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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PCNA Antibodies

          Invitrogen

          PCNA Monoclonal Antibody (PC10), Biotin

          Advanced Verification
          1 Published Figure
          137 References
          View all (139) PCNA antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite PCNA Monoclonal Antibody (PC10), Biotin

          • Antibody Testing Data (7)
          • Published Figures (1)
          • Advanced Verification (1)
          PCNA Antibody in Western Blot (WB)
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          PCNA Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          PCNA Antibody (MA5-11355) in WB

          Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), Jurkat (Lane 3), HEK-293 (Lane 4), NIH/3T3 (Lane 5), MDA-MB-231 (Lane 6), A549 (Lane 7), SH-SY5Y (Lane 8) and Raji (Lane 9). The blot was probed with anti-PCNA Monoclonal Antibody (Product # MA5-11355, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 34 kDa band corresponding to PCNA was ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Immunocytochemistry (ICC/IF)
          PCNA Antibody in Immunocytochemistry (ICC/IF)
          PCNA Antibody in Flow Cytometry (Flow)
          PCNA Antibody in Immunoprecipitation (IP)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody

          Product Details

          MA5-11355

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500
          View 29 publications 29 publications

          Immunohistochemistry (IHC)

          -
          View 96 publications 96 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200-1:400
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:20-1:50
          View 8 publications 8 publications

          Flow Cytometry (Flow)

          1:200-1:1,000
          -

          Immunoprecipitation (IP)

          2 µg/mL
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Rat

          Published species

          Amphibian, Cat, Dog, Guinea pig, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Rhesus monkey, Xenopus, Yeast, Zebrafish

          Host/Isotype

          Mouse / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          PC10

          Immunogen

          Recombinant rat PCNA protein

          Conjugate

          Biotin Biotin Biotin
          • Unconjugated
          • Request custom conjugation

          Form

          Liquid

          Concentration

          200 µg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4, with 0.2% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10984046

          Product Specific Information

          Methanol fixation is required for immunofluorescence and flow cytometry staining. To decrease nonspecific staining for both applications, using a Biotin/Streptavidin blocker is recommended.

          By WB, MA5-11355 detects a predominant band between 30-35kD, and fainter nonspecific bands at ~72kD and 130kD.

          Target Information

          PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen

          View more View less

          Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR

          View more View less

          UniProt ID: (Human) P12004, (Rat) P04961

          View more View less

          Entrez Gene ID: (Human) 5111, (Rat) 25737

          View more View less

          Function(s)
          purine-specific mismatch base pair DNA N-glycosylase activity chromatin binding damaged DNA binding protein binding enzyme binding estrogen receptor binding DNA polymerase processivity factor activity receptor tyrosine kinase binding dinucleotide insertion or deletion binding MutLalpha complex binding histone acetyltransferase binding identical protein binding DNA polymerase binding transcription factor binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter leading strand elongation regulation of DNA replication base-excision repair, gap-filling mismatch repair heart development translesion synthesis epithelial cell differentiation positive regulation of deoxyribonuclease activity response to lipid response to cadmium ion cellular response to xenobiotic stimulus G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle telomere maintenance via recombination telomere maintenance DNA strand elongation involved in DNA replication transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell proliferation protein sumoylation replication fork processing nucleotide-excision repair, DNA incision cellular response to UV error-prone translesion synthesis DNA damage response, detection of DNA damage positive regulation of DNA repair positive regulation of DNA replication error-free translesion synthesis mitotic telomere maintenance via semi-conservative replication
          It has to be done as per old AB suggested Products section.
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