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Invitrogen
PCDH1 (Protocadherin 1) Monoclonal Antibody (CSTEM31), eBioscience™
FIGURE: 1 / 2
PCDH1 (Protocadherin 1) Antibody (14-9157-82) in ICC/IF
Immunocytochemistry of fixed and permeabilized human iPSCs plated on murine derived feeder cells using 5 µg/mL Mouse IgG2b K Isotype Control (Product # 14-4732-82) (left) or 5 µg/mL Anti-Human PCDH1 (Protocadherin 1) purified (right) followed by F(ab’)2-Goat anti-Mouse IgG Secondary Antibody eFluor® 660 (Product # 50-4010-82). Nuclei are stained with DAPI.
Product Details
14-9157-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
CSTEM31
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with proprietary stabilizer
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2716937
Product Specific Information
This monoclonal antibody CSTEM31 reacts with human Protocadherin-1, also known as PCDH1 and cadherin-like protein 1.
Applications Reported: This CSTEM31 antibody has been reported for use in immunocytochemical staining and flow cytometric analysis. (Fluorochrome-conjugated CSTEM31 is recommended for use in flow cytometery.)
Applications Tested: This CSTEM31 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized human iPSCs, and by flow cytometry on human iPSCs. For immunocytochemistry, this can be used at less than or equal to 5 µg/mL. For flow cytometry, this antibody can be used at less than or equal to 0.5 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Target Information
Protocadherin 1, also known as PCDH1 and cadherin-like protein 1, is a transmembrane protein involved in cell-cell adhesion. Protocadherin 1 belongs to a group of cadherin-like proteins termed delta1-protocadherins, which consists of protocadherin-1, -7, -9, and -11(X/Y). The delta1-protocadherins have seven extracellular cadherin-repeats, a transmembrane domain, and a cytoplasmic domain, and can be expressed in two isoforms, short or long. Expression of protocadherin 1 is regulated both temporally and spatially, and plays a role both in early development and mature tissues. In mice, it is expressed in a number of tissues and organs, including the brain, spinal cord, peripheral ganglia, inner ear, hair follicles, kidney, reproductive organs, prostate, and the seminal gland. Notably, protocadherin 1 is a susceptibility gene for Bronchial Hyperresponsiveness in humans. Expression of protocadherin 1 has also been identified on human pluripotent stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cadherin-like 1; Cadherin-like protein 1; PC42; protocadherin 42; Protocadherin-1; Protocadherin-42
Gene Aliases: PC42; PCDH1; PCDH42
UniProt ID: (Human) Q08174
Entrez Gene ID: (Human) 5097
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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