Bethyl Laboratories

PCNA Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

A300-276A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:10,000
-

Immunohistochemistry (IHC)

1:2,000-1:10,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000-1:10,000
-

Flow Cytometry (Flow)

0.03 µg/1x10^6 cells in 150 µL
-

Immunoprecipitation (IP)

2-5 µg/mg lysate
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residues 75 and 125 of human proliferating cell nuclear antigen.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

phosphate/tris citrate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Rat, Chicken and Bovine

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen

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Gene Aliases: ATLD2; PCNA

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UniProt ID: (Human) P12004, (Mouse) P17918

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Entrez Gene ID: (Human) 5111, (Mouse) 18538

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Function(s)
purine-specific mismatch base pair DNA N-glycosylase activity chromatin binding damaged DNA binding protein binding enzyme binding estrogen receptor binding DNA polymerase processivity factor activity receptor tyrosine kinase binding dinucleotide insertion or deletion binding MutLalpha complex binding histone acetyltransferase binding identical protein binding DNA polymerase binding DNA binding transcription factor binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter DNA replication leading strand elongation regulation of DNA replication DNA repair base-excision repair, gap-filling mismatch repair cellular response to DNA damage stimulus translesion synthesis positive regulation of deoxyribonuclease activity response to lipid cellular response to UV positive regulation of DNA repair positive regulation of DNA replication regulation of catalytic activity cellular response to xenobiotic stimulus G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle telomere maintenance via recombination telomere maintenance DNA strand elongation involved in DNA replication transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest heart development cell proliferation protein sumoylation epithelial cell differentiation replication fork processing nucleotide-excision repair, DNA incision error-prone translesion synthesis DNA damage response, detection of DNA damage response to cadmium ion error-free translesion synthesis mitotic telomere maintenance via semi-conservative replication
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