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PCNA Antibody (600-401-968) in WB
Western blot using Rocklands affinity purified anti-PCNA antibody shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts. Approximately 25 µg of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. After blocking, the membrane was incubated with the primary antibody diluted to 1:1000. The membrane was washed and reacted with a 1:10,000 dilution of IRDye® 800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 (611-132-122) (800 nm channel, green). Molecula... View More
Product Details
600-401-968
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Fish,
Human,
Mouse,
Non-human primate,
Rat,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PCNA protein.
Conjugate
Form
Liquid
Concentration
0.97 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Directed against human PCNA protein. A BLAST analysis was used to suggest cross reactivity with PCNA protein from human, monkey, dog, mouse, rat, bovine, chicken, fish and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen
Gene Aliases: ATLD2; BOS_13036; PCNA; Pcna/cyclin; PCNAR
UniProt ID: (Bovine) Q3ZBW4, (Human) P12004, (Chicken) Q9DEA3, (Rat) P04961, (Mouse) P17918
Entrez Gene ID: (Bovine) 515499, (Dog) 477166, (Human) 5111, (Chicken) 373984, (Rat) 25737, (Mouse) 18538
purine-specific mismatch base pair DNA N-glycosylase activity
DNA polymerase processivity factor activity
receptor tyrosine kinase binding
dinucleotide insertion or deletion binding
MutLalpha complex binding
identical protein binding
chromatin binding
damaged DNA binding
protein binding
enzyme binding
estrogen receptor binding
histone acetyltransferase binding
DNA polymerase binding
transcription factor binding
DNA binding
DNA replication
regulation of DNA replication
mismatch repair
translesion synthesis
positive regulation of deoxyribonuclease activity
negative regulation of transcription from RNA polymerase II promoter
leading strand elongation
base-excision repair, gap-filling
heart development
epithelial cell differentiation
response to lipid
response to cadmium ion
cellular response to xenobiotic stimulus
DNA repair
cellular response to DNA damage stimulus
cellular response to UV
positive regulation of DNA repair
positive regulation of DNA replication
regulation of catalytic activity
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
telomere maintenance via recombination
telomere maintenance
DNA strand elongation involved in DNA replication
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
cell proliferation
protein sumoylation
replication fork processing
nucleotide-excision repair, DNA incision
error-prone translesion synthesis
DNA damage response, detection of DNA damage
error-free translesion synthesis
mitotic telomere maintenance via semi-conservative replication
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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