wang Y,Yang H

Fri Jul 01 00:00:00 UTC 2016

Spatiotemporal Expression of Poly(rC)-Binding Protein PCBP2 Modulates Schwann Cell Proliferation After Sciatic Nerve Injury.

Cellular and molecular neurobiology

Chen Z,Zhang W,Ni L,Wang G,Cao Y,Wu W,Sun C,Yuan D,Ni H,Wang Y,Yang H

Fri Jul 01 00:00:00 UTC 2016

Spatiotemporal Expression of Poly(rC)-Binding Protein PCBP2 Modulates Schwann Cell Proliferation After Sciatic Nerve Injury.

Cellular and molecular neurobiology

Chen Z,Zhang W,Ni L,Wang G,Cao Y,Wu W,Sun C,Yuan D,Ni H,Wang Y,Yang H

Fri Jul 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Alpha CP2; Alpha-CP2; Heterogeneous nuclear ribonucleoprotein E2; heterogenous nuclear ribonucleoprotein E2; hnRNP E2; MGC110998; Poly(rC)-binding protein 2; rCbinding protein 2

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Gene Aliases: hnRNP-E2; HNRNPE2; HNRPE2; PCBP2

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UniProt ID: (Human) Q15366

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Entrez Gene ID: (Human) 5094

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Function(s)

DNA binding RNA binding protein binding enzyme binding ubiquitin protein ligase binding poly(A) RNA binding

Process(es)

mRNA splicing, via spliceosome gene expression mRNA metabolic process negative regulation of type I interferon production viral RNA genome replication proteasome-mediated ubiquitin-dependent protein catabolic process innate immune response negative regulation of defense response to virus defense response to virus IRES-dependent viral translational initiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PCBP2 Polyclonal Antibody

PCBP2 Antibody (15070-1-AP) in ICC/IF

Product Details

15070-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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