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PCK2 Polyclonal Antibody

PCK2 Antibody in Immunocytochemistry (ICC/IF)

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PCK2 Antibody (14892-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using (Product # 14892-1-AP) (PCK2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PCK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

14892-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PCK2 Fusion Protein Ag6681 (292-640 aa encoded by BC001454)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: AGKKRYVAA AFPSACGKTN LAMMRPALPG WKVECVGDDI AWMRFDSEGR LRAINPENGF FGVAPGTSAT TNPNAMATIQ SNTIFTNVAE TSDGGVYWEG IDQPLPPGVT VTSWLGKPWK PGDKEPCAHP NSRFCAPARQ CPIMDPAWEA PEGVPIDAII FGGRRPKGVP LVYEAFNWRH GVFVGSAMRS ESTAAAEHKG KIIMHDPFAM RPFFGYNFGH YLEHWLSMEG RKGAQLPRIF HVNWFRRDEA GHFLWPGFGE NARVLDWICR RLEGEDSARE TPIGLVPKEG ALDLSGLRAI DTTQLFSLPK DFWEQEVRDI RSYLTEQVNQ DLPKEVLAEL EALERRVHKM (292-640 aa encoded by BC001454)

Target Information

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exercise postconditioning reduces ischemic injury via suppression of cerebral gluconeogenesis in rats.

Brain and behavior

Li F,Geng X,Ilagan R,Bai S,Chen Y,Ding Y

Sun Jan 01 00:00:00 UTC 2023

Exercise postconditioning reduces ischemic injury via suppression of cerebral gluconeogenesis in rats.

Brain and behavior

Li F,Geng X,Ilagan R,Bai S,Chen Y,Ding Y

Sun Jan 01 00:00:00 UTC 2023

Douchi Peptides VY and SFLLR Improve Glucose Homeostasis and Gut Dysbacteriosis in High-Fat Diet-Induced Insulin Resistant Mice.

Molecular nutrition & food research

Yu S,Wang W,Wang H,Bu T,Liu L,Zhao R,Li S,Liu D,Wu J

Mon May 01 00:00:00 UTC 2023

MiR-34a-5p promotes hepatic gluconeogenesis by suppressing SIRT1 expression.

Experimental cell research

Wang Y,Zhou F,Li M,Zhang Y,Li N,Shao L

Tue Nov 01 00:00:00 UTC 2022

Different types of reactions to E7386 among colorectal cancer patient-derived organoids and corresponding CAFs.

Oncology letters

Imai T,Naruse M,Ochiai M,Matsumoto K,Ikeda S,Kani M,Kato Y,Hirayama A,Soga T,Hori Y,Yokoi A,Ochiai A

Fri Jul 01 00:00:00 UTC 2022

CHCHD2 and CHCHD10 regulate mitochondrial dynamics and integrated stress response.

Cell death & disease

Ruan Y,Hu J,Che Y,Liu Y,Luo Z,Cheng J,Han Q,He H,Zhou Q

Wed Feb 16 00:00:00 UTC 2022

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues.

Journal of immunology (Baltimore, Md. : 1950)

Fan TW,Daneshmandi S,Cassel TA,Uddin MB,Sledziona J,Thompson PT,Lin P,Higashi RM,Lane AN

Tue Nov 01 00:00:00 UTC 2022

Phosphoenolpyruvate Carboxykinase (PCK) in the Brain Gluconeogenic Pathway Contributes to Oxidative and Lactic Injury After Stroke.

Molecular neurobiology

Geng X,Shen J,Li F,Yip J,Guan L,Rajah G,Peng C,DeGracia D,Ding Y

Sat May 01 00:00:00 UTC 2021

Bicyclol Regulates Hepatic Gluconeogenesis in Rats with Type 2 Diabetes and Non-alcoholic Fatty Liver Disease by Inhibiting Inflammation.

Frontiers in pharmacology

Li H,Xu Q,Xu C,Hu Y,Yu X,Zhao K,Li M,Li M,Xu J,Kuang H

Tue Jun 08 00:00:00 UTC 2021

Human umbilical cord-derived mesenchymal stem cell-exosomal miR-627-5p ameliorates non-alcoholic fatty liver disease by repressing FTO expression.

Human cell

Cheng L,Yu P,Li F,Jiang X,Jiao X,Shen Y,Lai X

Mon Nov 01 00:00:00 UTC 2021

Down-regulation of PCK2 inhibits the invasion and metastasis of laryngeal carcinoma cells.

American journal of translational research

Hu Y,Deng K,Pan M,Liu S,Li W,Huang J,Yao J,Zuo J

Wed Jul 31 00:00:00 UTC 2024

Inhibition of Anaplerosis Attenuated Vascular Proliferation in Pulmonary Arterial Hypertension.

Journal of clinical medicine

Valuparampil Varghese M,James J,Eccles CA,Niihori M,Rafikova O,Rafikov R

Thu Feb 06 00:00:00 UTC 2020

Polysaccharides from Armillariella tabescens mycelia ameliorate insulin resistance in type 2 diabetic mice.

Food & function

Yang R,Li Y,Cai J,Ji J,Wang Y,Zhang W,Pan W,Chen Y

Wed Nov 18 00:00:00 UTC 2020

Effect of 1-Deoxynojirimycin Isolated from Mulberry Leaves on Glucose Metabolism and Gut Microbiota in a Streptozotocin-Induced Diabetic Mouse Model.

Journal of natural products

Hu TG,Wen P,Shen WZ,Liu F,Li Q,Li EN,Liao ST,Wu H,Zou YX

Fri Aug 23 00:00:00 UTC 2019

Micro-RNA-27a/b negatively regulates hepatic gluconeogenesis by targeting FOXO1.

American journal of physiology. Endocrinology and metabolism

Wang S,Ai H,Liu L,Zhang X,Gao F,Zheng L,Yi J,Sun L,Yu C,Zhao H,Li Y

Fri Nov 01 00:00:00 UTC 2019

Thyroid stimulating hormone increases hepatic gluconeogenesis via CRTC2.

Molecular and cellular endocrinology

Li Y,Wang L,Zhou L,Song Y,Ma S,Yu C,Zhao J,Xu C,Gao L

Fri May 05 00:00:00 UTC 2017

TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.

Scientific reports

Dong F,Ling Q,Ye D,Zhang Z,Shu J,Chen G,Fei Y,Li C

Mon Apr 25 00:00:00 UTC 2016

TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.

Scientific reports

Dong F,Ling Q,Ye D,Zhang Z,Shu J,Chen G,Fei Y,Li C

Mon Apr 25 00:00:00 UTC 2016

Betaine supplementation in maternal diet modulates the epigenetic regulation of hepatic gluconeogenic genes in neonatal piglets.

PloS one

Cai D,Jia Y,Song H,Sui S,Lu J,Jiang Z,Zhao R

Thu Nov 12 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: HGNC:8725; mtPCK2; PEP carboxykinase; PEPCK-M; phosphoenolpyruvate carboxykinase 2 (mitochondrial); Phosphoenolpyruvate carboxykinase 2, mitochondrial; Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase

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Gene Aliases: 1810010O14Rik; 9130022B02Rik; PCK2; PEPCK; PEPCK-M; PEPCK2

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UniProt ID: (Human) Q16822, (Mouse) Q8BH04

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Entrez Gene ID: (Human) 5106, (Mouse) 74551, (Rat) 361042

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Function(s)

phosphoenolpyruvate carboxykinase activity phosphoenolpyruvate carboxykinase (GTP) activity protein binding GTP binding metal ion binding nucleotide binding lyase activity carboxy-lyase activity purine nucleotide binding

Process(es)

gluconeogenesis pyruvate metabolic process oxaloacetate metabolic process NADH oxidation positive regulation of insulin secretion response to glucocorticoid cellular response to glucose stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PCK2 Polyclonal Antibody

PCK2 Antibody (14892-1-AP) in ICC/IF

Product Details

14892-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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