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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PCNA Antibodies

          Invitrogen

          PCNA Monoclonal Antibody (PC10)

          Advanced Verification
          17 Published Figures
          204 References
          View all (139) PCNA antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite PCNA Monoclonal Antibody (PC10)

          • Antibody Testing Data (13)
          • Published Figures (17)
          • Advanced Verification (1)
          PCNA Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          PCNA Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 31

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          PCNA Antibody (MA5-11358) in ICC/IF

          Immunofluorescent analysis of PCNA (purple) in HeLa cells. The cells were fixed and permeabilized with ice-cold methanol for 15 minutes, and blocked with 1% Blocker BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a PCNA monoclonal antibody (Product # MA5-11358) at a dilution of 1:50 in 1% Blocker BSA in PBS (right panel) or incubated in blocking buffer alone as a negative control (left panel) for 1 hour at room temperature, and then incubated with a DyLight 650-conjugated goat anti-mouse IgG seconda... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          PCNA Antibody in Immunocytochemistry (ICC/IF)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PCNA Antibody in Flow Cytometry (Flow)
          PCNA Antibody in Immunoprecipitation (IP)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Flow Cytometry (Flow)
          PCNA Antibody in Flow Cytometry (Flow)
          PCNA Antibody in Immunoprecipitation (IP)
          PCNA Antibody in Immunocytochemistry (ICC/IF)
          PCNA Antibody in Immunocytochemistry (ICC/IF)
          PCNA Antibody in Immunocytochemistry (ICC/IF)
          PCNA Antibody in Immunocytochemistry (ICC/IF)
          PCNA Antibody in Immunocytochemistry (ICC/IF)
          PCNA Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PCNA Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PCNA Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Immunohistochemistry (IHC)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody in Western Blot (WB)
          PCNA Antibody

          Product Details

          MA5-11358

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:50
          View 44 publications 44 publications

          Immunohistochemistry (IHC)

          -
          View 107 publications 107 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200-1:400
          View 19 publications 19 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:50-1:100
          View 13 publications 13 publications

          Flow Cytometry (Flow)

          1:2-1:4
          -

          Immunoprecipitation (IP)

          1:25
          View 3 publications 3 publications

          in situ PLA (PLA)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 16 publications 16 publications
          Product Specifications

          Species Reactivity

          Dog, Human, Mouse, Non-human primate, Rat

          Published species

          Amphibian, Avian, Cat, Dog, Fish, Guinea pig, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Rhesus monkey, Xenopus, Yeast, Zebrafish

          Host/Isotype

          Mouse / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          PC10

          Immunogen

          Recombinant rat PCNA protein

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Biotin 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          20 µg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4, with 0.2% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10982348

          Product Specific Information

          Methanol fixation is required for IF staining.

          Target Information

          PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen

          View more View less

          Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR

          View more View less

          UniProt ID: (Human) P12004, (Rat) P04961, (Mouse) P17918

          View more View less

          Entrez Gene ID: (Dog) 477166, (Human) 5111, (Rat) 25737, (Mouse) 18538

          View more View less

          Function(s)
          purine-specific mismatch base pair DNA N-glycosylase activity chromatin binding damaged DNA binding protein binding enzyme binding estrogen receptor binding DNA polymerase processivity factor activity receptor tyrosine kinase binding dinucleotide insertion or deletion binding MutLalpha complex binding histone acetyltransferase binding identical protein binding DNA polymerase binding transcription factor binding DNA binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter leading strand elongation regulation of DNA replication base-excision repair, gap-filling mismatch repair heart development translesion synthesis epithelial cell differentiation positive regulation of deoxyribonuclease activity response to lipid response to cadmium ion cellular response to xenobiotic stimulus DNA replication DNA repair cellular response to DNA damage stimulus cellular response to UV positive regulation of DNA repair positive regulation of DNA replication regulation of catalytic activity G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle telomere maintenance via recombination telomere maintenance DNA strand elongation involved in DNA replication transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell proliferation protein sumoylation replication fork processing nucleotide-excision repair, DNA incision error-prone translesion synthesis DNA damage response, detection of DNA damage error-free translesion synthesis mitotic telomere maintenance via semi-conservative replication
          It has to be done as per old AB suggested Products section.
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