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PCDH15 Antibody (CF812881) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCDH15 (RC226513, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PCDH1. Positive lysates LY428262 (100 µg) and LC428262 (20 µg) can be purchased separately from OriGene.
Product Details
CF812881
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI7F10
Immunogen
Human recombinant protein fragment corresponding to amino acids 160-400 of human PCDH15 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
PCDH15 is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. PCDH15 consists of a signal peptide, 11 extracellular calcium-binding domains, a transmembrane domain and a unique cytoplasmic domain. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene have been associated with hearing loss, which is consistent with its location at the Usher syndrome type 1F (USH1F) critical region on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ames waltzer; cadherin-related family member 15; DKFZp667A1711; protocadherin 15 CD2; protocadherin 15 CD3 isoform; Protocadherin-15; protocadherin-related 15; RP11-449J3.2
Gene Aliases: av; BB078305; CDHR15; DFNB23; ENSMUSG00000046980; Gm9815; nmf19; PCDH15; USH1F
UniProt ID: (Human) Q96QU1, (Mouse) Q99PJ1
Entrez Gene ID: (Human) 65217, (Rat) 690865, (Mouse) 11994
homophilic cell adhesion via plasma membrane adhesion molecules
sensory perception of sound
photoreceptor cell maintenance
inner ear development
sensory perception of light stimulus
equilibrioception
startle response
morphogenesis of an epithelium
actin filament organization
visual perception
locomotory behavior
adult walking behavior
adult locomotory behavior
nonmotile primary cilium assembly
multicellular organism growth
auditory receptor cell differentiation
detection of mechanical stimulus involved in sensory perception of sound
detection of mechanical stimulus involved in equilibrioception
response to calcium ion
righting reflex
auditory receptor cell stereocilium organization
cell adhesion
inner ear receptor stereocilium organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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