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PCIF1 Antibody (PA5-110081) in WB
Western blot analysis of PCIF1 in HeLa cells. Samples were incubated with PCIF1 Polyclonal antibody (Product # PA5-110081) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
Product Details
PA5-110081
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PCIF1 (NP_0713871)
Conjugate
Form
Liquid
Concentration
2.47 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2855492
Product Specific Information
Immunogen sequence: MANENHGSPR EEASLLSHSP GTSNQSQPCS PKPIRLVQDL PEELVHAGWE KCWSRRENRP YYFNRFTNQS LWEMPVLGQH DVISDPLGLN ATPLPQDSSL VETPPAENKP RKRQLSEEQP SGNGVKKPKI EIPVTPTGQS VPSSPSIPGT PTLKMWGTSP EDKQQAALLR PTEVYWDLDI QTNAVIKHRG PSEVLPPHPE
Target Information
PCIF1 may play a role in transcription elongation or in coupling transcription to pre-mRNA processing through its association with the phosphorylated C-terminal domain (CTD) of RNAPII largest subunit.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cap-specific adenosine methyltransferase; CAPAM; hPCIF1; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; PDX-1 C terminus-interacting factor 1; phosphorylated CTD interacting factor PCIF1; Phosphorylated CTD-interacting factor 1; Protein phosphatase 1 regulatory subunit 121; protein phosphatase 1, regulatory subunit 121
Gene Aliases: 2310022K11Rik; C20orf67; CAPAM; F730014I05Rik; PCIF1; PPP1R121; RGD1310800
UniProt ID: (Human) Q9H4Z3, (Mouse) P59114
Entrez Gene ID: (Human) 63935, (Mouse) 228866, (Rat) 362269
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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