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Invitrogen
PCK2 Monoclonal Antibody (3F7)
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FIGURE: 1 / 7
PCK2 Antibody (MA5-49278) in ICC/IF
Immunocytochemistry analysis of PCK2 in HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in PCK2 Monoclonal antibody (Product # MA5-49278) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for ... View More
Product Details
MA5-49278
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
3F7
Immunogen
E.coli-derived human PCK2 recombinant protein (Position: M1-M640).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074936
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HEPG2 whole cell, human Hela whole cell, human HEK293 whole cell, human A431 whole cell, monkey COS-7 whole cell, rat kidney tissue, mouse kidney tissue, mouse Neuro-2a whole cell. IHC: human prostate cancer tissue, human breast cancer tissue, human retcal cancer tissue. ICC/IF: HEPG2 cell. Flow: MCF-7 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:8725; mtPCK2; PEP carboxykinase; PEPCK-M; phosphoenolpyruvate carboxykinase 2 (mitochondrial); Phosphoenolpyruvate carboxykinase 2, mitochondrial; Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase
Gene Aliases: 1810010O14Rik; 9130022B02Rik; PCK2; PEPCK; PEPCK-M; PEPCK2
UniProt ID: (Human) Q16822, (Mouse) Q8BH04
Entrez Gene ID: (Human) 5106, (Mouse) 74551, (Rat) 361042
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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