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          • Primary Antibodies ›
          • PCNA Antibodies

          Invitrogen

          PCNA Monoclonal Antibody (PC10)

          Advanced Verification
          67 Published Figures
          123 References
          View all (139) PCNA antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite PCNA Monoclonal Antibody (PC10)

          • Antibody Testing Data (8)
          • Published Figures (67)
          • Advanced Verification (2)
          PCNA Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          PCNA Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 77

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          PCNA Antibody (13-3900) in ICC/IF

          Immunofluorescent analysis of PCNA was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PCNA Mouse Monoclonal Antibody (Product # 13-3900) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at roo... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          PCNA Antibody in Immunocytochemistry (ICC/IF)
          PCNA Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PCNA Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PCNA Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PCNA Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Immunocytochemistry (ICC/IF)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Immunocytochemistry (ICC/IF)
          PCNA Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          PCNA Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          PCNA Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          PCNA Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody
          PCNA Antibody

          Product Details

          13-3900

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-1 µg/mL
          View 26 publications 26 publications

          Immunohistochemistry (IHC)

          -
          View 49 publications 49 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          View 22 publications 22 publications

          Immunocytochemistry (ICC/IF)

          1-2 µg/mL
          View 7 publications 7 publications

          Immunoprecipitation (IP)

          2-5 µg
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 17 publications 17 publications
          Product Specifications

          Species Reactivity

          Human, Insect, Mouse, Non-human primate, Rat, Yeast

          Published species

          Avian, Fruit fly, Guinea pig, Human, Mouse, Pig, Rabbit, Rat, Shark, Xenopus, Yeast, Zebrafish

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          PC10

          Immunogen

          Rat recombinant PCNA polypeptides.

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor Plus 488 
          • Biotin 
          • FITC
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          purified

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533016

          Target Information

          PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Pol30p; Proliferating cell nuclear antigen

          View more View less

          Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR; POL30; YBR0811; YBR088C

          View more View less

          UniProt ID: (Human) P12004, (Baker's yeast) P15873, (Rat) P04961, (Mouse) P17918

          View more View less

          Entrez Gene ID: (Human) 5111, (Baker's yeast) 852385, (Rat) 25737, (Mouse) 18538

          View more View less

          Function(s)
          purine-specific mismatch base pair DNA N-glycosylase activity chromatin binding damaged DNA binding protein binding enzyme binding estrogen receptor binding DNA polymerase processivity factor activity receptor tyrosine kinase binding dinucleotide insertion or deletion binding MutLalpha complex binding histone acetyltransferase binding identical protein binding DNA polymerase binding DNA binding transcription factor binding
          Process(es)
          meiotic mismatch repair DNA replication leading strand elongation lagging strand elongation regulation of DNA replication DNA repair nucleotide-excision repair mismatch repair postreplication repair chromatin silencing at telomere cellular response to DNA damage stimulus mitotic sister chromatid cohesion chromatin silencing at silent mating-type cassette negative regulation of transcription from RNA polymerase II promoter base-excision repair, gap-filling heart development translesion synthesis epithelial cell differentiation positive regulation of deoxyribonuclease activity response to lipid response to cadmium ion cellular response to xenobiotic stimulus cellular response to UV positive regulation of DNA repair positive regulation of DNA replication regulation of catalytic activity G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle telomere maintenance via recombination telomere maintenance DNA strand elongation involved in DNA replication transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell proliferation protein sumoylation replication fork processing nucleotide-excision repair, DNA incision error-prone translesion synthesis DNA damage response, detection of DNA damage error-free translesion synthesis mitotic telomere maintenance via semi-conservative replication
          It has to be done as per old AB suggested Products section.
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