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Invitrogen
PCQAP Polyclonal Antibody
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PCQAP Antibody (PA5-79656) in ICC/IF
Immunocytochemistry analysis of MED15 using anti-MED15 antibody (Product # PA5-79656). MED15 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-MED15 antibody (Product # PA5-79656) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Vi... View More
Product Details
PA5-79656
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human MED15 recombinant protein (Position: M1-A285).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746771
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, huamn K562 whole cell, human Hela whole cell. IHC: human colon cancer tissue, human lung cancer tissue, human placenta tissue, mouse brain tissue, rat brain tissue. ICC/IF: U20S cell. Flow: U20S cell.
Target Information
This gene encodes a transcription factor that represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcriptional activity. Defects in this gene are a cause of tricho-rhino-phalangeal syndrome (TRPS) types I-III.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activator-recruited cofactor 105 kDa component; activator-recruited cofactor, 105-kD; ARC105; CTG repeat protein 7a; DKFZp686A2214; DKFZp762B1216; FLJ42282; FLJ42935; Mediator complex subunit 15; Mediator of RNA polymerase II transcription subunit 15; PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; PC2-glutamine-rich-associated protein; Positive cofactor 2 glutamine/Q-rich-associated protein; positive cofactor 2, glutamine/Q-rich-associated protein; positive cofactor 2, multiprotein complex, glutamine/Q-rich-associated protein; TIG-1; TPA inducible gene-1; TPA inducible protein; TPA-inducible gene 1 protein; trinucleotide repeat containing 7; Trinucleotide repeat-containing gene 7 protein
Gene Aliases: A230074L19Rik; ARC105; AW536074; CAG7A; CTG7A; MED15; mPcqap; PCQAP; TIG-1; TIG1; TNRC7
UniProt ID: (Human) Q96RN5, (Mouse) Q924H2
Entrez Gene ID: (Human) 51586, (Rat) 360743, (Mouse) 94112
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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