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PCSK4 Antibody (PA5-79788) in WB
Western blot analysis of PCSK4 in, Lane 1: rat brain lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with PCSK4 Polyclonal Antibody (Product # PA5-79788) at 0.5 μg/mL overnight a... View More
Product Details
PA5-79788
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Proprotein convertase PC4 (117-133aa DPWFSKQWYMNKEIQQD).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746903
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain.
Target Information
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Plays a role in transcriptional coactivation. May be involved in stabilizing the multiprotein transcription complex.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: KEX2-like endoprotease 3; NEC 3; Neuroendocrine convertase 3; PC4; Prohormone convertase 3; Proprotein convertase subtilisin/kexin type 4
Gene Aliases: AI647044; L21221; NEC 3; Nec-3; NEC3; PC4; Pcsk4; SPC5
UniProt ID: (Rat) Q78EH2, (Mouse) P29121
Entrez Gene ID: (Rat) 171085, (Mouse) 18551
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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