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PCDHA12 Antibody (PA5-97090) in WB
Western blot analysis of PCDHA12 in extracts of various cell lines. Samples were incubated with PCDHA12 Polyclonal antibody (Product # PA5-97090) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-97090
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 713-792 of human PCDHA12 (NP_1140701)
Conjugate
Form
Liquid
Concentration
0.69 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808892
Product Specific Information
Immunogen sequence: LTLLLYTALR CSAPPTVSRC APGKPTLVCS SAVGSWSYSQ QRRQRVCSAE SPPKTDLMAF SPSLQLSRED CLNPPSEVSY; Positive Samples: HL-60, H460, NIH/3T3, Mouse spinal cord, Mouse brain, Mouse lung, Rat spinal cord, Rat brain; Cellular Location: Cell membrane, Single-pass type I membrane protein
Target Information
This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cadherin-related neuronal receptor 5; KIAA0345-like 2; MGC138485; MGC141932; PCDH-alpha-12; protocadherin alpha 12; protocadherin alpha 13; Protocadherin alpha-12
Gene Aliases: Cnr5; Crnr5; PCDH-ALPHA12; PCDHA12; Pcdha13; rCNRv12
UniProt ID: (Human) Q9UN75
Entrez Gene ID: (Human) 56137, (Rat) 116779, (Mouse) 192164
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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